Customer Service Research Assistant

Janette M. Hakimi, Ph.D.

**** ****** ***** **., ***. 415

Plano, TX 75023

Phone: 469-***-****

E-mail: *************@*****.***

Citizenship: U.S.

Professional Profile

Highly accomplished Molecular Biologist with both industry and academic experience. Proven ability to

consistently repeat experimental procedures and manage large data sets. Adept communicator with strong

analytical, computer, and problem-solving skills. Highly motivated, organized, accurate, and ethical.

Specialized Knowledge, Skills & Experience:

●Excellent market research capabilities. Demonstrated ability to work as part of a business team to help with the design and marketing of products for MicroArray applications.

●Expert knowledge of biochemistry and molecular biology theories, principles, and techniques gained through rigorous academic training and research.

●Excellent technical capabilities. Demonstrated ability to work independently or as part of a team to determine experimental approach; choose and perform analyses and devise protocols; and apply or modify scientific procedures and methods to solve problems.

●Published author and accomplished presenter. Fourteen published articles; including six first author publications, eight abstracts, and a book chapter.

Work Experience

Technical Associate April 2000 to July 2002

Invitrogen/Life Technologies Inc. (www.invitrogen.com)

1600 Faraday Ave., Carlsbad, CA 92008

Contact: Kevin Grady 240-***-****

Recruited to provide technical service and support for Invitrogen/LTI molecular biology products. Invitrogen is the leading supplier of cell culture products, service and technologies for research and biopharmaceutical manufacturing applications.

Provided telephone-based technical assistance to over 45 customers daily for product-related problems, including optimizing PCR protocols for sensitive and specific amplification of the sequences of interest.

Part of a team responsible for generating a portfolio of the company’s MicroArray products.

Interfaced with product managers, and manufacturing teams to resolve customer complaints and issues. Assisted Tech-Online team with internet-based customer service.

Accomplishments:

As a result of extensive and broad scientific knowledge, as well as capability to establish a rapport with customers, became the Department point person for product information on MicroArrays. Played key role in the successful marketing of products for this application, which enabled Invitrogen to create a new business unit.

Consistently received exemplary performance ratings for contributions in the areas of customer service, product marketing, and database management.

Postdoctoral Fellowships

Oncology Postdoctoral Fellow April 1998 to April 2000

Johns Hopkins University/Johns Hopkins Hospital

School of Medicine, Department of Oncology

Bunting Blaustein Bldg., Rm. 162

1650 Orleans St., Baltimore, MD 21231

Contact: Ted DeWeese 410-***-****

Research Focus: Find potential prostate cancer biomarkers using the technique of differential display (DD). Experiment: Set up DD technology in the lab and looked at induction of differentiation in a number of prostate cancer cell lines using phenylbutyrate. Also set up the technique of RNase Protection Assay. Trained a lab technician and undergraduate students in the use of DD and other techniques for looking at various drug treated prostate cancer cell lines.

Results/Accomplishments:

Successfully used differential display (DD) to find a number of potential markers in prostate cancer. These targets could potentially be used for identifying drug target pathways and as markers of differentiation in prostate cancer.

Gained extensive experience in the areas of cell biology and mechanisms of transformation.

Urology Postdoctoral Fellow December 1992 to April 1998

Johns Hopkins University/Johns Hopkins Hospital

School of Medicine, Department of Urology

600 N. Wolfe St., Baltimore, MD 21287

Contact: Dr. Evelyn R Barrack 313-***-****

Research Focus: (1) Determine the frequency distribution of the androgen receptor (AR) CAG and GGC repeat length in men with prostate cancer (CaP). (2) Determine the frequency of AR gene mutations in CaP. Used PCR and SSCP (single strand conformation polymorphism) to screen genomic DNA for the coding exons of the AR gene.

Results: (1) A subpopulation of men with CaP had a substantially higher frequency of AR alleles with 16 or 17 CAGs (10%) than did the general population (1.6%) where the most common AR allele has 21 CAGs. My work suggested that short AR CAG or GGC alleles may be risk factors for the development of CaP. (2) Found a germline mutation in an organ-confined CaP sample.

