Information Technology Health Care

NING HUANG

Mobile: 919-***-**** Email: ************@*****.*** Location: Chapel Hill, NC 27516

PROFESSIONAL SUMMARY

Talented Biology Instructor offering expertise in building future experts in Biology field with thought-provoking instruction. Expand student knowledge through lectures, group projects, and lively discussions. Stay at forefront of field with continuous professional development.

Smart and insightful Research Fellow specializing in Microbiology. Successful at planning, executing and analyzing research. Brings PhD and experience in peer-reviewed publication. Always looking for ways to improve studies and validity of results.

SKILLS

• Bacterial/Cell/Raft Culture • RNA/DNA/Protein Extraction • RT-qPCR/LAMP

• Western/Southern/Northern Blot • Transmission electron microscopy • Immunofluorescence assay

• Co-immunoprecipitation • In Situ Hybridization • RNA silencing

• DNA Cloning/Sequencing • Site Directed Mutagenesis • Immunohistochemistry

• Flow cytometry • ELISA/ELFA • Advanced Data Analysis EXPERIENCE

Postdoctoral Researcher

Cary Moody Chapel Hill, NC

February 2019 - July 2022

• Understanding the mechanisms on how HPV utilizes caspase activity to block an IFN response.

• Researched and collected research data and maintained daily records of methodologies and test results.

• Interpreted experiment and test results using statistics and hypothesis testing.

• Prepared manuscripts for submission to academic journals and conferences.

• Assisted in preparation and submission of research grants, scientific papers, peer-reviewed articles and research- related correspondence.

• Supervised graduate students working on thesis projects within same area of expertise.

• Trained undergraduate students in laboratory techniques and data analysis methods.

• Kept laboratory and field equipment in good working condition. Postdoctoral Research Fellow

Andrew Mercer Dunedin, Otago, New Zealand

June 2016 - August 2018

• Investigate the mechanism of ORFV counteracts with BAF by phosphorylating BAF during ORFV infection.

• Completed and summarized statistical analyses of research results and prepared forms to present information.

• Identified problems and performed analysis and troubleshooting to refine procedures.

• Presented research findings at conferences, workshops, or other professional events.

• Encouraged graduate students to investigate further and develop expertise through individual and group projects. Research Associate

Rollie Clem Manhattan, KS

February 2012 - May 2015

• Understanding the molecular mechanisms of arbovirus midgut escape.

• Investigated the roles of effector caspases in mosquito midgut basal lamina remodeling during arbovirus infection.

• Supervised graduate students working on thesis projects within same area of expertise.

• Trained undergraduate students in laboratory techniques and data analysis methods.

• Prepared manuscripts for submission to academic journals and conferences. Biology Teacher

Sanshui Oversea High School Foshan, Guangdong, China August 2002 - July 2006

• Developed creative lesson plans in accordance with school curriculum, state standards and field advancements.

• Taught students fundamentals and advanced Biology concepts through lectures and assignments.

• Evaluated student performance and achievement of learning objectives, leveraging findings to enhance course content.

• Assessed student progress through quizzes, tests, and assignments.

• Supervised laboratory work, promoting safety and proper equipment handling to mitigate risk and protect equipment.

• Managed student and parent expectations by clearly outlining objectives and requirements.

• Provided extra support for struggling students through after-school tutoring sessions.

• Mentored new teachers by sharing expertise and providing guidance throughout their first year of teaching.

• Participated in professional development workshops to stay current on best practices in biology education. EDUCATION

Bachelor of Science (B.S.) - Biology Education

Zhanjiang Normal College, Foshan City, Guangdong Province, China May 2002

Doctor of Science (D.Sc.) - Microbiology

Sun Yet-sen University, Guangzhou, China

December 2011

• Studied abroad at Kansas State University as an exchanged student and conducted PhD research projects from 2009 to 2011.

