Eeshika Mitra, BDS, MPH

adydye@r.postjobfree.com

https://www.linkedin.com/in/eeshikamitra/

Cell: +1-979-***-****

SUMMARY

I am a Life Sciences Research Professional with a degree in dental sciences working in Byer’s Eye Institute in Stanford, Ophthalmology with experience in the fields of Clinical Research trials, Regulatory Compliance, and basic sciences. I have worked at several labs and have extensive research experience in Life Sciences (Molecular Biology, Cell Biology, and aging related disorders)

EDUCATION

Texas A&M University, School of Public Health

College Station, TX, December,2016

Master of Public Health, Epidemiology

Mahatma Gandhi Post Graduate Institute of Dental Sciences Puducherry, India, October,2013 Bachelor of Dental Surgery

Regulatory Affairs and Compliance (1 year program) – Certified by University of California at Irvine- In Progress SKILLS

Technical Skills:

• Proficient in use of confocal microscopy and inverted scopes, immunohistochemistry, cell culture work

• Mice retinal dissections, cryosectioning, perfusion, mice/rat handling work (includes conducting behavioral experiments, mouse colony maintenance), genotyping, running qPCR.

• Performing western blots and ELISA tests and basic molecular techniques, plasmid construction for AAV (viral packaging), bacterial plasmid manipulation, maxi and mini preps for bacterial cell culture work

• Use of BD Fortessa Flow Cytometer, OCT (Optical coherence tomography for mice retina), BioRad thermal cycler and ultra centrifuge

• Use of Heidelberg software for analysis, Fiji Image Analysis software, Imaris- Cell imaging software, Python (deep learning methods) for automated cell quantification, simple neurite tracer use of snapgene, proficient in EPIC Software use, SAS data analysis software, STATA, Bio-Rad Data analysis for analyzing qPCR data and application of graph pad prism software

Writing: Scientific and Technical Writing, Endnote [Citation Management], Good Documentation Practices IACUC -Protocol Submissions, Amendments, and training updates for lab personnel for various NIH projects PUBLICATION

• Manuscript -1, Journal of aging and disease

Upadhya, D., Kodali, M., Gitai, D., Castro, O. W., Zanirati, G., Upadhya, R., ... & Shetty, A. K. (2019). A model of chronic temporal lobe epilepsy presenting constantly rhythmic and robust spontaneous seizures, co-morbidities, and hippocampal neuropathology. Aging and disease, 10(5), 915.

• Manuscript -2, Journal of Controlled Release

Corresponding Author: Professor Ashok K Shetty

Co-Authors: Sahithi Attaluri, M Pharm; Meenakshi Arora, PhD; Leelawati N. Madhu, PhD; Maheedhar Kodali, PhD; Bing Shuai, M.D.; Laila Melissari, M.S.; Raghavendra Upadhya, PhD; Xiaolan Rao, B.S.; Adrian Bates, B.S.; Eeshika Mitra, M.P.H.; Keyhan R. Ghahfarouki; M N. V. Ravikumar, PhD

• Peer reviewed Manuscript in Neuroscience Insights (Sage Journals) entitled “Role of Citocoline in patients with mild cognitive impairment “

• Manuscript in preparation 1- The manuscript hypothesizes that candidate neuroprotective therapies will not have correlated effects between neuroprotection and RGC electrophysiology and circuit preservation, and that different candidate therapies will affect different RGC subtypes.

• Manuscript in preparation 2- The working title of the manuscript is “Re- expression of developmental transcription factors induces retinal ganglion cells that project to visual targets”. The manuscript hypothesizes harnessing the developmental potential of Müller glial cells to enable retinal ganglion cell generation. RESEARCH AND WORK EXPERIENCE

Stanford School of Medicine – Department of Ophthalmology, Byer’s Eye Institute August 16, 2021, to Present

• Molecular biology techniques that include (isolation, purification and maintenance of bacteria, bacterial transformation), plasmid construction (using Snapgene) for AAV packaging and thereby preparing for subretinal injections in mice for retinal gene transfer into developing retinas for in vivo analysis of retinal genes

• Mice eyeball retinal dissections that were followed by immunohistochemistry especially RBPMS staining (for retinal ganglion cells) and confocal imaging for quantification of surviving retinal ganglion cells, optic nerve staining (PPD stain) to quantify surviving axons and axons regeneration.

