Molecular Biology Quality Control

FORAM PATEL

Boston - MA 781-***-**** *************@*****.*** https://www.linkedin.com/in/foram-patel-0257682a1/

Education

Master of Science, Biotechnology 05/ 2025

Northeastern University, Boston, MA

Bachelor of Science, Biotechnology 05/2023

Charotar University of Science and Technology, Anand, Gujarat

Relevant Coursework: Genetic engineering, Genetics, Cellular and Molecular Biology, Immunology, Medical biotechnology, Animal Biotechnology, Developmental biology, Industrial Biotechnology, Omics, and synthetic biology, Cell culture in biopharmaceuticals, Biostatistics, Protein Principles in Biotechnology, Nanomedicine Research Techniques, Biotechnology Applications Laboratory, Foundations in Nanodiagnostics, Analytical Techniques

Skills

Lab Skills - DNA & RNA isolation, PCR, SDS-PAGE, production, surface modification and characterization of nanoparticles, Liposome synthesis – Thin film hydration, Microfludics, pGLO transformation - bacterial transformation, CRISPR-Cas9 genome editing, MTS Assay, Clonogenic Assay, BCA Assay, Sanger sequencing, western blotting, pipetting, nanodrop, immunohistochemistry, immunocytochemistry, agarose gel electrophoresis, ELISA, cell culture, transfection, molecular cloning, colony PCR, minipreps, glycerol stocks, gel extraction, HIC, Lentivirus transduction, tapestation

Equipment – Nanoassemblr, Fluorescence microscopy, UV spectrophotometer, Thermocycler, AKTA, Centrifuge, vacuum manifold

Software skills – Microsoft Excel, Powerpoint, Word, Outlook, Teams

Work Experience

Intern at Sterling Accuris Laboratory, India 06/2022 – 12/2022

Worked in an aseptic environment while performing urine analysis, specializing in protein and glucose testing for diagnostic purposes

Strictly adhered to microbiological protocols while performing malarial parasite count using Giemsa staining

Conducted accurate and efficient blood group testing using anti – serum, ensuring compatibility for safe blood transfusions

Executed serum and plasma separation techniques, maintaining the integrity of diagnostic sample

Implemented and adhered to strict quality control procedures, contributing to reliable and error-free laboratory results

[Harvard Medical School and Women’s Brigham Hospital / Sherwood Lab]

Molecular Biology Research Project: Investigation of IGSF23 and TRIQK Genes in Cholesterol secretion 01/25 - Present

Engaged in molecular cloning and CRISPR-based gene editing to investigate the roles of IGSF23 and TRIQK genes in cholesterol secretion, with an emphasis on understanding gene function through base editing

Utilized Adenine Base Editors and Cytosine Base Editors to perform targeted base edits on specific genes

Conducted cloning of gRNA into CRISPRv2 vectors (p275 and p839) and performed precise digestion using BsmBI and Esp3I restriction enzymes to prepare gene fragments for further analysis

Executed gel extraction procedures to isolate the desired 13,785 bp and 14,313 bp fragments after enzyme digestion, ensuring high-quality DNA for subsequent assembly

Executed NEBuilder HiFi DNA assembly reactions to create precise plasmid constructs incorporating the base editors, ensuring successful DNA assembly through thermocycling and transformation into competent E. coli cells

Performed bacterial transformations using NEB® Stable Competent E. coli cells, followed by colony picking and miniprep for subsequent sequencing to confirm the accuracy of the cloned constructs

Evaluation of StitchR for 3-way Trans-Splicing in Mammalian Cells 04/25 - Present

Designed and executed a molecular cloning strategy to construct plasmids for testing 3-way trans-splicing using StitchR, including PCR amplification, gel purification, and NEBuilder assembly

Performed transformation into competent bacterial cells, colony screening via restriction digest, and sequence verification to confirm correct constructs

Optimized transfection protocols for HCT116 cells using multi-plasmid delivery and standardized normalization approaches for experimental and control conditions

Isolated high-quality RNA from transfected cells and performed cDNA synthesis using NEB Protoscript II, followed by downstream PCR analysis

Developed and validated PCR assays to distinguish between 2-way and 3-way stitched transcripts, enabling robust detection of trans-splicing events

Conducted TapeStation analysis for quantitative and qualitative assessment of PCR products, confirming the presence and ratio of 2-way (121–127 bp) and 3-way (168–174 bp) stitched transcripts in experimental replicates

Demonstrated successful 2-way trans-splicing in control samples and detected both 2-way and 3-way stitched transcripts in experimental samples, supporting the feasibility of multiplexed RNA splicing using StitchR

Academic Projects Northeastern University

Developed a CD28 - Directed CAR T Cell Therapy for the Treatment of Cancer 01/2023 – 04/2023

Conducted theoretical research and analysis on acute lymphoblastic leukemia and CAR T-cell therapies

Designed hypothetical preclinical studies using animal models

Outlined a hypothetical clinical trial plan from Phase 0 through Phase 4

Proposed a hypothetical manufacturing process for CAR T-cell production

Demonstrated ability to communicate complex scientific concepts through presentation

Exhibited teamwork and collaboration skills in a hypothetical project setting

Comprehensive Exploration of GFP Expression, Purification, and Characterization in Escherichia coli 01/2024 – 04/2024

Study focused on the process of transforming Escherichia coli with the Green Fluorescent Protein (GFP) gene

Steps included meticulous selection, growth, and lysis of the bacterial culture

Utilized techniques such as Ponceau gel staining, Western blot analysis, drip column chromatography, and the AKTA pure system for GFP purification

Ensured integrity and purity of the GFP protein

Aimed to characterize GFP's behavior under varied temperature and pH conditions

Provided comprehensive insights into GFP's fluorescent properties

Established a robust protocol for GFP expression and purification

Contributed valuable data and methodologies for researchers exploring GFP's applications in molecular biology, biotechnology, and related fields

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