Assistant Professor
Resume:
Dr. Uzma Zaman
PhD, Postdoc (Germany)
****.*****@****.***.**
Education
• 2015: Postdoctoral research fellow at Bioanalytical Mass Spectrometry Group, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany.
• 2009: PhD (Protein Chemistry)
International Center for Chemical and Biological Sciences, HEJ research institute of Chemistry, University of Karachi.
• 1998: M.Sc (Biochemistry)
Department of Biochemistry, University of Karachi
Work Experience
• 2018–current: Assistant Professor at Department of Biochemistry, Dow University of Health Sciences, Ojha campus Karachi.
• 2016–2018: Assistant Professor at Dow Research Institute of Biotechnology and Biomedical Sciences, Dow University of Health Sciences, Ojha campus Karachi.
• 2015–2016: Project manager at, ICCBS, H.E.J. Research Institute of Chemistry, University of Karachi, Pakistan.
• 2010–2015: Postdoctoral research fellow at Bioanalytical Mass Spectrometry Group, MPI for Biophysical Chemistry, Gottingen, Germany.
• 2009–2010: Assistant Professor at National Center for Proteomics, University of Karachi, Pakistan.
• 2009: Senior research fellow at Protein Chemistry group, ICCBS, H.E.J. Research Institute of Chemistry, University of Karachi, Pakistan.
• 2008: Volunteer in Mass Spectrometry Section at, ICCBS, H.E.J. Research Institute of Chemistry, University of Karachi, Pakistan.
• 2006-2007: Visiting fellow at Redox Biology Center, university of Nebraska, Lincoln, NE, USA.
2
• 1999-2008: PhD student at ICCBS, H.E.J. Research Institute of Chemistry, University of Karachi, Pakistan.
Publications
• 2024: Zaman U, Abbasi A, MALHI SM. Identification and characterization of serine proteases from cuminum cyminum (apiaceae). Pak. J. Bot. 2024 Feb 1;56(1):127-33. http://dx.doi.org/10.30848/PJB2024-1(2)
• 2023: Azmi MB, Ahmed A, Ahmed TF, Imtiaz F, Asif U, Zaman U, Khan KA, Sherwani AK. Transcript-Level In Silico Analysis of Alzheimer’s Disease-Related Gene Biomarkers and Their Evaluation with Bioactive Flavonoids to Explore Therapeutic Interactions. ACS omega. 2023 Oct 18;8(43):40695-712. https://doi.org/10.1021/acsomega.3c05769
• 2023: Ahmed TF, Imtiaz F, Zaman U, Ahmed A, Shahbaz N. Comparison of potential plasma biomarkers in Alzheimer’s disease and neurodegenerative dementias in Pakistani population. JPMA. The Journal of the Pakistan Medical Association. 2023 Oct 1;73(10):2062-4. https://doi.org/10.47391/JPMA.7286
• 2023: Majeed HK, Majeed S, Leghari QA, Malhi SM, Shuja AA, Rizvi SA, Zaman U, Kashif SS. Mangiferin Produces Neuroprotective Effect By Attenuating H2o2 Induced Oxidative Stress In Primary Rat Hippocampal Neural Progenitor Cells. Journal of Survey in Fisheries Sciences. 2023 Jul 6:682-7.
• 2023: Ahmed TF, Azmi MB, Imtiaz F, Zaman U, Ahmed A, Shahbaz N. Plasma levels of phosphorylated tau and neurofilament light chain as potential biomarkers for Alzheimer’s disease: A biochemical analysis in Pakistani population. Saudi Pharmaceutical Journal. 2023 Jul 1;31(7):1202-9.
• 2022: Saleem Z, Shaikh AH, Zaman U, Ahmed S, Siddiqui Z, Majeed MM, Kazmi A. Analytical study of salivary MMP-12 expression in oral submucous fibrosis. Journal of Ayub Medical College, Abbottabad: JAMC. 2022 Apr 1;34(2):247-50.
• 2021: Javed L, Hanif F, Malhi SM, Zaman U, Jahan N, Amir Q, Javed A, Malik AB, Abrar H. Diclofenac sodium enhances the antiepileptic effect of levetiracetam in pilocarpine induced epileptic mice model. Pakistan Journal of Pharmaceutical Sciences. 2021 Sep 2;34.
3
• 2021: Saleem Z, Shaikh AH, Zaman U, Ahmed S, Majeed MM, Kazmi A, Farooqui WA. Estimation of salivary matrix metalloproteinases-12 (MMP-12) levels among patients presenting with oral submucous fibrosis and oral squamous cell carcinoma. BMC Oral Health. 2021 Apr 23;21(1):205. doi.org/10.1186/s12903-021-01571-7.
