David R. Martinez

adyag7@r.postjobfree.com

**** ***** **.

Houston, TX 77074

713-***-**** (home)

WORK SKILLS

performed lab tests per SOP and recorded results in a GMP/GLP environment

performed routine calibration of lab equipment, including spectrometers; lab balances, etc

proficient in Microsoft Office, including Word and Excel

skilled in preparing and modifying SOPs

skilled in maintaining a lab database, and the use of LIMS and Watson

trained in use of HPLC, GC, spectrophotometers (both IR and µv-visible) and pH meter

experienced in the use of robotic sampling devices (TomTec)

received training as an Internal Auditor and participated in company audits

experienced in use of GeneChips (microarrays)

extracted and purified RNA and genomic DNA

experienced in cell culture and sterile technique, including sterile gowning

experienced in working in a clean room in a semiconductor facility

experienced with preparing and analyzing Enzyme Immunoassays (ELISA)

genotyped patient samples using DNA sequencing, PCR and gel electrophoresis

proficient with RIA, and Southern and Northern blots

BIOTECH/ACADEMIC

EXPERIENCE

Ramco Laboratories, Inc. Quality Control Manager

12/13 to 1/20 Stafford, TX

received certification as an Internal Auditor (ISO 13485:2003)

participated in company audits for ISO 13485 and FDA audits

Prepared reagents used in production of medical diagnostic test kits (ELISA and Cand-Tec)

performed QC tests on the kits to ensure that they met specifications prior to sale

ensured that records in the Quality Control laboratory were filled out and kept up to date

PRACS Institute, FKA Cetero Research Extraction Chemist

6/10 to 10/12 Houston, TX

Pipetted aliquots of client study samples for wet-chemical extractions, including liquid-liquid, solid phase and direct precipitation, to prepare them for analysis by LC/MS

conducted data analysis of study samples for drugs in biological fluids, as per documented method and SOPs in a GLP environment, using Analyst, Watson, LIMS, and Microsoft Office

prepared and performed ELISAs

Kelly Scientific Associate Scientist II

9/09 to 12/09 Houston, TX

Temporary position:

Extracted and purified DNA and RNA samples

performed Southern and Northern blots as per documented method and SOP in a GLP/GMP environment

performed phage testing and plasmid loss utilizing cell culture techniques

Seqwright, Inc. Research Associate III

7/09 to 9/09 Houston, TX

Prepared DNA shotgun libraries for 454 Next Generation sequencing

Cogenics, a Beckman Coulter Genomics company Associate Scientist II

2/07 to 7/09 Houston, TX

Measured DNA and RNA concentrations using Nanodrop apparatus

Analyzed for possible RNA degradation using the Agilent 2100 Bioanalyzer

extracted and purified DNA samples

performed Southern and Northern blots

performed phage testing and plasmid loss utilizing cell culture techniques

performed restriction enzyme digests

performed QC using agarose gels participated in development of protocols

performed instrument validations and maintenance

experienced in SOP’s, GLP/GMP and safety guidelines

University of Texas Medical Branch Research Associate II

1/05 to 2/07 Galveston, TX

employed microarray technology (GeneChips) to analyze RNA samples

measured RNA concentrations using NanoDrop apparatus

extracted and purified RNA samples

analyzed for possible RNA degradation using the Agilent 2100 Bioanalyzer

performed bacterial expressions

did isolations

OTHER US Census, US Department of Commerce Census enumerator

Houston, TX

04/10 to 06/10 Participated in temporary position interviewing US residents for US Census 2010.

Harris County Election worker

Houston, TX

10/13 to 12/13 Employed in temporary position entering data for Harris County Elections and producing election forms

other positions:

Houston, TX

In the molecular biology labs

performed quantitative determinations of GHb levels in humans, employing a colorimetric method, column chromatography, and a µV-visible spectrophotometer

measured linoleic acid levels using a HPLC; genotyped diabetes type II patients, by extracting DNA and performing PCR and automatic sequencing

other duties included running RIA's, running gels, doing restriction enzyme digests, and performing Southern transfers, minipreps, subcloning, general solution preparation, and hybridizations

In the chemical/environmental lab

analyzed various materials, utilizing an optical emission spectrograph

Material tests included scales, rust, paint, metals of varying composition, polypropylene, polyethylene, etc.

Other responsibilities included sampling clients' wastewater, testing the strength of concrete, and performing wet-chemical tests on soil, water, and wastewater samples, according to ASTM and EPA methods

In the environmental research lab

analyzed water and wastewater samples, using wet-chemical techniques, for the presence of nitrates, nitrites, phenol, cyanide, phosphate, TKN, chloride, etc.

utilized a pH meter, TOD analyzer, TOC analyzer, and a µV-visible spectrophotometer.

Other responsibilities included participation in sampling trips to client sites

ACTIVITIES Member of American Chemical Society.

PUBLICATIONS "Computer Calculations in the Quantitative Analysis Course," Parker GA, Martinez DR, et al., Journal of Chemical Education, vol. 56, May, 1979, p. 309.

"Molecular Basis of Phenylketonuria and a Correlation between Genotype and Phenotype in a Heterogeneous Southeastern US Population," Eisensmith RC, Martinez DR, Kuzmin AI, et al., Pediatrics, vol.97 (4), April, 1996, pp. 512-6.

“Molecular correlations in phenylketonuria: mutation patterns and corresponding biochemical and clinical phenotypes in a heterogeneous California population,” Enns GM, Martinez DR, Kuzmin AI, et al., Pediatric Research, vol 46, pp. 594-602.

HONORS Member of Marquis’ “Who’s Who in Science and Engineering,” "Who's Who in America," and “Who’s Who in the World”.

CERTIFICATION ISO 13485:2003 Internal Auditor (TPECS).

EDUCATION University of Toledo

Bachelor of Science, Chemistry

Contact this candidate