Scientist

MOHAMMAD SHAHNAIJ

***** ** **** **, ***** Gardens, FL-33055

Email: ************@*****.***, Phone:507-***-**** Have 10+ years of experience in bacteriology, virology, immunology and cancer laboratories of world-renowned institutions. Precise and consistent in clinical and routine sample analysis following standard operating procedures, using sophisticated analytical instrumentation such as ELISA, HPLC, FACS, qPCR, WB, IHC and so on. Skilled in maintaining clear and concise laboratory records. Familiar with cGMP, GLP, OSHA, and regulatory agencies. Experienced in the investigation of out-of-specification results. Capable to communicate results, problems, or issues verbally as well as in writing both within and between departments. Thoroughly experienced with ImageStudio, Zeiss, FlowJo, MS office applications, SAP software. Having excellent leadership ability and flexibility to easily adapt in a teamwork role. EDUCATION

Ph.D. in Applied immunology in Pharmaceutical Science Sep 2018 - Sep 2021 Kanazawa University, Japan

B.Sc. and M.S. in Biochemistry and Molecular Biology Jun 2005 - Dec 2012 University of Dhaka, Bangladesh

EXPERIENCE

SKILLS

1.Bacterial culture, Antimicrobial susceptibility testing, Next generation sequence data analysis 2.Mammalian cell culture, 3D Organoid/Spheroid culture, CRISPR-Cas9 editing, Western blot, Immunostaining, Confocal Microscopy, Cytokine bioassay 3.Handling human and mouse samples, cancer implant, in vivo imaging study 4.Liver perfusion, ELISA, radiation, immunotherapy, Flowcytometry, FACS analysis 5.Cloning, transfection, transduction, protein purification, HPAEC/anion exchanger 6.Computational skills- FlowJo, imageJ, BASH, SPSS, Graphpad prism, Rstudio etc. Department of Radiation Oncology, University of Miami 2024-25 Postdoctoral Associate, Sylvester Comprehensive Cancer Center

● Developed liver and prostate cancer model in mice.

● Analyzed immune responses after radiotherapy & immunotherapy

● Trained technicians to perform FACS, immunostaining & qPCR Enteric Neuroscience Program lab, Mayo clinic 2021-23 Postdoctoral Research Fellow, Physiology and Biomedical Engineering

● CRISPR-Cas9 editing of epigenomics marks

● Detected the regulatory pathways of ion-transporter protein

● Lentivirus production to transduce Cas9, R-GECO, GCaMP etc Immunology lab, Kanazawa University 2018-21

Graduate fellow

● Clone antigen expressing plasmid and viral vectors

● Produce and purify Adenovirus, AAV, Baculo and Vaccinia viruses

● Produced antibodies, protein and antigens in bacteria

● Developed vaccine and treatment strategies providing 100% protection.

● T cell mediated immune response to infectious disease International Centre for Diarrhoeal Disease Research, Bangladesh (icddrb) 2012-18 Research officer

● Characterized two novel serotypes of gut pathogens

● Reported antimicrobial resistance in gut bacteria

● Genomic/NGS analysis of thousands of enteric pathogens

● Genetic polymorphism and immune response of Guillain Barre syndrome

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