• 2018: Baig S, Zaman U, Lucky MH. Human papilloma virus 16/18: Fabricator of trouble in oral squamous cell carcinoma. International Journal of Infectious Diseases. 2018 Apr 1;69:115-9. doi:10.1016/j.ijid.2018.02.003.
• 2018: Zaman U, Urlaub H, Abbasi A. Protein Profiling of Non model Plant Cuminum cyminum by Gel Based Proteomic Approach. Phytochemical analysis. 2018 May;29(3):242-9.DOI 10.1002/pca.2738.
• 2015: Zaman U, Richter F, Hofele R, Kramer K, Sachsenberg T, Kohlbacher O, Lenz C, Urlaub H. Dithiothreitol (DTT) Acts as a Specific, UV-inducible Cross-linker in Elucidation of Protein–RNA Interactions*[S]. Molecular & Cellular Proteomics. 2015 Dec 1;14(12):3196-210. DOI:https://doi.org/10.1074/mcp.M115.052795
• 2009: Zaman U, Abbasi A. Isolation, purification and characterization of a nonspecific lipid transfer protein from Cuminum cyminum. Phytochemistry. 2009 May 1;70(8):979-87.
• 2008: Gherasim CG, Zaman U, Raza A, Banerjee R. Impeded electron transfer from a pathogenic FMN domain mutant of methionine synthase reductase and its responsiveness to flavin supplementation. Biochemistry. 2008 Nov 25;47(47):12515-22. Area of interest
• Purification of immunomodulatory proteins from medicinal plants
• In vivo and in vitro strategies to identify protein-protein and protein-RNA interaction by mass spectrometry.
• Purification of Rabies vaccine in vero cell line
• Proteome profiling of plant proteins and venom proteins.
• Identification of protein biomarkers in Saliva from squamous cell carcinoma patient. 4
Technical Skills
• Protein Purification by Gel filtration, Ion Exchange, Affinity and Reverse phase chromatography.
• Synthesis, purification and labeling of oligonucleotides.
• Protein characterization by 1D and 2D Electrophoresis.
• Protein identification based on Peptide Mass Fingerprint, LC-MS/MS
• Identification and quantification of proteins for mass spectrometry-based proteomics
• Probing protein conformational changes and folding behavior by Hydrogen/Deuterium exchange mass spectrometry.
• Detection and analysis of Protein-protein and Protein-RNA interaction by photo/chemical cross linking by mass spectrometry.
• Experience with Mass Spectrometer Q-Exactive HF, LTQ Orbitrap Velos and LTQ orbitrap XL from Thermo Scientific, ESI-MS/MS QSATR XL, QTrap 4000 from ABSCIEX, QToF-Ultima from Water, Ultraflex MALDI ToF/ToF from Bruker and. 4800 MALDI ToF/ToF from ABSCIEX.
• Denovo sequencing of protein after CID, HCD Fragmentation.
• Experience of working in Radioisotope Laboratory and handling of Radioactive materials.
• UV/Visible and Circular Dichroism Spectroscopy.
Teaching Experience
• Teaching Biochemistry to undergraduate and postgraduate student at different collages of Dow University of health Sciences
• Hands on training of HPLC to research students at Institute of Basic Medical Sciences
• Teaching Assistant at Bioanalytical Mass Spectrometry Group MPI for Biophysical Chemistry, Gottingen, Germany. Conducted Lecture and labs for MS and PhD student on basic and advanced mass spectrometry techniques.
• Supervised Mass Spectrometric research projects at Bioanalytical Mass Spectrometry Group MPI for Biophysical Chemistry, Gottingen, Germany. 5
1. UV-induced protein-RNA crosslinking in E. coli
2. Mapping of protein-RNA interaction sites in U1-snRNP
• Instructor of protein mass spectrometric techniques at ICCBS, H.E.J. Research Institute of Chemistry, University of Karachi, Pakistan Presentations
Evaluate the Anti-Inflammatory Potential of Biological active proteins from Cuminum cyminum, in 1
st
International Conference on biomedical Sciences, Dow University of Health Sciences, Karachi, Pakistan, on March 20 th
-21
th
, 202.
Purification and Characterization of Anti-inflammatory Protein from Solanum Nigrum, in Cloister of San Pietro in Vincoli, venue of the Faculty of Civil and Industrial Engeener of Sapienza University, Rome, Italy, on Sep 5-8
th
, 2018.
Use of Photoreactive Base Analogues to Study Protein-RNA interaction by Mass Spectrometry, in 14th Eurasia on Chemical Sciences, at ICCBS, University of Karachi, Pakistan on Dec 15
th
- 18
th
, 2016.
Analyses of pre-mRNA splicing machinery by UV-induced protein-RNA cross-linking and mass spectrometry, in International Conference on Translational medicine: from discovery to health care, at Ziauddin University, Pakistan, on Feb, 1-3 rd
, 2016.