Accomplishments: Presented the results from my work at several conferences (see Abstracts). Data was published in four scientific journals, a book chapter, a review and two research papers.

Research Experience

Graduate Research Assistant September 1984 to January 1992

Johns Hopkins University/Johns Hopkins Hospital

Bloomberg School of Public Health, Dept. of Biochemistry

600 N. Wolfe St., Baltimore, MD 21287

Contact: John J Scocca 410-***-****

Research Focus: Investigated the molecular biology and biochemistry of the phage HP1 site-specific recombination to determine whether the bacterial host protein IHF was involved in the process.

Result: Data demonstrated that E. coli integration host factor was involved in expression or stimulation of the HP1 recombination system (see Publications).

Accomplishment: Determined the role of integration host fact in the HP1 site-specific recombination pathway.

Developed a purification scheme to allow isolation of milligram quantities of pure integrase. Tested the effect of buffer components, IHF, and integrase protein on the rate of recombination.

Result: Data suggested the assembly of a multiprotein complex on the attP DNA substrate and a stoichiometric requirement for integrase in the reaction. Used DnaseI footprinting to identify HP1 integrase binding sites on both attB and attP segments.

Accomplishment: Two classes of binding sites were identified. Also identifed the mechanism of HP1 recombination. The integrase produces a four-stranded initial reaction product, which then undergoes a second strand transfer, to produce the final product.

Additional Research Accomplishments:

Developed a purification protocol for integrase and determined the stoichiometry of the reaction. Using DnaseI footprinting, determined the location of integrase binding sites on attB and attP segments. And also determined the order of strand exchange reaction.

Awarded Ph.D. in 1992. Thesis: “Purification and Characterization of Bacteriophage HP1 Intergrase Protein,” an investigation of the H influenzae bacteriophage HP1 integrase activity. This work resulted in three first author publications in peer-reviewed journals.

Undergraduate Research Assistant September 1982 to May 1984

College Work Study Program, University of Texas at Arlington

Dept. of Chemistry, 701 S. Nedderman Dr., Arlington, TX 76019

Contact: Kenneth L Brown 740-***-****

Research Focus: To synthesize various derivatives of Vitamin B12. In order to understand the nature of carbon-cobalt bond in Cobalamins (Vitamin B12 derivatives) carried out UV-Vis spectrophotometric titrations at several temperatures and pH values. This allowed the calculation of the pka values for the base-on-base-off transition of these derivatives.

Result: Together with 31P NMR spectra of these compounds, the data contributed to the understanding of the importance of steric effects of organic ligands, on base-on-base-off equilibria of Cobalamins.

Accomplishments: As a team, Dr. Brown and I published seven papers together. I presented my work at two meetings of the American Chemical Society in Dallas, Texas and won two undergraduate research awards.

Teaching Experience

Johns Hopkins University (1992 to 1994)

Instructor, Department of Biology

Introduction to Biological Molecules

Stanley H. Kaplan Educational Center, LTD (1992)

Instructor, Preparation for the Medical College Admission Test

University of Texas at Arlington (1983 to 1984)

Department of Chemistry, Teaching Assistant

Freshman Chemistry and Organic Chemistry Laboratories

Education

Ph.D. Biochemistry, 1992

Johns Hopkins University, Bloomberg School of Public Health

Department of Biochemistry

600 N. Wolfe Street, Baltimore, MD 21287

B.S. Biochemistry, Minor French, 1984

University of Texas at Arlington, Arlington, TX

701 S. Nedderman Dr., Arlington, TX 76019

Honors & Awards

AACR-AFLAC Scholars in Cancer Research Award (1998)

Department of Chemistry Undergraduate Research Award, (1983)

Dallas Society of Analytical Chemists Student Analyst of the Year Award (1982)

CRC Press Freshman Chemistry Achievement Award (1981)

Alpha Chi (National Honor Society)

Phi Lambda Upsilon (Honorary Chemical Society)

Technical Training & Workshops

cDNA Library Techniques, Life Technologies Training Center, Rockville, MD, 2000; Differential Display Summer Workshop, Vanderbilt University/GenHunter Corporation, Nashville, TN, (1998);

Cell Culture Techniques, Life Technologies Training Center, Rockville, MD (1997).

