Microbiology Lab Cell Biology

Allen Badolian Email: *************@*****.*** Tel: 714-***-****

California State University, Fullerton Fall 2021-current

• Evolutionary Biochemistry of the Cellular Stress Response Laboratory

• Principal Investigator: Dr. Nikolas Nikolaidis

Allen Badolian

2295 N. Tustin St. Unit 72, Orange, CA, 92865

Email/Personal: *************@*****.*** Email/Academic: **.**@***.*********.*** Linkedln: https://www.linkedin.com/in/allen-badolian-b14a58245/ Personal Statement

Active researcher in Hsp70 translocation to the plasma membrane and extracellular milieu during cellular stress at California State University, Fullerton. Former Teaching Associate in California State University, Fullerton College of Natural Science and Mathematics, in Biological Science). Graduate of California State University, Bachelor of Science in Cell and Developmental Biology, Master of Science in Biology. Passionate about healthcare and the pre-clinical pipeline. My passion is to inspire others, which drives, modus operandi. Experienced in the hospital and the laboratory. Education and Select Highlights

California State University, Fullerton 2018-2021

Bachelor of Science Degree, Cell and Developmental Dean’s List @ 3.62 GPA 2018-2021

California State University, Fullerton 2021-2025

Master of Science Degree, Biology

President of Biology Graduate Student’s Club

Dean’s List @ 3.85 GPA 2021-2025

Selected Coursework

Mammalian Physiology Biological Chemistry Cell Biology Stem Cell Biology

Teaching

Microbiology

Organic Chemistry

Genetics

Cancer Biology

Selected Research

Selected Academic Publications

Badolian, Allen. (2025). Mechanisms of HSPA1A Export to the Extracellular Medium in Heat-Shocked Cells. ScholarWorks (The California State University). https://doi.org/10.5281/zenodo.15460255

Allen Badolian Email: *************@*****.*** Tel: 714-***-**** Selected Academic Publications Continued

Arce, Alberto, Altman, Rachel, Badolian, Allen, Low, Jensen, Cuaresma, Azalea Blythe, Keshet, Uri, Stahelin, Robert, Nikolaidis, Nikolas. (2025). Heat Shock-Induced PI(4)P Increase Drives HSPA1A Translocation to the Plasma Membrane in Cancer and Stressed Cells through PI4KIII Alpha Activation. bioRxiv : the preprint server for biology. https://doi.org/10.1101/2025.02.16.638537

(Primary Author/Equal Contributor)

Low, Jensen, Altman, Rachel, Badolian, Allen, Cuaresma, Azalea Blythe, Briseno, Carolina, Keshet, Uri, Fiehn, Oliver, Stahelin, Robert V., Nikolaidis, Nikolas. (2024). Heat-Induced Phosphatidylserine Changes Drive HSPA1A's Plasma Membrane Localization. BioRxiv: the preprint server for biology. https://doi.org/10.1101/2024.12.02.626454

(Primary Author/Equal Contributor)

Badolian, Allen. (2024). Deciphering the Role of Lipids in Regulating Plasma Membrane and Extracellular HSPA1A. Journal of Biological Chemistry, 10.1016/j.jbc.2024.106332

(Primary Author/Equal Contributor)

Selected and Relevant Conference Presentations

2025 American Society for Biochemistry and Molecular Biology (ASBMB) Badolian, A, Arce, A., Briseno, C., Nikolaidis, N. (2025, Apr 13). Investgating the Lipid Dependent Regulation and Therapeutic Targeting of Plasma Membrane and Extracellular HSPA1A.

Poster presented at both Poster Session and Spotlight Presentation/Panel on New Foundational Discoveries in Phospholipids at the ASBMB 2025, Chicago, Il. 2025 California State University Biotechnology Symposium (CSU Biotech) Badolian, Allen, Arce, Alberto, Briseno, Carolina, Nikolaidis, Nikolas. (2025, Jan 7). A Semi-High-Throughput Investigation of the Lipid-Dependency and Therapeutic Potential of Plasma Membrane and Extracellular HSPA1A. Poster presented at CSU Biotech 2025 Annual Symposium in Garden Grove, California. 2024 American Society for Biochemistry and Molecular Biology (ASBMB) Badolian, Allen, Briseno, Carolina, Nikolaidis, Nikolas. (2024, Apr 12). Deciphering the Role of Lipids in Regulating Plasma Membrane and Extracellular HSPA1A. Poster presented at ASBMB Annual Symposium in San Antonio, Texas. 2024 California State University Biotechnology Symposium (CSU Biotech) Badolian, Allen, Nikolaidis, Nikolas. (2024, January 12). Characterizing the lipid-dependency of plasma membrane and extracellular HSPA1A Poster presented at CSU Biotech 2024 Annual Symposium in San Jose, California April 2025,

Chicago, IL

Jan. 2025,

Garden Grove, CA

April 2024,

San Antonio, TX

Jan. 2024,

San Jose, CA

Allen Badolian Email: *************@*****.*** Tel: 714-***-**** Selected and Relevant Roles

Teaching Assistant at California State University Fullerton Fullerton, CA

Biology 191b Laboratory Instructor

• Provided in person laboratory instruction to courses with 25-30 students.

