Medical Technologist Clinical Laboratory

FARZANA S. SIDDIQUI

**** ******** ****

Silver Spring, Maryland, 20904

Home: 301-***-****

Cell: 443-***-****

OBJECTIVE A technical position utilizing my skills, knowledge, and experience in clinical laboratory PROFESSIONAL

EXPERIENCE

10/2005 – present CHILDREN’S NATIONAL MEDICAL CENTER Washington DC Medical Technologist, Molecular/ PCR

Perform DNA extraction by QIA Symphony Robot; Quantitative analysis of CMV and EBV using Smart cyclers; Perform quantitative analysis of HIV VL using the ROCHE COBAS AMPLIPREP; Qualitative analysis of HIV TNA using ROTARGENE; Perform HSV PCR Qualitative detection on Smart cyclers; Qualitative detection of ADENOVIRUS PCR using ABBOT M2000/ ROTARGENE; Perform HEA PCR using Thermo cyclers; CT/NG Qualitative Detection using ABBOT M2000; Perform Chromosomal Microarray using GENE DX ROBOTS AND SCANNER to detect genetic disorders; Detect genetic disorders using NEXTGEN ; Perform SANGERS PCR confirmation test for NEXTGEN ; Carry out RUU PCR confirmation for NEXTGEN/CMA using 15 different Snypses; Perform Real Time PCR on Thermo cyclers; GENMARK/PRODESE/LUMINEX; Perform CYSTIC FIBROSIS PCR using LUMINEX; Perform Fragile X GENE test to detect three base pair repeat disorders using 3100/ 3500 Sequencers; detect Factors II and V, MRSA using Gene Xpert

8/2001 – 10/2005 J. Craig Venter Science Foundation (JTC) Rockville, Maryland Biotechnologist II, DNA Sequencing Department

Isolate plasmid and genomic DNA using Shotgun sequencing techniques via Dye terminator, PCR optimization cloning and restriction enzyme digestion; Operate ABI prism 3700, 3730XL, and 3100; perform PCR re-sequencing, and BAC end reactions; operate and troubleshoot Thermal cyclers, Biomek FX, and PIXSYS 8/1999 – 8/2001 Johns Hopkins Hospital Baltimore, Maryland Laboratory Technician, Clinical Immunology

Perform RPR; order inventory; Process specimens

10/1997 – 8/1999 DNA Technologies, Inc. Gaithersburg, Maryland Research Assistant

SKILLS Sunquest, various databases, Excel, Microsoft Windows, Internet EDUCATION Advanced Education/ Post-graduate training FAES Graduate school NIH Bethesda, Maryland 2002

University of Karachi Pakistan

Bachelor of Science, Biochemistry 1994

STATUS U.S. Citizen

REFRENCE Available upon request

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