TARANEH GHAROONI
****************@*****.*** San Jose, CA 925-***-**** U.S. CiƟzen
linkedin.com/in/taraneh-gharooni-78374277/
SCIENTIST
Professional Scientist with expertise in LCR, LDR, RPA, LAMP, PCR, qPCR, ddPCR, RT-PCR, DNA sequencing (Next- Generation Sequencing using Illumina MiSeq). Extensive experience in plant and mammalian cell culture, DNA/RNA extraction, and spectrophotometry. Strong knowledge of GMP/GLP regulations, handling clinical samples, and working in BSL-2 environments. Core competencies include: Research & Development (R&D) Problem-Solving Data Analysis Publishing Compliance Safety PROFESSIONAL EXPERIENCE
Stealth mode Startup., Sunnyvale, CA 3/2025_Present Startup focused on molecular diagnosƟcs for the detecƟon of geneƟc diseases. ScienƟst
Independently design, develop, and validate LCR and LDR assays.
Troubleshoot technical issues and assay optimization.
Present experimental data, conclusions, and recommendations to key stakeholders and contribute to data-driven decision-making.
Perform other duties as assigned.
Cepheid Inc., Sunnyvale, CA 2/2022 – 3/2025
Molecular diagnosƟcs co. dedicated to improving healthcare through innovaƟve molecular systems and tests. ScienƟst II
Designed and opƟmized molecular assays for detecƟng infecƟous diseases using real-Ɵme PCR technology.
Collaborated with cross-funcƟonal teams to support product development and commercializaƟon.
Analyzed and interpreted assay data using staƟsƟcal methods, presenƟng results to management and external partners.
Led a team of research associates, providing technical guidance and mentorship.
Designed, executed, and documented experimental results.
Authored and validated SOPs and reports for the Assay Development team. InSilixa Inc., Sunnyvale, CA 5/2017 – 1/2022
Startup focused on mulƟplexed DNA tesƟng for disease detecƟon & anƟbioƟc resistance using CMOS technology. Senior Research Associate (1/2020 – 1/2022)
Analyzed data using staƟsƟcal tools (MicrosoŌ Excel, QuantaSoŌ Analysis Pro) and presented findings at technical meeƟngs.
Designed and developed control experiments.
OpƟmized sample preparaƟon and performed RNA/DNA extracƟon and quanƟficaƟon.
Conducted PCR, qPCR, ddPCR, RT-PCR, and performed data analysis.
Managed, organized, and supported lab operaƟons.
Designed, developed, and opƟmized experimental protocols to enhance assay performance. TARANEH GHAROONI 925-***-**** Page2
Research Associate (8/2017 – 1/2020)
Conducted RNA/DNA extracƟon, quanƟficaƟon, and analysis.
Performed PCR, qPCR, ddPCR, RT-PCR, and data analysis.
Managed lab operaƟons and developed experimental protocols.
Prepared primers and probes, ensuring quality and concentraƟon standards. Intern Researcher (5/2017 – 8/2017)
Performed DNA/RNA extracƟon, PCR, and qPCR. Organized data in Excel for staƟsƟcal analysis. Clear Labs Inc., Menlo Park, CA 1/2016 – 8/2016
Startup specializing in molecular-level food analysis using DNA barcoding. Intern Researcher
Received and logged samples using the company’s LIMS system.
Conducted food sampling, DNA extracƟon, and PCR procedures.
Performed Next-GeneraƟon Sequencing (NGS) using Illumina MiSeq, opƟmizing protocols for improved efficiency.
Agricultural Biotechnology Research InsƟtute of Iran (ABRII), Karaj, Iran 7/2010 – 10/2013 ABRII specializes in plant molecular biology and biotechnology with a team of ~100 researchers. Research Assistant
Conducted Ɵssue culture and micro-propagaƟon of tomato (Lycopersicon esculentum) and cucumber
(Cucumis saƟvus).
Prepared, sterilized, and dispensed culture media.
Maintained sanitaƟon protocols in asepƟc areas.
EDUCATION CERTIFICATION
Bridge to Bioscience Lab Assistant CerƟficate Program, City College of San Francisco Master of Science (MS) in Plant Biotechnology & Molecular Technology of HorƟculture Crops University of Tehran, Tehran, Iran
Bachelor of Science (BS) in HorƟculture Sciences
University of Tehran, Tehran, Iran
TOOLS TECHNOLOGIES
Microsoft (Word + Excel + PowerPoint) Minitab QuantaSoft Analysis Pro Freezerworks PUBLICATIONS
Hassibi, A., Ebert, J., Bolouki, S., Anemogiannis, A., Mazarei, G., Li, Y., Johnson, K.A., ManƟna, P., & Gharooni, T., (2018). An array-based melt curve analysis method for the idenƟficaƟon and classificaƟon of closely related pathogen strains. Biology Methods & Protocols, 3(1).
Gharooni, T., Zamani, Z., & Bouzari, N. (2012). GeneƟc variaƟon of Mahaleb (Prunus mahaleb L.) genotypes based on morphological traits and RAPD primers. Seed and Plant Improvement Journal, 28(1), 4.
Gharooni, T., (2008). Comparing ploidy levels in 8 genotypes of Mahaleb seed trees. Second InternaƟonal Student Conference of Biotechnology, University of Tehran, 15–17 November 2008.