Research Associate Molecular Biology

Sri Haindhavi

Dallas, TX ************@*****.***

Profile

Intend to build a career where I can explore my knowledge fully and realize my potential. Having 5 years of experience in Molecular Biology and would like to associate with an organization, that gives me scope to apply my knowledge and skills that pursues a challenging and group-oriented career. Technical exposure

MOLECULAR BIOLOGY

· TECHNIQUES: DNA and RNA extraction, PCR, RT-PCR, Library preparation for NGS. · PLATFORMS: ABI 7500 Fast dx, Rotor Gene RGQ, Bio- Rad CFX96 thermal cyclers, GeneXpert, Thermofisher; Kingfisher FLEX and QIA Cube automated extraction system and, and Ion torrent S5 for Next gen sequencing. FLOW CYTOMETRY

· cd3 and cd4 count from whole blood samples, Hla B 27 counting, and stem cells enumerations in BD facsmachine.

CYTOGENETIC

· Cell culturing, harvesting, human chromosomal lied preparation, banding, Banded karyotyping for blood, Bone marrow, POC (Product of conceptions), RPMI media preparation. Work Experience

MOLECULAR SCIENTIFIC OFFICER APOLLO DIAGNOSTICS – GLOBAL REFERENCE LAB, HYDERABAD, INDIA SEPTEMBER 2020 TO MARCH 2023 · Hands on experience of Next gen sequencing; manual library preparation and chip loading. · Work experience on infectious test handling like

(HIV, HCV, HBV, HSV, CMV, HPV, MTB and COVID-19). · SNP detection and genotyping (e.g.: HLA), Onco-marker detection like BCR-ABL and JAK2 based on RT PCR.

· Validations and establishment of new RT-PCR based assays.

· Experience in laboratories recognized with NABL and CAP organizations, well-versed with audits and documentation (QC documents, proficiency testing, maintenance records, SOP preparation, log, and safety policy maintenance).

· Managed team during covid pandemic in RT-PCR tests.

· Training new joiners in the present lab testing procedures and equipment. JUNIOR SCIENTIFIC OFFICER BIOGNOSYS, HYDERABAD, INDIA AUGUST 2019 TO SEPTEMBER 2020

· Handled Infectious samples that include HIV, HCV, HBV, and MTB.

· Hands-on experience with RT-PCR

· DNA/RNA Isolation using available Kits and conventional in-house protocols. Education

MASTER OF SCIENCE IN BIOTECHNOLOGY JUNE 2019 OSMANIA UNIVERSITY, HYDERABAD, INDIA

BACHELOR OF SCIENCE IN BIOTECHNOLOGY AND FORENSIC JUNE 2017 OSMANIA UNIVERSITY

Certifications & Conferences

· Participated in virtual training in “Internal Audit and Quality Management System as per ISO 15189:2012 & NABL 112 Certificate Course” from 2 – 5 October 2020.

· Certified in “Hands-on Training Program on Rotor Gene – Q” conducted by QIAGEN. Internship Programs

INTERN TELANGANA STATE FORENSIC SCIENCE LABORATORY, INDIA JULY 2018 TO OCTOBER 2018

· Preparing buffers and extracting DNA for forensic analysis · Assuring experimental and safety protocols, analyzing results, and creating reports Project Title: Resolving murder cases and disputed identity cases using DNA Profiling involving DNA Isolation, PCR analysis, and Y-chromosome STR Analysis techniques. References

· Dr. Eswari Dodagatta-Marri PhD at Brunel University, London, UK, Post-Doctoral Scholar at UCSF, San Francisco, California, USA Email: **********@*****.*** Mobile: +918*********

· Dr. Sireesha Vaidya PhD (Genetics) at Osmania University, Hyderabad, India Email: ***********@*****.*** Mobile: +919*********

· Dr. Thishya Kalluri PhD at Nizam Institute of Medical Sciences, Hyderabad, India Email: ********@*****.*** Mobile: 917-***-****

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