• Obtained Scholarship from China Scholarship Council. Diploma - Biblical Languages Study

University of Otago, Dunedin, New Zealand

December 2018

Certification - Modern Hebrew

Ben Gurion University of the Negev, Be'er Sheva, Israel February 2019

LANGUAGE

Chinese

Native (Mandarin and Cantonese)

English

Fluent

Spanish

Conversational

PUBLICATIONS

• Huang N, Clem RJ, Rohrmann GF. 2011. Characterization of cDNAs encoding p53 of Bombyx mori and Spodoptera frugiperda. Insect Biochemistry and Molecular Biology 41:613–9.

• Huang N, Wu W, Yang K, Passarelli AL, Rohrmann GF, Clem RJ. 2011. Baculovirus infection induces a DNA damage response that is required for efficient viral replication. Journal of Virology 85:12547-56.

• Brand IL, Green MM, Civciristov S, Pantaki-Eimany D, George C, Gort TR, Huang N, Clem RJ, Hawkins CJ. 2011. Functional and biochemical characterization of the baculovirus caspase inhibitor MaviP35. Cell death disease 2:e242.

• Huang N, Civciristov S, Hawkins CJ, Clem RJ. 2013. SfDronc, an initiator caspase involved in apoptosis in the fall armyworm Spodoptera frugiperda. Insect biochemistry and molecular biology 43:444–54.

• Katelyn O'Neill, Bradley J.S.C. Olson, Ning Huang, and Rollie J. Clem. Strong selective pressure against a recombinant Sindbis virus that induces apoptosis in the mosquito Aedes aegypti. Proc Natl Acad Sci U S A. 2015 Mar 10; 112(10): E1152–E1161.

• Ning Huang, A. Lorena Passarelli, and Rollie J. Clem. A Potential Role for effector caspases CASPS18 and CASPS19 in midgut escape of Sindbis virus in Aedes aegypti. ( In preparation)

• Ning Huang, Alexander W.E. Franz, A. Lorena Passarelli, and Rollie J. Clem. Regulation and activation of two effector caspases that affect Sindbis virus replication in mosquitoes. ( In preparation)

• Ning Huang, Jin Lee and Andrew Mercer. BAF phosphorylation through LEM regualtion during orf virus infection.

(In preparation)

• Huang N, Des'ree G, Blossom Damania and Cary Moody. Apoptotic caspases suppress an MDA5-driven IFN response during productive replication of human papillomavirus type 31. Proc Natl Acad Sci U S A. 2022 Jul 19;119(29):e2200206119.

• Huang N, Studstill C, Sundstrom S, Moscoso S, Zhang H, Damania Blossom and Moody Cary. Apoptotic Caspases Suppress Expression of Endogenous Retroviruses in HPV31+ Cells That Are Associated with Activation of an Innate Immune Response. Viruses 2024, 16(11), 1695; https://doi.org/10.3390/v16111695. CONFERENCE PRESENTATIONS

• Huang N and Cary M. Huang N. Apoptotic caspases suppress an MDA5-driven IFN response during productive replication of human papillomavirus type 31. 40th Annual Meeting of the American Society for Virology, July 2021, Virtual Meeting.

• Huang N, Jin L and Andrew M. BAF is phosphorated during orf virus. XXI International Poxvirus Asfarvirus and Iridovirus Conference, July 2016, Strausburg, France.

• Huang N, Passarelli AL, and Clem RJ. A potential role for CASPS18 and CASPS19 in midgut escape of Sindbis virus in Aedes aegypti. 13rd Annual Symposium in Virology, October 2013, Lincoln, Nebraska, USA.

• Huang N, Passarelli AL, and Clem RJ. A potential role for CASPS18 and CASPS19 in midgut escape of Sindbis virus in Aedes aegypti. 32nd Annual Meeting of the American Society for Virology, July 2013, State College, Pennsylvania, USA.

• Huang N, Civciristov S, Hawkins CJ, Clem RJ. Identification and characterization of the initiator caspase SfDronc in Spodoptera frugiperda and its role in apoptosis induced by Autographa californica M nucleopolyhedrovirus. 45th Society for Invertebrate Pathology Annual Meeting, August 2012, Buenos Aires, Argentina.

