Aikaterini Pringa

Biotech/Operations and Manufacturing Manager

Aikaterini Pringa

*** ********** ***,

Boston, MA 02131

617-***-****

adwsx5@r.postjobfree.com

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Management Skills

A senior biotech research leader, and manufacturing innovator who is piloting healthcare-centric roadmaps, empowering independence, and inspiring the next generation of enterprise growth. A scientific visionary who serves as a catalyst for sustainable change by empowering infrastructure/ operations/manufacturing teams through data-driven impact. A driver of continuous improvement who is rethinking what's possible, transforming complex obstacles into tangible and profitable solutions.

Solutions Based Leadership, Collaborative Innovation, Critical Thinking and Creative Problem Solving, Analytical and Detailed Focused, Performance Management (Training / Leadership / Mentorship), Cross-Functional Team Leadership, Change Management, Communication, Accountability, Strategic Planning and Innovation, Goal Setting, Inclusive Leadership, Compliance, Operational Assessment, Time Management, Multitasking, Influence, Resilience, Integrity, Conflict Resolution, Continual Process Improvement, Emotional and Cultural Intelligence, Flexibility / Adaptability

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Experience

Ginkgo Bioworks / Growth Media Manager

2018 - 2022, South Boston

●Spearheads growth media program leadership; leads 7 team members in meeting all bacterial and fungal growth media deadlines for all 3 Ginkgo laboratories across MA, supporting 900 employees with stocked and customized growth media on demand. Organizing/overseeing growth media shipping.

●Owns on-demand 800+ customized growth media, 48-hour deadline; provides customer support 7 days per week.

●Created and implemented the media submission files resulting in up to 200% increased delivery accuracy, boosting customer satisfaction.

●Optimizes and transforms the corporate media budget through cost containment strategies driving growth and profitability measures in liaison with the financial department/procurement.

●Cross functionally collaborates with aligned staff in other functions such as finance, safety, lab operations, core facilities, QA/QC departments, and research groups; develops product market prices and cost estimates.

●Shapes and supports continued expansion with stakeholders, leads dry run trails, communicates needed changes and strategic plans; optimizes growth media product protocols.

●Publishes and shapes a growth media home page, monthly media usage reports, media production database, generated and constantly updates a custom growth media catalog, authors SOPs; presents KPIs to stakeholders.

●Hires, trains, and mentors staff members through leading and influencing, improving employee satisfaction, and dramatically reducing turnover; champion procurement and general lab management teams to meet their deadlines. Trained/supervised for more than a year a general lab support team of 2.

●Transformed staffing levels by 200 % as a function of unprecedented company growth with measurable productivity metrics.

●Authors and conceptualizes all training and development for multiple staff members as well as administering all annual performance reviews; mentors high-potential leaders, enabling promotions of team members.

Boston Children's Hospital / Lab Manager

2017 - 2018, Boston

●Orchestrated complete laboratory management; led six global research investigators and two research assistants in conceptualizing and performing experiments studying thrombosis, wound healing, and inflammation.

●Authored documentation for several colonies of genetically engineered mice, and isolated and analyzed cells from human and mouse blood samples.

●Orchestrated the successful project management for laboratory relocation; cross-functionally collaborated with facilities and safety personnel, architects, equipment supplier, and moving companies.

●Coached, trained, and mentored global team members; shaped and supported recruitment and selection with human resources.

●Piloted federal and company grants and liaised with the finance department.

Boston University, Dental School / Lab manager

2012 - 2017, Boston

●Mentored dental doctoral candidates in cellular techniques; designed and supervised their experiments, data analysis, and proctored exams.

●Collaborated with dental surgeons coordinating dental samples laboratory transportation; isolated primary human osteoblasts and pulp cells.

●Conducted experiments studying cell proliferation and differentiation using fluorometry, immunosorbent assay, and mineralization straining.

●Authored scientific manuscripts and abstracts, supported thesis writing, conducted tours, welcomed new students/parents, and shaped the presentation of data at international scientific conferences.

●Oversaw financial analysis of the laboratory as well as safety compliance.

Tufts Medical Center / Research Associate II

2008 - 2012, Boston

●Studied isoform expression of the receptor tyrosine -protein kinase ErbB4, known to be expressed in the lung during mouse lung development maturation to understand if individual isoforms are driving proliferation and/or differentiation events; investigated if changes in the ErbB4 isoforms were involved in the prenatal lung development, comparing the isoforms expression pattern in premature and primary mouse fetal alveolar Type II cells.