Computer Training

Introduction to HTML, Howard Community College, Business Training Center, Columbia, MD (2002); Siebel 2000 eBusiness, Invitrogen CRM, San Diego, CA (2001); Adobe Photoshop and Microsoft PhotoDraw, Life Technologies Training Center, Rockville, MD (2000).

Language Skills

Fluent in Persian (Dari). Familiar with French.

Conferences

American Association for Cancer Research (1994 to 1998)

89th Annual Meeting (1998), New Orleans, LA

88th Annual Meeting (1997), San Diego, CA

87th Annual Meeting (1996), Washington D.C.

85th Annual Meeting (1994), San Francisco, CA

International Symposium on Biology of Prostate Growth (1996)

Washington D.C.

Spore Investigator's Workshop (1996)

Rockville, MD

Cold Spring Harbor Symposium on Quantitative Biology, LIX (1994)

Molecular Genetics of Cancer, Cold Spring Harbor, NY

American Chemical Society (1983 to 1984)

17th Annual Meeting-In-Miniature (1984), Dallas, TX

16th Annual Meeting-In-Miniature (1983), Dallas, TX

Presentation Abstracts

1.Hakimi, J.M., and Brown, K.L. 31P-NMR Observations of Phosphodiester Protonation of the Nucleotide Loop of Methylcobalamin. Abstracts of the 16th Annual Meeting-In-Miniature, Dallas-Fort Worth Section of the American Chemical Society, Dallas, TX, April 22, 1983.

2.Schoenberg, M.P., Hakimi, J.M., Wang, S., Bova, G.S., Epstein, J.I., Fischbeck, K.H., Isaacs, W.B., Walsh, P.C. and Barrack, E.R. Microsatellite mutation (CAG24 18) in the androgen receptor gene in human prostate cancer. Proc. Amer. Assoc. Cancer Res., 85th Annual Meeting, April 10-13, San Francisco, CA, (abstract 1653) 35:277(1994).

3.Schoenberg, M.P., Hakimi, J.M., Wang, S., Bova, G.S., Epstein, J.I., Fischbeck, K.H., Isaacs, W.B., Walsh, P.C., and Barrack, E.R. Microsatellite Mutation (CAG24 18) in the Androgen Receptor Gene in Human Prostate Cancer. J. Urol. 151:458A (abstract 923)(1994).

4.Hakimi, J.M., Schoenberg, M.P., and Barrack, E.R. Androgen Receptor Variants as a Potential Risk Factor for Aggressive Prostate Cancer. J. Urol. 153:282A (abstract 216)(1995).

5.Hakimi, J.M., Schoenberg, M.P., Rondinelli, R.H., May, S.A., and Barrack, E.R. Androgen Receptor CAG (Glutamine) and GGC (Glycine) Repeat Lengths as Potential Risk Factors for Prostate Cancer.

Proc. Amer. Assoc. Cancer Res., 87th Annual Meeting, April 20-24, Washington DC, (abstract 1759) 37:258 (1996).

6.Hakimi, J.M., Schoenberg, M.P., Rondinelli, R.H., May, S.A., and Barrack, E.R. Androgen Receptor CAG (Glutamine) and GGC (Glycine) Repeat Lengths as Potential Risk Factors for Prostate Cancer. Abstracts of the 1996 International Symposium on Biology of Prostate Growth, Washington, D.C., March 28-31, (abstract 80), 1996.

7.Hakimi, J.M. Schoenberg, M.P., Rondinelli, R.H., Piantadosi, S., and Barrack, E.R. Androgen Receptor Variants with Short Glutamine or Glycine Repeats: Potential Risk Factors for Prostate

8.Cancer. Abstracts of the 4th Spore Investigators’ Workshop, July 14-16, Rockville, MD, (abstract 25), 1996.

9.Hakimi, J.M., Rondinelli, R.H., Schoenberg, M.P., Ahmed, R.L., May, S.A., Bova, G.S., Isaacs, W.B., and Barrack, E.R. Frequency of Androgen Receptor Gene Mutations in Prostate Cancer.

