R D Solution Consultant

Yiyun Wang, Ph.D.

Phone: 650-***-**** Email: *****.*****@*****.***

Highlights

Highly innovative and motivated scientist with over 20 years of experience in molecular and cellular biology. Expert in gene editing, mammalian and bacterial cell culture, immune cell identification and isolation, and high-throughput screening using ELISA and FACS. Highly adaptable with a strong willingness to learn and master new skills as needed.

Successfully led about 60 gene editing projects, including donor DNA design, gRNA screening, RNP transfection in 293T cells, RNP electroporation in iPSCs, clone screening and banking, PCR, sequencing, and data analysis. Developed ELISA kits, AAV potency assays, adenovirus and enterovirus detection assays, and NK cell killing assays. Established an NK cell differentiation system and engineered CAR-T cells via lentiviral vectors and CAR-NK cells via retroviral vectors.

Authored standardized protocols for iPSC gene editing, GMP iPSC culture, and quality control. A results-driven leader with strong project management and troubleshooting skills. Professional Experience

IASO Biotherapeutics Senior Scientist Jan. 2022 – Present

• Developed reporter cell lines and luminescence assays,

• Engineered CAR-T cells using lentiviral vectors,

• Engineered CAR-NK cells using retroviruses vectors,

• Screening CAR binders with NK killing and T killing assays,

• Developed 3D differentiation of NK cells from iPSC system under defined, feeder-free conditions

• Designed gene editing projects, identified new CAR loci, and optimized promoters for stable CAR expression,

• Developed a CRISPR/Cas9-based iPSC CAR knock-in system to generate CAR-iPSC cell lines,

• Generate iPSC-derived CAR iNK cells and evaluated their efficacy,

• Trained scientists in iPSC culture and gene editing. Applied Stemcell Inc. Scientist Nov. 2020-Jan. 2022

• Developed AAV potency assay to evaluate gene therapy product efficacy,

• Generated iPSC-derived photoreceptor cells and used them to test AAV transfection MOI,

• Established an NK cell differentiation system and developed an NK killing assay,

• Developed CRISPR gene knock-in system without a selection marker,

• Authored iPSC gene editing protocol, GMP iPSC culture protocols, bench sheets, QC protocols,

• Set up MSC culture medium production system,

• Developed mammalian cell culture and banking SOPs for clients,

• Provided technical support to the marketing team for new projects, communicated and prepared customer reports.

Cel Analytical Inc. Scientist Sep. 2019-Mar. 2020

• Developed a standardized protocol for detecting adenoviruses and enteroviruses in environmental samples.

DiaCarta Inc. Scientist Mar. 2019-Sep. 2019

• Conducted manufacturing, QC, V&V testing for molecular diagnostics and IVD kits using qPCR. Tribioscience Inc. Scientist Apr. 2018-Mar. 2019

• Developed ELISA kits and stem cell culture media,

• Authored study protocols and reports for sponsor clients and FDA review. Applied Stemcell Inc. Consultant Nov. 2012-Apr. 2013

• Stem cell culture and generation of iPSCs from human somatic cells,

• Then contract ended due to visa-related limitations. Johns Hopkins University School of Medicine Postdoc Sep. 2008-Apr. 2011

• Developed a genetic inducible lineage tracing system in zebrafish,

• Established a Cre-Lox lineage tracing system,

• Generated multiple zebrafish lines for research,

• Identified Notch-responsive cells as β-cell progenitors and precursors of other endocrine cells,

• Developed a novel immunofluorescence protocol, an RNA FISH assay and a PCR assay,

• Supervised and mentored research assistants,

• Organized the 2nd Annual Postdoc Poster Symposium of The Johns Hopkins School of Medicine. Peking University, China Postdoc 2006-2008

• Investigated the role of Girdin protein in human DNA replication initiation. Cloned the girdin gene fragment and produced anti-Girdin antibody. Performed Western blotting and immunofluorescence to study Girdin localization within cells. Used siRNA to suppress endogenous Girdin expression in 293A cells to examine its role in the cell cycle.

