Scientist I

University of Massachusetts, Lowell

Master of science Biomedical Engineering and Biotechnology (GPA: 3.845) May 2024 Sardar Patel University - India

Master of science Biotechnology (GPA: 8.10) May 2019 Sardar Patel University – India

Bachelor of science Biotechnology (GPA: 8.54) May 2017 Core Competencies

Laboratory techniques: DNA/RNA purification, PCR, molecular cloning, transformation, SDS-PAGE, Western Blot, ELISA (indirect, competitive, whole-cell, serum antibody titer analysis), protein expression and purification, cell lysis (detergent, sonication, homogenization), UV-Vis spectroscopy, fluorescence spectroscopy, protein quantification

(BCA, Bradford), transmission electron microscopy (TEM), immunofluorescence, cytotoxicity assays (MTT, Alamar blue), endotoxin analysis (HEK-Blue TLR4, LAL)

Cell culture: Bacterial culture including full upstream process (USP) for outer membrane vesicle (OMV) production, baculovirus-based protein production with insect cells, mammalian cell culture including CHO, HEK 293 and human cancer cell lines, Cell banking, aseptic lab techniques (BSL1, BSL2) Downstream processes (DSP): Chromatography (affinity, IEX, SEC), AKTA FPLC, HPLC, tangential flow filtration

(TFF), dialysis, ultracentrifugation

Computational Skills: Microsoft office (Excel, Word, PowerPoint), GraphPad Prism, Image J, gene design Soft Skills: Mentorship and training (interns, staff), attention to detail, time management, experimental design Experience

Versatope Therapeutics - Lowell

Scientist I- Vaccine and drug discovery May 2023-Present

• Designed 25+ experiments to optimize OMV conjugation to malarial, group A Strep, and influenza A antigens

• As the lead researcher, designed 25+ experiments, delivered presentations, and reported findings to internal teams

• Employed multistep column chromatography design (affinity, IEX and SEC) achieved >95% purity of protein

• Independently handled the entire USP and DSP for >25 OMV production batches in shake flasks

• Characterized OMV toxicity from endotoxin by the HEK-Blue TLR4 assay (Invivogen)

• Optimized critical process parameters in the baculovirus protein production system to improve quality and yield

• Prepared and delivered journal club presentations on recent developments in vaccine technology

• Efficiently managing multiple projects and tasks with unwavering focus and in fast paced environment Summer Intern-Drug discovery May 2023- Aug 2023

Project: Optimizing drug loading into OMVs for cancer therapy

• Enhanced DNA encapsulation efficiency from 1% to 10% through strategic modifications

• Assisted in development of methods for loading small-molecule drugs into OMVs

• Collaborated with supervisor on all experimental designs and developed capacity for independent decision making LabCorp LLC. - Bedford

Research Associate I - Microbiology Aug 2021- Oct 2022

• Achieved timely project completion in pre-clinical GLP/non-GLP microbiological testing of medical devices

• Performed quality check of medical device by Bioburden, Bio validation, Gram staining, BBL crystal ID etc.

• Reviewed 10+ laboratory protocols and SOPs for USP (US Pharmacopeia) and AAMI regulatory compliance

• Collaborated with cross functional teams to perform cytotoxicity assay and endotoxin assays

• Performed final sterility check of medical devices by efficient aseptic technique in cleanroom

• Independently managed laboratory projects through LIMS, ensuring proper documentation and batch record Education

CHARMI PATEL

Scientist I/ Scientific Researcher/ Vaccine Discovery Chelmsford, MA Charmi Patel LinkedIn **********@*****.*** Bills Biotech - India

Quality Control Intern July 2019 – Oct 2019

• Performed quality analysis of the final product, evaluating texture, pH, moisture content, shelf life and purity by HPLC to ensure product consistency and quality standards

• Ensured 100% regulatory compliance by following GMP and GLP in testing processes

• Conducted product testing on over 8+ batches of Tacrolimus and Mupirocin to ensure their purity and quality, adhering to industry standards

• Maintained organized records of test results and batch data to ensure compliance and traceability Research Projects

Chemistry Department - UMass Lowell

Graduate Student Researcher-Independent study Sep 2023 – Dec 2023

• Project: Optimization of N2A protein extraction from E. coli strain using autoinduction method

• Developed a reproducible protein extraction protocol using a high-pressure homogenizer, IMAC column and HPLC

• Implemented 8 expression modifications from 15 batches to achieve >60% protein purity CSIR-CSMCRI - India

Graduate Student Researcher- Thesis Nov 2018 – Feb 2019

• Performed biochemical assays and mass profiling of the traditional plant Andrographis paniculata Nees

• Analyzed and compared data from stem, leaves, and root samples using statistical and graphical methods

• Conducted 20+ experiments to gather high-quality data, ensuring accuracy and consistency throughout the process

• Successfully identified 32% compounds with medicinal significance in leaves

• Conducted a comprehensive literature review of over 30 studies on the medicinal properties of the identified compound

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