• Assisted in creating learning modules, built presentations, created assignments/exams.

• Utilized organization and time management inside and outside of courses, such as responding to emails, time management, and coordinating students with disabilities.

• Utilized microbiology and physiological techniques i.e., vol. pipetting, Kohler microscopy, histology, hematocrit, blood typing, dilutions, centrifugation, and chemical analysis. Laboratory Technician at California State University Fullerton Fullerton, CA

Full Time Researcher and Technician

• Taught new and existing laboratory researchers in the Nikolaidis laboratory techniques such as confocal microscopy, cell culture, and western blotting.

• Troubleshooted laboratory materials and equipment such as the Promega Imagen ™, Luminometers, Cellometers, Plate Readers, and Mass Spectrometers.

• Led teams of both graduate and undergraduate students through the scientific method, from sample collection, cell lysis, and quantification techniques and tools such as SDS PAGE, confocal microscopy, western blotting, and ELISA.

• Learned and taught both graduate and undergraduate students how to create graphs and analyze their data using Microsoft Tools (Illustrator, Word, Photoshop, PowerPoint), ImageStudio, RStudio, ImageJ, BoxPlotR, and ANOVA. Selected Experiences

Saint Joseph of Orange (Providence Network), Orange, CA ER Main Volunteer

• Direct and assist all patients entering the emergency care center (ECC)

(200-250 patients daily) with registration (via the integrated campus system) and transcribe care instructions.

• Assist nurses and physicians with movement of samples and medical equipment across triage, EKG rooms, fast track rooms, and general patient rooms to designated regions.

• Handle proper equipment, including but not limited to moving beds, making beds, oxygen supplies, basic medical equipment, and securing IVs. Saint Joseph of Orange (Providence Network), Orange, CA OB Volunteer

• Transport patients to and from various sections throughout the hospital

• Provide support to patients by bringing care and comfort items while utilizing patient conduct.

• Assist nurses with IDing and badge creation for patients and visitors. UNICEF at California State University, Fullerton, Fullerton, CA Board Member

• Coordinate with others in leadership positions and utilize communication skills to run meetings and collect bulk data via modern computer platforms. August 2021- Present

August 2021- Present

March 2019-April 2021

January 2018-March 2019

October 2017- May 2019

Allen Badolian Email: *************@*****.*** Tel: 714-***-**** Selected Interpersonal Skills

• Organized person in and outside of the lab. An ability to handle multiple fast paced projects at once.

given different coursework with laboratory components as well as lecture components.

• Bilingual (English, Armenian) and cooperative in team environments as well as individually in laboratory settings within both chemical and cellular laboratory environments.

• Hundreds of hours of laboratory training, wet lab, and computational data analysis Selected Laboratory/Research Conduct Skills

• Sterilize and clean glassware, including disks, tubes, beakers, etc. in a biosafety level 2 laboratory.

Select Specialty Laboratory Skills:

I. Cellular/Physiological

• Prepare samples (proficient in dye-based staining) and operate electron and std microscopes.

• Draw blood samples from human participants and analyze components. II. Microbiological

• Competent in fluorescent tagging in a basic yeast or bacterial sample.

• Proficient in plaque assaying techniques and counting. III. Genetics

• Prepare and purify DNA and preform mutagenesis analysis.

• Set up samples, label, and place in incubation, as well as centrifugation.

• Perform Transfections, cell culture, and cell death analysis.

• Perform SDS PAGE and Western Blotting.

• Perform cell lysis & cell counts, confocal microscopy, sub-cellular microscopy, data analysis, ImageJ analysis, R Studio analysis, ELISA, RNA interference, Western blotting, Gel electrophoresis, SDS page, molecular cloning & primer design, spectrophotometry. IV. Chemical

• Characterize samples via gas and liquid chromatography, as well as melting point analysis. Additional Awards and Achievements

• Both an International Baccalaureate and a high school diploma.

• Broadcom MASTERS nominee; top 10% of applicants. (May 2017)

• Google “Master of Coding” (August 2019)

• American Legion Distinguished Achievement Award; all-around individual

• Badolian, Allen (May 2016). Efficacy of antibiotics chloramphenicol, tetracycline, and penicillin in combination and individually of everyday ingredients. OC Community Technology Expo. Orange, CA.

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