Quality Control Molecular Biology

Veera Manukonda

Cary, NC

Email: *************@*****.***

Mob: +1-319-***-****

Medical Laboratory Specialist

MB(ASCP)-certified biotechnologist with a Master’s degree and expertise in molecular diagnostics, including qPCR, RT-PCR, and ELISA, provides precise and reliable test results. At Oklahoma State University, I maintained and calibrated laboratory equipment, which improved workflow efficiency and ensured compliance with quality standards. I am skilled in basic microbiological techniques, such as Gram staining, and I can apply molecular skills to microbiology through pathogen detection assays. I have experience in quality control, developing SOPs, and training laboratory staff, which supports Duke Health’s focus on education and innovation. I am ready to help with accurate diagnostics and work well in a CAP-accredited laboratory. SKILLS

Molecular Biology:

• Recombinant cloning (PCR, qPCR, RT-PCR etc.)

• Generation of mutant’s (Including SDM strategies)

• Viral Vector Design: Engineering vectors for gene therapy (e.g., Adenovirus, lentivirus, Retro virus)

• DNA/RNA Extraction and Purification

• Southern and Northern blotting

• Basic knowledge of nucleic acid sequencing

Biochemistry:

• Protein extraction and purification

• SDS-PAGE and Western blotting

• Enzyme assays and kinetics

• Spectrophotometry (UV/Vis) and fluorometry

• Basic chromatography techniques (Affinity, Ion-Exchange and Gel Filtration chromatography, HPLC, FPLC)

• Protein engineering and mutagenesis

• Post-translational modifications analysis (Labeling biomolecules, protein-protein interactions: BioID and TurboID etc )

• Biophysical techniques (ITC, SPR)

Cell Biology:

• Cell culture techniques (maintenance, passaging)

• Microscopy (light, fluorescence)

• Cell viability and proliferation assays

• Staining techniques (DAPI, immunocytochemistry)

• Basic flow cytometry

• Understanding of cell signaling pathways

• Functional assays for receptor activity

• Generation of transient or stable cell lines via transfection or viral transduction.

Clinical and Diagnostic skills:

• Immunoassay techniques (ELISA, rapid tests)

• Basic microbiological techniques (culture methods, Gram staining)

• Basic molecular diagnostic techniques (PCR setup, gel electrophoresis for diagnostics)

• CLIA/CAP Compliance

• Quality Control (QC) and Quality Assurance (QA)

• Molecular Diagnostics: Designing/validating qRT-PCR assays, multiplex PCR, and digital droplet PCR for pathogen detection or genetic mutations. Certificates:

• MB(ASCP) certification (Valid through 02/2028)

• Python for Genomic Data Science (Johns Hopkins University)

• Genomic Technologies (Johns Hopkins University)

• SQL for Data Science (University of California, Davis) PROFESSIONAL SUMMARY

Syngenta Crop Lab. RTP, NC • 11/2023 - 06/2024 Research Assistant-3(Contract)

• Used molecular biology methods like PCR, qPCR, and gel electrophoresis to validate the project’s research objectives.

• Implemented laboratory techniques to analyze data independently, which improved workflow efficiency and reporting accuracy for research publications.

• Improved laboratory compliance by setting up a strict review process for test results, ensuring accuracy and reliability.

• Maintained an organized and safe laboratory environment, following safety protocols and boosting operational efficiency. University of North Carolina • Chapel Hill, NC • 09/2022 - 04/2023 Research Associate

• Led training sessions for new hires, increasing team productivity by 20% through better processes and skill development.

• Carried out precise instrumentation calibration procedures, leading to a 25% improvement in test reliability while meeting project deadlines.

• Spearheaded a compliance training initiative that raised awareness of regulatory requirements, promoting a culture of safety and responsibility within the laboratory team.

• Created and expressed recombinant proteins in bacterial and mammalian cell lines, improving experimental outcomes and efficiency. Oklahoma State University • Tulsa, OK • 11/2021 - 08/2022 Research Associate

• Accelerated project delivery by using technical skills and critical thinking in team efforts.

• Developed and applied troubleshooting strategies for laboratory incidents, improving compliance and accuracy through systematic root cause analysis.

• Purified proteins using affinity and ion exchange chromatography, increasing yield through improved protocols.

• Boosted analytical test reporting accuracy by implementing automated equipment and strict quality control measures.

• Studied ADAMTS metalloproteases' roles in cardiovascular diseases, improving understanding of hemostasis and matrix remodeling.

• Resolved equipment issues, significantly enhancing workflow efficiency and ensuring compliance with quality standards throughout the laboratory. EDUCATION

MSc in Biotechnology

Acharya Nagarjuna University • Guntur, Andhra Pradesh, India CERTIFICATIONS

MB (ASCP)CM • 02/2025 - 02/2028

BoC

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