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Asho Ali (Ph.D.)

***** ******* ******* **********, ** 20148

440-***-**** adwyio@r.postjobfree.com

https://www.linkedin.com/in/asho-ali-92b0021b/

SUMMARY STATEMENT

Possess extensive research and teaching experience in the area of Microbiology and Molecular Biology. Have wide experience of working with infectious microbes in Bio-safety level-II and III facilities in research and clinical laboratories following site specific Standard Operating Procedures (SOP) and instructions. Competent in using various molecular and microbial methods, including culture, Drug susceptibility testing, Tissue culture, DNA and RNA extraction, Conventional PCR, Real-time PCR, Probe based PCR, VNTR typing, RFLP, Gene sequencing etc. Besides, have extensive teaching experience of Microbiology, Biological sciences, and facilitation of lab for diverse body of students at international universities in USA, Saudi Arabia and Pakistan. Have strong background of performing collaborative, interdisciplinary research and teaching with dedication, teamwork, and high work ethics. Recipient of three outstanding teaching awards. EDUCATION

-Ph.D. in Microbiology from Aga Khan University (AKU), Pakistan (2002- 2009)

-M.Sc. in Microbiology from Karachi University, Pakistan (1986-1989)

-B.Sc. with Microbiology, Chemistry and Zoology from Karachi University, Pakistan

(1984-1986)

WORK EXPERIENCE

Molecular Laboratory Scientist: December 2019- to date Microbiology Laboratory, Nationwide Children’s Hospital, Columbus, USA Major responsibilities include detection of microbes in clinical samples using molecular methods. Also, preparing and reporting results using various Laboratory Information and Management Systems. In addition, taking care of reagents and supplies for smooth functioning of lab. Also, maintaining inventory of supplies, reagents, and chemicals.

Adjunct faculty, Microbiology: December. 2019- to date Felbry College of Nursing, Columbus, USA

Major responsibilities include teaching Microbiology course (Theory/laboratory), participating in college faculty meeting, advising students.

Microbiology Scientist II: May 2019 – October 2019 Ohio Dept. of Health, Columbus, USA

Major responsibilities include isolation and identification of Virus in samples received from various Ohio counties using Molecular methods.

Associate Professor: Feb. 2014 - Sept. 2017

Department of Biology, Science College for Girls

King Abdulaziz University, Jeddah, KSA

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Major responsibilities included teaching Biological Science courses to graduate and under-graduate students, participating in college/university committees, and researching and advising students’ theses. Assistant Professor: July 2007 - Nov. 2013

Dept. of Pathology& Microbiology, and School of Nursing Major responsibilities included teaching Biological Science courses to graduate and under-graduate students, participating in college/university committees, and researching and advising students’ theses. Instructor and Sr. Instructor: August 1990 – June 2007 Science Section, Aga Khan University, School of Nursing, Karachi, Pakistan Major responsibilities included teaching Biological Science courses to under-graduate students, such as Microbiology, Life Sciences, Applied Sciences, and Biostatistics. Also, participated in standing committees, such as Admission and Progression, Appointment and promotion, Disciplinary, coordinating administrative tasks of assigned academic programs in the department, such as Orientation, Diploma and Track-I Assistant Technologist, August 1989 – July 1990

Science Section, Aga Khan University, School of Nursing, Karachi, Pakistan Major responsibilities included developing and trying out of new laboratory experiments for science courses including Microbiology, Chemistry and Biophysics, conducting laboratory-teaching sessions, organizing and setting up the laboratory, ordering equipment and chemicals, and maintaining inventory. PUBLICATIONS

>20 articles in internationally reputed journals, selected articles are listed below:

• Akbar Kanji, Rumina Hasan, Asho Ali, Ambreen Zaver, Ying Zhang et al “Single nucleotide polymorphisms in efflux pumps genes in extensively drug resistant Mycobacterium tuberculosis isolates from Pakistan” Tuberculosis, Vol 107: (12), 2017, 20-30

• Akbar Kanji, Rumina Hasan, Ambreen Zaver, Asho Ali et al “Alternate efflux pump mechanism may contribute to drug resistance in extensively drug-resistant isolates of Mycobacterium tuberculosis” Int J of Mycobacteriology, 5 (2016). S97-S98

