Data Analysis Technical Support

GARIMELLA SUBRAHMANYESWARI

(Senior Bioinformatician)

*************@*****.*** Verona, Wisconsin 608-***-**** Senior Bioinformatician with 3+ years’ experience in Next-Generation Sequencing (NGS) data analysis (clinical and non-clinical), development and testing of bioinformatics tools. Creative thinker with hands-on exposure in client communication, project coordination within a team, and subject knowledge training. Senior Executive-Bioinformatics, Genepath Dx, Pune, India May 2023-Sep 2024

• Developed and deployed bioinformatics pipelines using BWA-GATK / bcftools-SnpEff for clinical NGS analysis

• Developed an automated tool that would extract data from NCBI database, align the biological data extracted and visualize it for inferences using shell scripting and python

• Led the clinical reporting team in clinical NGS data analysis and interpretation of variants and mutations in Oncology cases and Whole Exome Sequencing panels such as BRCA testing, aHUS etc. using standard protocols

• Analyzed primer sequences using bioinformatics approaches for development of PCR-based kits, assays, and validation with in-silico experiments

Bioinformatician, Nucleome Informatics Pvt. Ltd, Hyderabad, India July 2022-December 2022

• Analyzed the Whole Genome Sequencing data, Transcriptome data (reference based and De novo) using custom developed bioinformatics pipelines written using shell scripting

• Implemented R-scripts and Linux-based workflows for Genome-Wide Association Studies (GWAS) and QTL-analysis

• Provided one-on-one technical support for clients for custom based analysis Bioinformatics Analyst, Clevergene Biocorp Pvt. Ltd, Bangalore, India June 2021-July 2022

• Analyzed the sequenced data of 16S V3/V4, ITS2 Fungal, and Shotgun metagenome, RNA-seq (reference-based and De novo), and whole genome sequencing data using shell scripting in AWS server

• Obtained accurate results and successfully managed to meet 60-70% validation with the client’s expected outcome

• Developed bioinformatics tools in R and shell scripts for NGS-based transcriptomics, metagenomics pipeline and delivered results with data-visualization graphs/heatmaps/volcano-plots for client-oriented projects.

• Managed onboarding of clients and provided technical solutions for their queries TECHNICAL

SKILLS

• Over 3 years of expertise in developing bioinformatics pipelines using Shell scripting and Python and working in Linux integrated R, RStudio and MySQL server along with high-performance computing / cloud environment.

• Proficient in bioinformatics tools such as BWA, Bowtie – Bowtie2 and STAR aligner, GATK, bcftools and SnpEff -Snpsift for variant calling. Expertise in Omics data analysis like (WES, WGS, RNA-Seq, GWAS, QTL-Seq, Shotgun metagenomics and microarray)

• Strong analytical skills and effective client-communication skills EDUCATION

EXAMINATION UNIVERSITY INSTITUTE CGPA YEAR

M.Sc. Bioinformatics Manipal Academy of Higher

Education, India

Manipal School of

Life Sciences

7.83/10.0 July’20

B.Sc. Biotechnology Mumbai University, India The Wilson College 9.42/10.0 Jun’18 Journal PUBLICATIONS

• Alotaibi, A., Gadekar, V. P., Gundla, P. S., Mandarthi, S., Ravi, S., Mallya, D., Tungekar, A., Lavanya, B. V., Bhagavath, A. K., Cordero, M. W., Pitkaniemi, J., Seetharam, R. N., Bepari, A., & Hebbar, P. (2023). A comprehensive analysis of mRNA expression profiles of Esophageal Squamous Cell Carcinoma reveals downregulation of Desmoglein 1 and crucial genomic targets. Cancer biomarkers: section A of Disease markers, 38(4), 465–487. https://doi.org/10.3233/CBM- 230145

• Subrahmanyeswari Ravi, Joel Rodrigues, et al; Assessment of microbial load and drug resistant staphylococcus aureus contamination on train handles of western railways in Mumbai, International Journal of Enhanced Research in Science, Technology & Engineering, ISSN: 2319-7463, Vol. 11, Issue 3, March-2022. National Centre for Biological Sciences, TIFR (Master’s thesis project) Jan 2020-July 2020

• Bioinformatics- Performed small RNA sequencing analysis with an aim to identify the differentially expressed 24-nt sRNA, check their effect on epigenetic marks and gene expression and further to study the role of small RNAs in RNA- Directed DNA Methylation in Plants

PROFESSIONAL EXPERIENCE

ACADEMIC PROJECTS

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