Supply Chain Research Associate

Steven Janasik

San Antonio, TX *****

404-***-**** (c)

**************@*****.***

Education:

Bachelor of Science, Biology in progress

Bachelor of Arts, Biopsychology with University Honors, Northern Illinois University

Associate of General Studies, Joliet Junior College

Associate of Arts, Joliet Junior College

Associate of Science, Joliet Junior College

Skills:

Competent performing in vivo rodent procedures including seventeen behavioral tests, ovarectomies, and cranial cannulations, and post-mortem procedures from euthanasia up to tissue slicing and culture

Experienced in in vitro assays including polymerase chain reaction, restriction fragment length polymorphism, molecular microbial testing, nuclear staining flow cytometry, thrombin generation assays, enzyme linked immunosorbent assays, light transmission aggregometry, DNA isolation and purification, and whole blood hemostasis (ROTEM and TEG)

Extensive experience in blood banking including phlebotomy, sample management, component production, specialized production, lot release, moderate complexity testing, and Supply Chain

Technical Experience:

Instruments-Verify Now platelet reactivity instrumentation, TEG 5000, CAT Thrombinoscope, Mupid-EXu electrophoresis system, Biorad power pac 300 electrophoresis system, UVP Ultraviolet Transluminator, ChemiDoc gel imager, Biotek Synergy 2, Cytation 5 plate reader, Quant Studio 3, Horiba cell counter, PAP-8E Light Transmission Aggregometer, Sysmex CA-600 series, ACL-elite, Quant Studio 12, King Fischer Magmax, SimpliAmp Thermocycler, Accufil, Sysmex XE2300, BD flow cytometer, ThermoScientific UltiMate 3000, Labconco CentriVap Concentrator, centrifuges, expressor, sterile connection devices, Welch Allyn Connex Pro 3400, pH meters, Sarstedt decapper, BacT Alert 3D, microtome, cryostat, Hamilton Kinder, Gustometer, Davis Rig, Smith Hotel, KOPF stereotaxic apparatus, surgical instrumentation, sonicator, BSL2 hood, dead box

Software-Gen5, Image Lab, Safetrace, Lifetrack, FacsCalibur, Anymaze, SPSS, Microsoft Office, Molis

Behavioral experience-open field, rotarod, light dark box, defensive burying, delayed alternation, home cage behaviors, myoclonic episodes, social interaction for the rat, social interaction for mice, water maze, radial arm maze, elevated plus maze, chronic unpredictable stress paradigm, novel object recognition, predator odor, conditioned fear, tail suspension, ladder, forced swim, feeding measurements and behavior

Work Experience:

USAISR, San Antonio, TX

August 2024-present

Sr. Biomedical Research Associate III

Performs coagulation tests on rodent plasma for prothrombin, fibrinogen, and activated partial thromboplastin time using Start4 instrumentation

Performs viscoelastic testing on rodent whole blood using ROTEM delta instrumentation

Validates dosages for various validations on new and novel drug or protein

Started training on BCA procedures and HPLC-MS extractions

MLM Medical Laboratories, Memphis, TN

August 2022-August 2024

Research Associate

Performed light transmission aggregometry and thrombin generation assays to determine drug effects on platelet function

Performed processes of DNA isolation and quantitation, polymerase chain reaction, and gel electrophoresis for restriction fragment length polymorphisms

Ran plate assays to determine drug effects on platelet function using Biotek Cytation 5 instrument

Created VASP ELISA and PLACT flow cytometry kits

Precision Life Sciences, Olive Branch, MS

January 2022- August 2022

Molecular Technologist

Performed respiratory pathogen panels, pharmacogenetic testing, urinary tract panels, and Covid testing using QuantStudio 12 technologies

Served as Safety Officer for laboratory

Aided in new testing validations

Capstone Healthcare, Atlanta, GA

January 2021-January 2022

Molecular Technologist Trainee

Performed inoculations to determine presence of urinary tract infections

Used polymerase chain reaction technologies for Covid-19 disease

Performed respiratory pathogen panels, pharmacogenetic testing, urinary tract panels, and covid testing using RT-PCR Quant Studio 12 technologies

LifeServe Blood Center, Des Moines, IA

April 2020-January 2021

Component Technician II

Created convalescent plasma treatments for Covid-19 patients

Performed quality control for safety and efficacy of blood products

Created red cell, apheresis and acrodose platelets, and plasma products

Versiti, Aurora, IL

August 2014-March 2020

Specialized Production Technician

• Performed moderate complexity testing for apheresis red cell and platelet products