• Ruaa A. Lahzah, Asho Ali “Association of Phenotypic and Genotypic Fluoroquinolone Susceptibility among NonTyphoidal Salmonella Isolates from Jeddah, KSA” Journal of Biosciences and Medicines, 2016, 4, 100-109

• Sehrish Sajjad, Asho Ali, Raisa B. Gul, Ahmed Mateen, Shafquat Rozi “The Effect of individualized patient education with emotional support on the quality of life of breast cancer patients-A pilot study”. Eu. J of Oncology Nursing 21 (1): 2016, 75-82

• Ali A, Hasan Z, Ruth McNerney, Kim Mallard, Francesc Coll, Grant Hill-hawthorne, Mridul Nair, Arnab Pain, Taane Clark and Rumina Hasan.“ Whole Genome Sequencing Based Characterization of Extensive Drug resistant Mycobacterium tuberculosis isolates from Pakistan.” PLoS One 10 (2): 2015

• Ali A, Hasan Z, Jafri S, Inayat R, Hasan R. Mycobacterium tuberculosis Central Asian Strain (CAS) lineage in Pakistan reveal lower diversity of MIRU loci than other strains. Int J of Mycobacteriology 3

(2014) 108 – 116

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Akbar Kanji, Zahra Hasan, Asho Ali, Ruth McNerney, Kim Mallard, Francesc Coll, Grant Hill- Cawthorne, Arnab Pain, Mridul Nair, Taane Clark, Ambreen Zaver, Sana Jafri, and Rumina Hasan. Characterization of genomic variations in SNPs of PE_PGRS genes reveals deletions and insertions in extensively drug resistant (XDR) M.tuberculosis strains from Pakistan. Int J of Mycobacteriology 4

(2015) 73– 79

• Ali A “Mycobacterium tuberculosis and Molecular Epidemiology: An Overview.” Journal of Microbiology Research 2014, 4(6A): 25-31

• Ali A, Hasan R, Jabeen K, Jabeen N, Qadeer E and Hasan Z. Characterization of mutations conferring extensive drug resistance to M.tuberculosis isolates in Pakistan. Antimicrob. Agents Chemother; Vol.55, No. 12, December 2011. (Impact Factor 4.841)

• Hasan R, Jabeen K, Ali A, Rafiq Y, Laiq R, Malik B, et al. Extensively Drug-Resistant Tuberculosis, Pakistan. Emerging Infectious Diseases Vol. 16, No. 9, September 2010. (Impact Factor 6.169)

• Ali A, Hasan Z, Moatter T, Tanveer M, Hasan R. M. tuberculosis Central Asian Strain 1 MDR isolates have more mutations in rpoB and katG genes compared with other genotypes. Scand J Infect Dis 2009;41(1):37-44.

• Tanveer M, Hasan Z, Siddiqui AR, Ali A, Kanji A, Ghebremicheal S, et al. Genotyping and drug resistance patterns of M. tuberculosis strains in Pakistan. BMC Infect Dis 2008; 8:171. (Impact Factor 3.12)

• Ali A, Hasan Z, Tanveer M, Siddiqui AR, Ghebremichael S, Kallenius G, et al. Characterization of Mycobacterium tuberculosis Central Asian Strain 1 using mycobacterial interspersed repetitive unit genotyping. BMC Microbiol 2007;7:76. (Impact Factor 3.04) GRANTS AWARDED

Recipient of three research grants for the following studies:

-Fluoroquinolone resistance; role of gene mutations and efflux pump among clinical isolates of Mycobacterium tuberculosis

- Association of drug resistance Gene Mutations with Genotyping of M. tuberculosis strains from Pakistan

- To characterize drug resistant mutations in XDR- MTB strains prevalent in Pakistan and development of rapid assays for drug resistance screening AWARDS AND HONOR

1. Received Bursary from Welcome Trust to attend a course on “Genomics and Clinical Microbiology” in 2011

2. Received funding from WHO and TDR to attend course on “Bioinformatics in Infectious Diseases” in 2002

3. Recipient of two “Teacher of the Year” and one “Outstanding Classroom Teacher” award in 1996 at the Aga Khan University

REFERENCES

-Available on request

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