• Wu W, Huang N, Clem RJ, Rohrmann GF, and Passarelli AL. Characterization of the interaction between the AcMNPV sulfhydryl oxidase Ac92 and the Spodoptera frugiperda P53 protein. 45th Society for Invertebrate Pathology Annual Meeting, August 2012, Buenos Aires, Argentina.

• Huang N and Clem RJ. Identification and characterization of the initiator caspase SfDronc in Spodoptera frugiperda and its role in apoptosis induced by Autographa californica M nucleopolyhedrovirus. 31st Annual Meeting of the American Society for Virology, July 2012, Madison, Wisconsin, USA.

• Wu W, Huang N, Clem RJ, Rohrmann GF, and Passarelli AL. Characterization of the interaction between the AcMNPV sulfhydryl oxidase Ac92 and the Spodoptera frugiperda P53 protein. 31st Annual Meeting of the American Society for Virology, July 2012, Madison, Wisconsin, USA.

• Huang N, Wu W, Passarelli AL, Rohrmann GF and Clem RJ. Understanding the roles of p53 and the DNA damage response in baculovirus replication. 44th Society for Invertebrate Pathology Annual Meeting, August 2011, Halifax, Nova Scotia, Canada.

• Huang N, Wu W, Passarelli AL, Rohrmann GF and Clem RJ. Identification of lepidopteran homologs of the tumor suppressor gene p53 and interaction of Spodoptera frugiperda p53 with the baculovirus protein Ac92. 30th Annual Meeting of the American Society for Virology, July 2011, Minneapolis, MN. LOCAL PRESENTATIONS

• Characterization of Spodoptera frugiperda homolog of p53 and understanding its roles in baculovirus replication ---

--- Apr. 25, 2011. Molecular, Cellular and Developmental Biology Program Research Forum, Kansas State University.

INTERNATIONAL PRESENTATIONS

• The roles of DNA damage response and SfP53 in relation to replication of baculovirus and apoptosis induced by baculovirus Nov, 2011. Sun Yet-sen University, Guangzhou, P. R. China. COMMUNITY SERVICE

Volunteer and other EXPERIENCE

• Volunteer Treasurer and activist planner in Christian Witness Fellowship non-profit organization Feb. 2019 - Present

• Vocation Bible School Volunteer in Chinese Christian Mission Church Jul. 2022-Present

• Volunteer in 4H STEM Summer Camp Jul. 2023-Present

• George Street Elementary School Classroom parent helper Aug. 2018 - Dec. 2018

• Salsa dance Woman team and coach assistant in Dunedin Salsa Club Jan. 2017 - Jul. 2018

• Boys & Girls Club of Manhattan, Kansas, USA.

Coach Assistant in 1st Grade Boys' basketball team Jan. 2011 - Mar. 2011

• Sanshui Overseas Senior High School, Foshan, China Coach Assistant in High School Girls' basketball team Aug. 2003 - Jun. 2004

• Zhanjiang Normal College, Zhanjiang, China.

Captain of women's basketball team in Biology Department Sep. 1998 - Jun. 2002

• Zhanjiang Normal College, Zhanjiang, China.

Captain of College women's basketball team Sep. 2000 - Jun. 2002

• Zhanjiang Normal College, Zhanjiang, China.

President of Student Ballroom Dance Association Sep. 1999 - Jun. 2001 AWARDS AND HONORS

• 2017 2nd place in Amateur Salsa Women Team in 2017 South Island Latin Competition

• 2016 Travel Award, XXI International Poxvirus Asfarvirus and Iridovirus Conference

• 2013 Travel Award, Johnson Basic Cancer Research Center ($600)

• 2011 Student Travel Award, American Society Virology ($500)

• 2004 Outstanding Young Teacher Award, Bureau of Education of Guangdong

• 2002 Outstanding Sportsmanship Award as a women basketball player, Zhanjiang Normal College

• 2001 Outstanding Sportsmanship Award as a women basketball player, Zhanjiang Normal College

• 2001 President's Award for Outstanding Student Leadership, Zhanjiang Normal College

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