Co-Op 3D Printing

Taylor Smola

860-***-**** ********@*****.*** Linkedin Boston, MA

Biological engineer with 1+ years of experience in a fast-pacedresearch lab environment. Designed microfabricated devices incorporating concepts from both biology and engineering. Established four human cell lines simultaneously both in routine culture and experimentation. Developed unique tissue models and microfluidic devices using online design platforms and 3D printing. Able to distill complex ideas for a diverse audience when presenting the end of co-op/capstone research materials. EDUCATION

Wentworth Institute of Technology (WIT) Boston, MA Expected Grad: August 2025 Bachelor of Science in Biological Engineering, Minor In Chemistry GPA : 3.80 TECHNICAL SKILLS

Laboratory Techniques: Mammalian Cell Culture, Transfection, Cellular Imaging using Fluorescence Microscopy, Microfabrication of Cellular Devices, Soft Lithography, Flow Cytometry, Cellular Assays, PCR, Gel Electrophoresis including: Agarose gels, and SDS-PAGE, Western Blotting, Electronic Prototyping, Plasmid Preparation, DNA isolation, RNA isolation,Thin layer Chromatography, Micropipetting, Centrifugation, Bradford Assay, Spectrophotometry, Biofuel Enzyme Assays, Bacterial Cell Culture, Bacterial Transformation, Column chromatography including HIC, SEC, and Ion exchange columns, Gas Chromatography, Protein purification and characterization, Affinity purification. Programming Language: MATLAB, Arduino

Software: TinkerCad, 2021 Solidworks, GraphPad Prism, Microsoft Office, Word, Excel WORK EXPERIENCE

Synthetic Biology Co-op Wentworth Institute of Technology Boston, MA September 2024 - December 2024

● Created spheroids at high-throughput while varying bioinks and cell types to discover ideal 3D culture conditions

● Developed microfabricated devices to analyze SIM-A9 cellular spheroids under constant flow conditions

● Transfected CHO cells to create new cell lines to use in spheroid experimentation Independent Study Wentworth Institute of Technology Boston, MA January 2024 - September 2024

● Building cell-based sensing for organoid cultures, in particular cardiomyocytes

● Designing and fabricating micro-devices for studying mitochondrial localization in mammalian cells

● Implementing live-cell microscopy experiments for cellular differentiation assays Biology Laboratory Technician Co-op Wentworth Institute of Technology Boston, MA January 2024 - April 2024

● Mentored students and adapted protocols in a fast-paced lab environment teaching molecular biology

● Tasks included, cell culturing, DNA isolation, drosophila husbandry, hazardous waste disposal, chemical inventory and storage, solution preparation, agarose gel preparation, bacterial culture plate preparation, EHS certification

● Explored significance of the Tau protein in Alzheimer’s Disease and authored a comprehensive literature review incorporating contemporary research findings on the topic RESEARCH AND LAB WORK

Fabrication of High Throughput Stem Cell Culture and Purification Device January 2025 - Present

● Created a protocol to upscale stem cell culture production using microcarriers in a bioreactor

● Attached a magnetic tag to enrich for stem cells

● Created a closed cartridge system that sorts stem cells based on their magnetic tags Development of Neurospheres - Synthetic Biology Co-op September 2024 - December 2024

● Created 3D printed 96-well plate holder to ensure sterility throughout spheroid fabrication procedure

● Trialed various cell inks using SIM-A9 microglial cells, Sodium Alginate, and Rat Collagen 1

● Created and molded a spheroid chip with PDMS to analyze individual spheroids under constant flow conditions Creation of an Artificial Tissue - Biomaterial and Tissue Engineering May 2024 - August 2024

● Building hydrogels in a variety of shapes and sizes

● Using cell-printing technologies (i.e., CellInk) to make organotypic cultures

● Assembling and studying cardiosphere cultures comprised of human cardiomyocytes Creation of a Transgenic Plant - Genetics and Transgenics Course September 2023 - December 2023

● Prepared a genetically modified Agrobacterium with a CMV35s Ruby promoter

● Transfected Agrobacterium tumefaciens using Mag-Bind Particles in magnetic separation to extract purified plasmid

● Using microparticle bombardment we scratched off surface of plant leave and injected CMV35s plasmid into plant

● Plant presented with red dots around the injection site proving success of transformation ACTIVITIES AND ACCOMPLISHMENTS

Academic Accomplishments September 2021 - Present

Wentworth Institute of Technology

Leading Leopard Award, Presidents Award, Dean's list Awards presented for leadership in a club, contributions to the community, and high academic achievement American Society of Biochemistry and Molecular Biology, Wentworth September 2022 - Present Secretary, Chief of Science

Presented scientific articles to ASBMB club for various disease awareness months Alzheimer’s Disease Research Center (BU ADRC) Student Ambassador Program, Boston University 2023 - 2024 Student Ambassador

Advocated for Alzheimer’s Disease prevention and treatment options within the Boston community Martial Arts 2014-Present

Jae Hun Kim Tae Kwon Do Institute - 2nd Degree Black Belt World Tang Soo Do Association - 2nd Degree Black Belt

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