• In charge of writing grant applications and successfully secured a grant from the National Natural Science Foundation of China in 2007 for the discovery of a novel DNA replication initiation protein, Sap1, and its functional studies in fission yeast (Grant No. 30770441/C01040101). Dalian University of Technology, Dalian, China Ph.D.

• Establishment a system for foreign gene expression in Chlorella sp.

• Supervised and mentored graduate and undergraduate students. Fushun Petroleum Institute, Fushun, China B.S.

Publications

1. Yiyun Wang, Meritxell Rovira, Shamila Yusuff, and Michael J. Parsons. Genetic inducible fate mapping in larval zebrafish reveals origins of adult insulin producing β-cells. Development. 2011, 138(4):609-17. 2. Yiyun Wang, Zheng Xulong, Wang Changhai. The Expression of Phytase Gene in Chlorella Sp. MACC/C95. Journal of Harbin Institute of Technology. 2008, 8: 112-116. 3. Yiyun Wang, Gao Xiaorong, Wang Changhai. Construction of a System for the Stable Expression of Foreign Genes in Chlorella sp. High Technol Lett. 2007, 13(1): 91-94. 4. Changhai Wang, Yiyun Wang, and Xiaorong Gao. Transient Expression of the GUS Gene in a Unicellular Marine Green Alga, Chlorella sp. MACC/C95, via Electroporation. Biotechnol. Bioprocess Eng. 2007 12, 2: 180-183.

5. Yiyun Wang, Wang Changhai. Study on Selection marker of genetic engineering in Chlorella sp. Journal of Dalian University of Technology. 2007, 47(4): 509-514. 6. Chang-Hai Wang, Yiyun Wang, Ying-Ying Sun, Xian-Tang Xie. Effect of antibiotic treatment on toxin production by Alexandrium tamarense. Biomedical and Environmental Sciences, 2003, 16: 340-347. 7. Wang Changhai, Yiyun Wang, Tian Zhili. High Density Culture of Porphyridium cruentum in a 42 L Internal Airlift Loop Photobioreactor, The Chinese Journal of Process Engineering, 2003, 3(6): 565-569. Talks

1. Yiyun Wang, Meritxell Rovira, Shamila Yusuff, and Michael J. Parsons. Genetic inducible fate mapping in larval zebrafish reveals origins of adult insulin producing β-cells, Dec.16, 2010. Mid-Atlantic Regional Zebrafish Meeting

2. Yiyun Wang, Josh D.Eikenberg, Michael J. Parsons. Using cre-lox to lineage trace pancreatic progenitors. Nov. 20, 2009. Baltimore Area Zebrafish Meeting

Abstracts and Posters

1. Identification of Pancreatic Progenitors in the Zebrafish Pancreas. Yiyun Wang, Josh D. Eikenberg, Michael J. Parsons. Jun. 16-20, 2010. 9th International Meeting on zebrafish development and genetics. 2. Identifying Chemical Inducers of Beta-cell Neogenesis. Shamila Yusuff, Yiyun Wang, Joong Sup Shim, Jun Liu, Michael J. Parsons. Jun. 16-20, 2010. 9th International Meeting on zebrafish development and genetics.

3.Using cre-lox technology to identify pancreatic cell progenitors in larval zebrafish, Yiyun Wang, Josh D. Eikenberg, Michael J. Parsons. Jun. 4-5, 2010. SDB Mid-Atlantic Regional meeting. 4. Using cre/lox to lineage trace notch responsive cells in the larval zebrafish pancreas. Yiyun Wang, Josh D. Eikenberg and Michael J. Parsons, Feb,26th,2010. JHPDA 2nd Annual postdoc Poster symposium 5. Identification of β-cell progenitors in the larval zebrafish pancreas. Yiyun Wang and Michael J. Parsons, May 15-16, 2009. SDB Mid-Atlantic Regional meeting

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