• Silicon oil injections (anterior chamber of the eye) for mice model of Glaucoma and IOP (intraoperative pressure measurements), intravitreal injections of AAV in the eye

• Assist with rodent eye surgeries and OCT (optical coherence tomography in mice), in situ imaging of fundus of the eye and the retina.

Research Associate II- Immunology, Lung Institute, Cedar Sinai Medical Center, Beverly Hills, CA February 2020-Present

• Assisting in several molecular biology techniques such as Western Blots, ELISAs, PCR- for genotyping (MNX mice), q- PCR to determine Cre heterozygosity.

• Flow cytometry experiments and cell culture work pertaining to growth and maintenance of Bone marrow macrophages and dendritic cells while also assisting in specific study protocols pertaining to mice handling of malignant tumor models.

Health Information Analyst- West Anaheim Hospital Medical Center -Prime Health Care Services, Anaheim, CA January 2019-2020

• Responsible for planning, organizing, and conducting departmental activities and ensuring security and maintenance of the legal medical record (involved patient interaction) and tracking Release of information workflows in Epic.

• Developed a weekly update dashboard to track the compliance of data for reporting to the Privacy officer for smooth functioning of the revenue cycle and presenting this data to the hospital leadership on behalf of the HIM department.

• Providing analytical support and quantifying business requirements and collaborating with authorizations, admitting teams, coding team and assist physicians to complete deficiencies and stay in compliance with Joint commission requirements.

Clinical Research Coordinator - Discovery Medical Management services Rocky Creek, Missouri city, Houston August - December, 2018

• Prescreening and enrolling medically compromised patients in phase 3 and phase 4 randomized clinical trials for diabetes medication, maintaining privacy and confidentiality of patient health information, maintaining source documentations, preparing charts for monitoring visits, ensuring proper completion of Case report forms (CRFs) and performing data entry into electronic data capture systems such as I-Medidata and EHRs such as e- clinical works and Epic, recruitment of patients for based on eligibility criteria

• Performing clinical duties that include recording patient’s medical history and vital records, phlebotomy and performing ECGs, processing, and shipping ambient and frozen lab samples. Research Assistant- Institute of Regenerative Medicine, Texas A&M Health Science Center, College Station, TX- January 2017- May 2018

• Research skills related to handling human induced pluripotent stem cells (HiPSC) in BSL-2, tissue culture of cholinergic neurons and rat tissue processing.

• Immunostaining (IBA1, NPY, PV DCX), use of stereo investigators, confocal imaging (exosomes, inflammatory markers, HNA, chat positive neurons in hippocampus and several other brain regions and the use of the NIS Elements software for analysis) and animal work that involved induction of status epilepticus in mice and rats, intranasal administration of exosomes .The independent project includes studying the distribution of cholinergic neurons in the medial septum, striatum, and hypothalamus regions in gulf war illness model rats. Research Assistant- The University of Texas at Austin, Waggoner Center for Alcohol and Addiction Research, College of Natural Sciences, Department of Neuroscience May- July 2018

• Histology of Rodent Brain Tissue - perform routine histology of rodent brain tissue, including cryosectioning of brain, mounting, bright field or confocal analysis of tissue and immunohistochemistry. Epidemiology Intern- Waco McLennan County Public Health District-Waco, TX, June August,2016

• Analyzing a de-identified dataset of gastrointestinal illnesses in Waco McLennan County using Epi Info Software, SAS, and Excel to clean the data and performing descriptive analysis to find out the distribution of the conditions such as Salmonellosis, Campylobacteriosis, Shigellosis, Cyclosporiasis and creating incidence maps using Arc GIS Software

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