Research Associate Medical Officer

Location:

Mercer Island, WA

Posted:

May 12, 2023

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Resume:

CURRICULUM VITAE

Xin Wu

OncoResponse • **** Columbia Street Suite 300 Seattle WA • Cell 614-***-**** • *********@*****.*** QUALIFICATION SUMMARY

Both academia and industry experience with strong cancer and immunology knowledge and practical bench work experience

Adept in project design, result analysis and problem solving

Expert in molecular biology and virus-based gene therapy

Extensive experience in cancer biomarker screening and related cell signaling pathway analysis

Experience in animal husbandry including knock-in and knock-out mice colony maintenance and histology study.

Skilled in immunotyping different immunological cell populations from various types of human tissues including whole blood, bone marrow, cerebrospinal fluid, ascites and cancer tissues using multicolor flow cytometry and FlowJo

Design gene functional study using siRNA and CRISPR-Cas9 (both knock-in and knock-out)

Antibody protein purification using AKTA Explorer, Avant and Pure, Agilent 1260 Infinity HPLC as well as Waters ACQUITY Premier UPLC System

Experience in bioinformatic analysis including TCGA dataset, microarray, RNA-seq, Linux, R, C++ programming

WORKING EXPERIENCE

January 2021 – present Associate Scientist Oncoresponse LLC

Characterize antibody specificity by flow cytometry, ELISA, alphaLISA, octet RH16

Establish stable gene expression cell line using flow cytometry

Characterize biophysical property of antibody using DSF, IEF, endotoxin quantification, reducing and non-reducing SDS PAGE

Protein-protein interaction using magnetic beads immunoprecipitation and epitope mapping using Western blot

CRISPR gene knockout Fut8 gene

Construct expression vectors and use HEK293 6e and EpiCHO cells for monoclonal antibody production

Small scale monoclonal antibody production and purification using AKTA, and UPLC System November 2018 – October 2019 Technologist Bloodworks Northwest, Seattle, WA

Analysis of CD34 stem cells in whole blood and apheresis of autologous transplant cancer patients as well as cord blood by flow cytometry

Establish the protocol of lymphocytes analysis using CD3/CD4/CD8/CD45 by flow cytometry

Diagnose hematological diseases such as leukemia and lymphoma using flow cytometry January 2015 – March 2017. Postdoctoral Fellow, Comprehensive Cancer Center, The Ohio State University, Columbus, OH. PI: Dr. Takeshi Kurita

Explore the interaction between tumor and its microenvironment in human uterus leiomyomas

Study the leiomyoma growth under the regulation of hormone by PDTX mouse model

Establish MED12 knock-in mouse model by CRISPR to study the leiomyoma growth regulation October 2009 – January 2015. Research Associate, Comprehensive Cancer Center, The Ohio State University, Columbus, OH. PI: Dr. Gregory Otterson

Explore PRMT5 related signaling pathways in drug resistance in lung cancer chemotherapy 2

Explore the effect of small molecules targeting PRMT5 as a new class of drug in treating lung cancer, GBM and hematologic malignancies

Determine the effect of microRNA on lung cancer pathogenesis October 2004 – October 2009. Postdoctoral Researcher, Comprehensive Cancer Center, The Ohio State University, Columbus, OH. PI: Dr. Gregory Otterson

Identify microRNA biomarkers for the diagnosis and treatment of lung cancer

Design in vivo system to screen the microRNA targets

Determine the effect of drugs on the microRNA profiling October 1996 – September 2004. Graduate Fellow and Investigator, Liver Cancer Institute, Fudan University, Shanghai, China

Identify new tumor suppressor gene on chromosome 8p in human liver cancer

Establish a new human primary liver cancer cell line

Supervise the laboratory and provide the experimental instruction for the graduate students and visiting scholars

EDUCATION

Ph.D, Biochemistry & Molecular biology. Fudan University, Shanghai, China. January 2003

Dissertation: “Screening Candidate Tumor Suppressor Gene on Chromosome 8p in Human Hepatocellular Carcinoma”

MS, Biochemistry. Shanghai Medical University, Shanghai, China. July 1996

Dissertation: “Effects on the cytokine expression of mouse macrophages by exogenous gangliosides”

MD, Clinical Medicine. Bengbu Medical College, Bengbu, Anhui, China. July 1991 CONTINUED EDUCATION

