Research Assistant Biomedical Engineering

Ming Guan (Taiwan)

**** ******** **** *** ***, Durham, NC, 27705

************@*****.*** ~ Cell: (949) 572 – 0920

EDUCATION

Duke University, Durham, NC Graduated May 2025

Master’s Degree, major in Biomedical Engineering concentrating in Tissue Engineering and Regenerative Medicine GPA: 3.65

Courses: Cardiovascular System Engineering, Environmental Molecular Biotechnology (with lab), Principles in Immunology, Comprehensive Immunology, Applied Partial Differential Equation & Complex Variables, Quantitative Cell & Tissue Engineering

Northeastern University, Boston, MA Graduated May 2023 Bachelor of Science Degree in Bioengineering concentrating in Cellular and Tissue Engineering Minor in Mathematics

GPA: 3.70

Activities: NU In exchange program to London (2018 Fall) Honors: Dean’s List (Spring/Fall 2019, Spring/Fall2020, Fall 2021) COMPUTER & LANGUAGE SKILLS

Applications: AutoCAD, MATLAB, Microsoft Word, Microsoft PowerPoint, Microsoft Excel Languages: English, Mandarin, Japanese

Experience with Matlab:

o Specialized in analyzing data, generating figures, and modeling o Recent application: Implementing Clancy Markov Model to simulate ECG graph for patients with LQT3 and how different drugs can impact patients’ recoveries. LABORATORY EXPERIENCE

Professor Jiahe Li’s lab (molecular engineering), Boston, MA 2021 April to 2023 April o Research Assistant in Professor Li’s lab for 2 years o Responsible for daily laboratory management: ordering/tracking supplies, autoclave, preparing media/agarose, SDS & Urea page, and biohazard disposal.

o Specialized in cloning techniques including plasmid miniprep, Genomic DNA extraction, bacterial transformation, Gel electrophoresis, TCA precipitation, Western blot, RNA extraction, hifi assembly, basic and colony PCRs.

o Specialized in cell culturing, cell passage and co-culturing with bacterial cells. Able to operate anaerobic chambers.

o Additional supervised animal procedures including euthanasia, mouse husbandry, breeding, and inspections. PUBLICATIONS

o Contributing author of “Nanobody-Functionalized Cellulose for Capturing SAR-CoV-2” (DOI: https://doi.org/10.1128/aem.02303-21, Applied and Environmental Microbiology) o Contributing author of “Engineered Bacillus subtilis as Oral Probiotics To Enhance Clearance of Blood Lactate” (DOI: https://doi.org/10.1021/acssynbio.4c00399, ACS Synthetic Biology) o Contributing author of “Non-pathogenic E.coli displaying decoy-resistance IL18 mutein boosts anti-tumor and CAR NK cell responses” (DOI: https://doi.org/10.1038/s41587-024-02418-6, Nature Biotechnology) VOLUNTEER EXPERIENCE:

o Lenox Camden Apartment Afterschool Program (K-12 Education), Boston, USA 2021 Jan to 2021 April o YMCA Charity Shop in London, London, England 2018 Aug to 2018 Dec