Research Scientist Assistant

Timothy Macatee

**********@*****.*** • +1-201-***-**** • Shrewsbury, MA • https://www.linkedin.com/in/timothy-macatee/

SUMMARY

Experienced research scientist with a strong background in laboratory procedures, molecular biology and bioanalysis. Proficient in DNA/RNA extraction, immunohistochemistry, Standard Operating Procedures (SOPs), and GLP practices. Skilled in using analytical instrumentation software and standard laboratory data and calculations. Eager to contribute to your team by leveraging technical skills and innovative solutions.

EXPERIENCE

UMass Chan Medical School, Senior Research Scientist Feb 2022 - Apr 2025

•Investigated tropism of HIV viruses in children, using production of envelope gene pseudo-viruses and testing the ability to infect different cell types, enhancing understanding of viral behavior.

•Measured viral load of EBV in plasma using qPCR, ensuring accurate and reliable bioanalytical results.

•Assessed immune responses using the O-link platform, contributing to the advancement of immunological research.

MD Anderson Cancer Center, Research Assistant II Dec 2020 - Feb 2022

•Conducted RT-QPCR, sequencing, and cloning of virus-positive samples, ensuring accurate identification and analysis of viral presence.

•Performed lab testing on baboon pathology samples to establish a Specific Pathogen Free baboon colony, enhancing research quality and reliability.

•Utilized analytical instrumentation software and standard laboratory calculations, improving data accuracy and laboratory efficiency.

MD Anderson Cancer Center, Research Investigator Apr 2013 - Nov 2020

•Investigated the role of epigenetic changes in mice and cell culture, contributing to the understanding of cellular changes and cancer progression through advanced techniques.

•Conducted immunohistochemistry, confocal imaging, cell culture, immune-precipitation, RT-QPCR, and Western Blot analysis, ensuring high-quality and reliable results.

•Utilized analytical instrumentation software and standard laboratory calculations, enhancing the accuracy and efficiency of bioanalytical research.

University of Pennsylvania, Research Specialist B Jul 2011 - Apr 2013

•Developed SOPs and GLP practices for the laboratory, ensuring compliance with regulatory standards and improving operational efficiency.

•Created and maintained a comprehensive database for the receipt and storage of pre-clinical samples, enhancing data management and accessibility.

•Performed flow cytometry assays on CarT-19 patient samples, contributing to the advancement of translational research and improving assay accuracy.

Children's Hospital of Philadelphia, Research Associate II Nov 2010 - Jul 2011

•Conducted research on targeted gene deletion in cardiac and vascular development, utilizing histology of mouse embryos and whole mount in-situ hybridization.

•Performed PCR genotyping and gene cloning, ensuring accurate genetic analysis and contributing to developmental biology research.

•Handled small animals and conducted microphotography, enhancing the quality of research data and visual documentation.

NYU School of Medicine, Senior Research Technician Jan 2005 - Nov 2010

•Supervised and managed the Research Histology Core Facility, enhancing operational efficiency through meticulous maintenance and upkeep of all core facility equipment.

•Trained and mentored post-docs and students in histology and microscopy techniques, fostering a collaborative and educational environment.

•Conducted advanced tissue processing, sectioning, and staining, as well as bright field and fluorescent macro and microphotography, ensuring high-quality results for research projects.

University of Utah, Research Assistant II Nov 2002 - Nov 2004

•Conducted research on mouse heart development, focusing on germ layer-specific knock-out of the FGF8 gene, identifying gross anatomical changes.

•Utilized advanced techniques in gene knock-out studies, contributing to the understanding of developmental biology.

•Collaborated with a multidisciplinary team to analyze and interpret data, ensuring accurate and reliable results.

EDUCATION

University of Houston

Bachelor of Science, Marine Biology

Fairleigh Dickinson University Master’s work, Marine Biology

PUBLICATIONS

https://www.ncbi.nlm.nih.gov/pmc/?term=Macatee+T

SKILLS

DNA • FPLC • RNA • Extraction • Microscopy • Western Blot • Immunohistochemistry • High-Performance Liquid Chromatography • GLP • Spectroscopy • Laboratory procedures • Physiology • Knowledge Laboratory Management • Laboratory Experience • Aseptic Technique • Molecular Biology • Transfection • Research

Contact this candidate