Department of Computer and Information Science. The Ohio State University. Sep. 2010- July 2015 Computer Science major (undergraduate)

CERTIFICATE

AABB Cellular Therapies Certificate Program 2020

Integrative Statistical Analysis of Genome Scale Data. Cold Spring Harbor laboratory, NY, 2009 PUBLICATIONS

1. Xin Wu, Vanida Serna, Justin Thomas, Wenan Qiang, Michael Blumenfeld, and Takeshi Kurita. Subtype- specific contribution of tumor-associated fibroblasts to the pathogenesis of uterine leiomyoma. Cancer Research. 2017 Dec 15;77(24):6891-6901.

2. Vanida Serna, Xin Wu, Justin Thomas, Wenan Qiang, Michael Blumenfeld, and Takeshi Kurita. Cellular kinetics of MED12 mutant uterine leiomyoma growth and regression in vivo. Endocrine-Related Cancer. 2018 Jul;25(7):747-759. .

3. Yan F, Alinari L, Lustberg ME, Martin LK, Cordero-Nieves HM, Banasavadi-Siddegowda Y, Virk S, Barnholtz-Sloan J, Bell EH, Wojton J, Jacob NK, Chakravarti A, Nowicki MO, Wu X, Lapalombella R, Datta J, Yu B, Gordon K, Haseley A, Patton JT, Smith PL, Ryu J, Zhang X, Mo X, Marcucci G, Nuovo G, Kwon CH, Byrd JC, Chiocca EA, Li C, Sif S, Jacob S, Lawler S, Kaur B, Baiocchi RA. Genetic validation of the Protein Arginine Methyltransferase PRMT5 as a candidate therapeutic target in glioblastoma. Cancer Res. 2014;74(6):1752-65.

4. Shilo K, Wu X, Sharma S, Welliver M, Duan W, Villalona-Calero M, Fukuoka J, Sif S, Baiocchi R, Hitchcock CL, Zhao W, Otterson GA. Cellular localization of protein arginine methyltransferase-5 correlates with grade of lung tumors. Diagn Pathol. 2013;8:201. 5. Nicholas C, Yang J, Peters SB, Bill MA, Baiocchi RA, Yan F, Sïf S, Tae S, Gaudio E, Wu X, Grever MR, Young GS, Lesinski GB. PRMT5 is upregulated in malignant and metastatic melanoma and regulates expression of MITF and p27 (Kip1). PLoS One. 2013;8(9):e74710. 6. Duan W, Gao L, Zhao W, Leon M, Sadee W, Webb A, Resnick K, Wu X, Ramaswamy B, Cohn DE, Shapiro C, Andreassen PR, Otterson GA, Villalona-Calero MA. Assessment of FANCD2 nuclear foci formation in paraffin-embedded tumors: a potential patient-enrichment strategy for treatment with DNA interstrand crosslinking agents. Transl Res. 2013;161(3):156-64. 7. Mortazavi A, Ling Y, Martin LK, Wei L, Phelps MA, Liu Z, Harper EJ, Ivy SP, Wu X, Zhou BS, Liu X, Deam D, Monk JP, Hicks WJ, Yen Y, Otterson GA, Grever MR, Bekaii-Saab T. A phase I study of prolonged infusion of triapine in combination with fixed dose rate gemcitabine in patients with advanced solid tumors. Invest New Drugs. 2013;31(3):685-95. 8. Martin LK, Grecula J, Jia G, Wei L, Yang X, Otterson GA, Wu X, Harper E, Kefauver C, Zhou BS, Yen Y, Bloomston M, Knopp M, Ivy SP, Grever M, Bekaii-Saab T. A dose escalation and pharmacodynamic study of triapine and radiation in patients with locally advanced pancreas cancer. Int J Radiat Oncol Biol Phys. 2012;84(4):e475-81.