• Executed lot release procedures

• Conducted quality control to ensure a safe and reliable blood product for the recipient

• Reviewed and maintained accurate records and documentation

• Revised Standard Operating Procedures

Component Technician

• Produced high quality blood products from whole blood as well as apheresis procedures

• Created leuko products for research

• Demonstrated leadership through example to encourage workflow efficiency

• Gained theoretical knowledge of Reference Laboratory procedures such as washings and freezing of rare units

Sample Management Laboratory Assistant

• Organized and processed forty-five thousand to sixty thousand samples weekly

• Analyzed pre-analytic, analytic, and post-analytic quality of samples

• Increased productivity and release of results up to two hours nightly to ensure client satisfaction

• Aided in validations and maintenance of refrigeration units, centrifuges, and decappers

Blood Collection Technician

• Performed phlebotomy per standard operating procedures

• Conducted mini physicals and interviews to ensure donor and recipient safety

• Aided in Supply Chain tasks such as cooler and mobile unit trunk creation

• Recruited donors for apheresis procedures up to seventy percent of donor pool compared to ten percent of company expectations

Indiana University Purdue University Indianapolis, Indianapolis, IN

June 2010-July 2013

Laboratory Technician

• Conducted numerous rodent behavioral assays for genetic and pharmaceutical research

• Managed rodent colonies for genotype, cost, and welfare

• Performed technical procedures including stereotaxic cannulations, mini-pump implantations, arterial catheterizations, ovarectomies, post-mortem tissue extractions, and histology

Florida State University, Tallahassee, FL

December 2007-October 2009

Laboratory Researcher

•Managed laboratory including cleaning, calibrations, validations, and ordering

•Used specialized equipment to determine biological basis of gustatory behaviors

•Trained new students on the use of specialized equipment, scientific principles, and laboratory safety

Northern Illinois University, Dekalb, IL

December 2002-December 2005

Laboratory Assistant/Student

Executed Chronic Unpredictable Stress Paradigm

Aided in rodent behavioral scoring and high-performance liquid chromatography components of studies

Created micro dialysis probes from raw materials as well as creation of educational materials for future students and employees

Washed laboratory glassware, and animal care

Publications:

Andrei I. Molosh, Philip L. Johnson, John P. Spence, David Arendt, Lauren M. Federici, Cristian Bernabe, Steven P. Janasik, Zaneer M. Segu, Rajesh Khanna, Chirayu Goswami, Weiguo Zhu, Su-Jung Park, Lang Li, Yehia S. Mechref, D. Wade Clapp, & Anantha Skekhar. Social learning and amygdala disruptions in Nf1 mice are rescued by blocking p21-activated kinase. Nature Neuroscience. 2014 Nov; 17 (11): 1583-1590.

Leslie Matuszewich, Jared J. Karney, Samantha R. Carter, Steven P. Janasik, Johanna L. O’Brien, & Ross D. Friedman. The delayed effects of chronic unpredictable stress on anxiety measures. Physiology & Behavior. 2007 Mar 16; 90(4): 674-681.

Acknowledgements:

Yada Treesukosol. Psyhophysical assessment of the role of the T1R2 and T1R3 protein subunits in taste responsiveness to polycose and putative T1R2+3 taste receptor ligands. Florida State University Libraries. 2010.

Yada Treesukosol, Clare M. Mathes, & Alan C. Spector. Citric acid and quinine share perceived chemosensory features making oral discrimination difficult in C57BL\6J mice. Chemical Senses. 2011 Jun; 36(5): 477-489.

Yada Treesukosol, Ginger D. Blonde, Enshe Jiang, Dani Gonzalez, James C. Smith, & Alan C. Spector. Necessity of the glossopharyngeal nerve in the maintenance of normal intake and ingestive bout size of corn oil by rats. Am J Physiol Regul Integr Comp Physiol. 2010 Oct; 299(4): R1050-R1058.

Yada Treesukosol, Ginger D. Blonde, & Alan C. Spector. T1R2 and T1R3 subunits are individually unnecessary for normal affective licking responses to polycose: implications for saccharide taste receptors in mice. Am J Physiol Regul Integr Comp Physiol. 2009 Apr; 296(4): R855-R865.

Seminars/Webinars:

Shearly you can’t be serious! T-TAS® 01 for Total Platelet Function Testing, February 2023. Diapharma.

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