●Performed studies on cellular proliferation and differentiation by overexpressing each ErbB4 variant in MLE12.

●Mass General Hospital/ Research Associate I

2006 - 2008 Boston

●Engineered a K.lactis clone to establish an expression system that could produce post-translationally fucosylated recombinant milk components with the idea of using it to make useful and sizable industrial-scale milk components containing the right post-translational modification.

Rhode Island Hospital / Research Associate I

2004 - 2006 Providence

●Kv2.1 is voltage-gated potassium (Kv) channel α subunit expressed in mammalian atria heart and ventricle. During cardiac hypertrophy, myocardial infarction, and hypothyroidism, the channel expression is reduced. At that time, we wondered if this reduction in expression and functionality could be due to the failure of the subunits to tetramerize to a functional channel. I studied tetramerization assembly of the potassium voltage-gated channel Kv2.1 by mutating the four identical subunits' zinc-binding amino acidic residues, expressing the mutants in rabbit reticulocytes, and fractionating the translated products by site exclusion chromatography checking assembly status. Transfected the mutants in Chinese hamster ovary cells (CHO) known to express no endogenous Kv21 and studied cellular localization/trafficking of the variants using fluorescent microscopy. I tested channel functionality employing a whole-cell patch-clamp technique in collaboration with lab electrophysiologists.

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Education

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Technical Skills

University of Hamburg, Germany

Doctor in Philosophy, PhD

●Molecular cloning and mutagenesis, DNA purification, PCR, Southern blot.

●RNA extraction, cDNA synthesis, semi-quantitative RT-PCR, Northern Blot, TaqMan qRT-PCR

●Protein expression in coli, K. lactis, rabbit reticulocytes lysates and various established cell lines, Purification, Western Blotting, Co-Immunoprecipitation, ELISA, Gel filtration, IF microscopy

●Isolation of primary human (teeth, bone) and mouse cell cultures, gene targeting in mouse embryonic stem cells.

●Mouse colony handling and expansion, Mouse ear and tail sampling, mouse and rabbit genotyping.

●Organ isolation from mice/rat and sample preparation for RNA, DNA and protein extraction.

●Luciferase promoter assays, Multi toxicity assays, Cell viability assays, proliferation studies, FACS

●Lipid isolation from membrane fractions, Thin layer chromatography

●Transient and stable cell line transfections

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Publications/Abstracts

1.Lu. WC., Pringa. E ., Chou. LC. Effect of Magnesium on the osteogenesis of normal human osteoblasts, 2017 Magnesium research, Vol 30 (2): 42-52. DOI : 10.1684/mrh.2017.0422

2.Brunner. M., Peng. X., Liu. GX., Ren. XQ., Mathur. R., Hajjiri. M., Rana. N.E., Steinberg, E., Folco. E., Pringa. E., Centracchio. J., Macharzina. R., Donahay. T., Schofield. L., Odening. K., Kirk. M, . Mitchell. G., Poppas. A., Zehender.M., Koren. G., Human KvLQT1-Y315S and HERG-G628S Cause a Long QT Phenotype and Sudden Cardiac Death in Rabbits, 2008 Journal of Clinical Investigation 118:2246-59.

3.Pringa. E., Martinez-Noel. G., Mueller. U., Harbers. K. Interaction of the RING finger-related U-box motif of a nuclear dot protein with ubiquitin- conjugating enzymes, 2001 J. Biol. Chem 276: 19617-23.

4.Pringa. E., Meier. I., Mueller. U., Martinez-Noel. G., Harbers. K., Disruption of the gene encoding the ubiquitin-conjugating enzyme UbcM4 has no effect on proliferation and in vitro differentiation of mouse embryonic stem cells, 2000 Biochim Biophys Acta 1494: 75-82.

5.Ritzkat. A., Pringa. E., Scapin. C., Guengoeze. O., Dere. M., Volpe. MA., Dammann. C.E.L., Nielsen. H.C. Presenillin-1 is crucial for surfactant Protein B and C mRNA expression in Murine MLE-12 cells. May 2010 Conference Paper. DOI: 10.1164/ajrccm-conference.2010.181.1_MeetingAbstracts.A2461

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