DTT mediates site-specific protein/RNA crosslinking upon UV irradiation, in 62 nd
ASMS Conference on Mass Spectrometry and Allied Topics at Baltimore Convention Center, Maryland, USA., on Jun 15
th
- 20
th
, 2014.
Analyses of yeast mRNPs by the use of the photo-reactive nucleobase 4-thio- uracil and mass spectrometry in 61
st
ASMS Conference on Mass Spectrometry and Allied
Topics at Minneapolis Convention Center, Minneapolis, MN, USA., on Jun 9 th
- 13
th
,
2013.
Application of OpenMS pipline for the investigation of protein-RNA crosslinking by mass spectrometry in 5
th
OpenMS User Meeting on High-performance software for high-throughput proteomics and metabolomics at University of Salzburg, Austria, on Oct 8
th
-9
th
, 2012.
6
Analyses of Protein–Nucleic Acid complexes by MS – Reactivity of Various Amino Acids with Nucleotides upon UV Irradiation in 60
th
ASMS Conference on Mass
Spectrometry at Vancouver, BC, Canada, on May 20
th
-24
th
, 2012.
Mass-spectrometric investigation of 6-thioG-substituted RNA-oligonucleotides cross- linking to proteins in 8
th
international symposium, Horizons in molecular biology at Max Planck Institute for Biophysical Chemistry, Gottingen, Germany, on Sep 14 th
-17
th
,
2011.
Mapping of conformational changes in MeaB after ligand binding by Hydrogen/Deuterium Exchange Mass spectrometry” in 9 th
international symposium on
Protein structure and function relationship at H.E.J research Institute of chemistry, University of Karachi, on Jan 9
th
-11
th
, 2007.
Activity directed isolation of serine proteinase from cuminum cyminum in 7 th
biennial
conference on Trends in biochemistry and molecular biology at institute of biochemistry and biotechnology, University of Punjab, Lahore, on Apr 2 nd
-5
th
, 2003.
Isolation and characterization of a serine proteinase from cuminum cyminum in 7 th
international symposium on Protein structure and function relationship at H.E.J research Institute of chemistry, University of Karachi, on Jan 20 th
-24
th
, 2003.
Conference and workshops organizer
Workshop on "Research Tools in Proteomics", in National Center for Proteomics, University of Karachi, on Nov 16
th
-18
th
, 2009.
9
th
international symposium on Protein structure and function relationship at H.E.J research Institute of chemistry, University of Karachi, on Jan 11 th
-14
th
, 2007.
8
th
international workshop on Characterization of proteins at H.E.J research Institute of chemistry,, University of Karachi, on Jan 11
th
-18
th
, 2005.
8
th
international symposium on Protein structure and function relationship at H.E.J research Institute of chemistry,, University of Karachi, on Jan 7 th
-10
th
2005.
Workshop on Characterization of proteins at H.E.J research Institute of chemistry,, University of Karachi, on Jan 27
th
-Feb 5, 2003.
7
7
th
international symposium on Protein structure and function relationship at H.E.J research Institute of chemistry, Jan 20
th
-24th 2003.
Technical trainings and courses
Q-Exactive HF operations, training course, provided by thermo scientific, at Max Planck Institute for Biophysical Chemistry, Gottingen, Germany, on Dec 12 th
-13
th
, 2014.
Orbitrap Fusion Tribid Biotech operations, training course, provided by thermo scientific, at Max Planck Institute for Biophysical Chemistry, Gottingen, Germany on Nov 25 th
-
27
th
, 2014.
TSQ Biotech operations, course, provided by thermo scientific, at Max Planck Institute for Biophysical Chemistry, Gottingen, Germany on June 21th to 23rd, 2011.
Orbitrap Velose operations, training course, provided by thermo scientific, at Max Planck Institute for Biophysical Chemistry, Gottingen, Germany, on Jun 19 th
-20
th
,
2011.
Radiation safety training at Max Planck Institute for Biophysical Chemistry, Gottingen, Germany, on Jan 14
th
-17
th
, 2011.
Lecture series on survey of mass spectrometry at International center for chemical and biological sciences, university of Karachi, on Mar 2 nd
-26
th
, 2009.
Certificate course and training of HPLC and Unipoint System Software by GILSON at H.E.J research Institute of chemistry, on jul 26
th
-30
th
, 2001.
Certificate course of X-ray crystallography at H.E.J research Institute of chemistry, on May 2000.
Computer Skills
• Proteomic Analysis softwares: Proteome Discoverer (Thermo Scientific), Scaffold
(Proteome Software), Mascot search algorithm (Matrix Sciences), MaxQuant, OpenMS tool RNP
XL
• Microsoft Office: Word, Excel, Access, Power Point 8
Memberships in scientific organization
American society for mass spectrometry
American chemical society
German Society of Proteome Research
Eurasia Science Organization
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