Proc. Amer. Assoc. Cancer Res., 88th Annual Meeting, April 12-16, San Diego, CA, (abstract 3856) 38:574(1997).

Publications

1.Brown, K.L. and Hakimi, J.M. 31P NMR Observation of Phosphodiester Protonation of the Nucleotide Loop of Methylcobalamin. Inorganica Chimica Acta. 67: L29-31 (1982).

2.Brown, K.L., Hakimi, J.M. and Marynick, D.S. 13C and 31P NMR Studies of 13CN-Cyanocobalamine in Sulfuric Acid-Water Mixtures. Inorganica Chimica Acta. 79:120-123 (1983).

3.Brown, K.L., Hakimi, J.M., Nuss, D.M., Montejano, Y.D. and Jocobsen, D.W. Acid-Base Properties of Alpha-Ribazole and the Thermodynamics of Dimethylbenzimidazole Association in Alkylcobalamin. Inorg. Chem. 23:1463-1471 (1984).

4.Brown, K.L. and Hakimi, J.M. Heteronuclear NMR Studies of Cobalamins. 2. 13C and 31P NMR Studies of 13CN-Cyanocobalamin. Inorg. Chem. 23: 1756-1764 (1984).

5.Brown, K.L., Hakimi, J.M., and Jacobsen, D.W. Heteronuclear NMR Studies of Cobalamins. 3. 31P NMR of Aquocobalamin and Various Organocobalamins. J. Am. Chem. Soc. 106:7894-7899 (1984).

6.Brown, K.L., Hakimi, J.M. and Huang, Y.J. Substituent Effect of Chelated Cobalt. 8. Hyperconjugation in Carboxyalkylcobalamins. Inorganica Chimica Acta. 106:123-128 (1985).

7.Brown, K.L., Hakimi, J.M. Heteronuclear NMR Studies of Cobalamins. 4. Alpha-Ribazole-3’-Phosphate and Nucleotide Loop of Base-On Cobalamins. J. Am. Chem. Soc. 108:496-503 (1986).

8.Hakimi, J.M., Waldman, A.S., and Scocca, J.J. Site-Specific Recombination between Cloned attP and attB Sites from the Haemophilus influenzae Bacteriophage HP1 Propagated in Recombination-Deficient Escherichia coli. J. Bacteriol. 171:1747 –1750 (1989).

9.Hakimi, J.M. and Scocca, J.J. Binding Sites for Bacteriophage HP1 Integrase on its DNA Substrates. J. Biol. Chem. 269:213**-***** (1994).

10.Hakimi, J.M. and Scocca, J.J. Purification and Characterization of the Integrase from the Haemophilus influenzae bacteriophage HP1; Identification of a Four-Stranded Intermediate and the Order of Strand Exchange. Molec. Microbiol. 21:147-158 (1996).

11.Schoenberg, M.P., Hakimi, J.M., Wang, S., Bova, G.S., Epstein, J.I., Fischbeck, K.H., Isaacs, W.B., Walsh, P.C., Barrack, E.R. Microsatellite Mutation (CAG24-18) in the Androgen Receptor Gene in Human Prostate Cancer. Biochem. Biophys. Res. Commun. 198:74-80 (1994).

12.Hakimi, J.M., Rondinelli, R.H., Schoenberg, M.P. and Barrack, E.R. Androgen Receptor Gene Structure and Function in Prostate Cancer. World J. Urol. 14: 329-337 (1996).

13.Hakimi, J.M., Rondinelli, R.H., Schoenberg, M.P. and Barrack, E.R. Androgen Receptors in Human Prostate Cancer: Heterogeneous Expression, Gene Mutations, and Polymorphic Variants. In: Hormones and Cancer (W.V. Vedeckis, Ed.), Birkhauser, Boston, pp. 445-492 (1996).

14.Hakimi, J.M., Schoenberg, M.P., Rondinelli, R.H., Piantadosi, S., and Barrack, E.R. Androgen Receptor Variants with Short Glutamine or Glycine Repeats Identify Unique Subpopulation of Men with Prostate Cancer. Clinical Cancer Research 3:1599-1608 (1997).

Contact this candidate