9. Yan F, Wu X, Crawford M, Duan W, Wilding EE, Gao L, Nana-Sinkam SP, Villalona-Calero MA, Baiocchi RA, Otterson GA.The search for an optimal DNA, RNA, and protein detection by in situ hybridization, immunohistochemistry, and solution-based methods. Methods. 2010; 52(4):281-6. 10. Nuovo GJ, Wu X, Volinia S, Yan F, di Leva G, Chin N, Nicol AF, Jiang J, Otterson G, Schmittgen TD, Croce C. Strong inverse correlation between microRNA-125b and human papillomavirus DNA in productive infection. Diagn Mol Pathol. 2010;19(3):135-43. 11. Duan W, Gao L, Wu X, Wang L, Nana-Sinkam SP, Otterson GA, Villalona-Calero MA. MicroRNA-34a is an important component of PRIMA-1 induced apoptotic network in human lung cancer cells. Int J Cancer. 2010; 127(2):313-20.Kosuri KV, Wu X, Wang L, Villalona-Calero MA, Otterson GA. An epigenetic mechanism for capecitabine resistance in mesothelioma. Biochem Biophys Res Commun. 2010; 391 (3) :1465-70.

12. Wu X, Piper-Hunter MG, Crawford M, Nuovo GJ, Marsh CB, Otterson GA, Nana-Sinkam SP. MicroRNAs in the Pathogenesis of Lung Cancer. Journal of Thoracic Oncology. 2009; (8):1028-34. 13. Crawford M, Batte K, Yu L, Wu X, Nuovo GJ, Marsh CB, Otterson GA, Nana-Sinkam SP. MicroRNA 133B targets pro-survival molecules MCL-1 and BCL2L2 in lung cancer. Biochem Biophys Res Commun. 2009;388(3):483-9.

14. Duan W, Gao L, Wu X, Hade EM, Gao JX, Ding H, Barsky SH, Otterson GA, Villalona-Calero MA. Expression of a mutant p53 results in an age-related demographic shift in spontaneous lung tumor formation in transgenic mice. PLoS One. 2009;4(5):e5563. 15. Chai G, Li L, Zhou W, Wu L, Zhao Y, Wang D, Lu S, Yu Y, Wang H, McNutt MA, Hu YG, Chen Y, Yang Y, Wu X, Otterson GA, Zhu WG. HDAC inhibitors act with 5-aza-2'-deoxycytidine to inhibit cell proliferation by suppressing removal of incorporated abases in lung cancer cells. PLoS ONE. 2008;3(6):e2445

16. Wu X, Jia HL, Wang YF, Ren N, Ye QH, Sun HC, Wang L, Liu YK, Tang ZY, Qin LX. HTPAP gene on chromosome 8p is a candidate metastasis suppressor for human hepatocellular carcinoma. Oncogene. 2006; 25(12):1832-40

17. Duan W, Gao L, Wu X, Zhang Y, Otterson GA, Villalona-Calero MA. Differential response between the p53 ubiquitin-protein ligases Pirh2 and MdM2 following DNA damage in human cancer cells. Exp Cell Res. 2006;312(17):3370-8.

18. Zhang H, Ye QH, Ren N, Zhao L, Wang YF, Wu X, Sun HC, Wang L, Zhang BH, Liu YK, Tang ZY, Qin LX. The prognostic significance of preoperative plasma levels of osteopontin in patients with hepatocellular carcinoma. J Cancer Res Clin Oncol. 2006;132(11):709-17. 19. Hui L, Zhang X, Wu X, Lin Z, Wang Q, Li Y, Hu G. Identification of alternatively spliced mRNA variants related to cancers by genome-wide ESTs alignment. Oncogene. 2004; 23 (17):3013-23 4

20. Li Y, Tian B, Yang J, Zhao L, Wu X, Ye SL, Liu YK, Tang ZY. Stepwise metastatic human hepatocellular carcinoma cell model system with multiple metastatic potentials established through consecutive in vivo selection and studies on metastatic characteristics. J Cancer Res Clin Oncol. 2004;130(8):460-8. 21. Zhao L, Qin LX, Ye QH, Zhu XQ, Zhang H, Wu X, Chen J, Liu YK, Tang ZY. KIAA0008 gene is associated with invasive phenotype of human hepatocellular carcinoma--a functional analysis. J Cancer Res Clin Oncol. 2004;130 (12):719-27.

22. Jiang Y, Zhou XD, Liu YK, Huang XW, Zhao Y, Xue Q, Sun RX, Chen J, Wu X. Antisense Tcf inhibits the neoplastic growth of liver cancer cells. J Cancer Res Clin Oncol. 2004;130(11):671-8 23. Liang CM; Zhong CP; Zheng N; Wu X; Sun RX; Liu YK; Ye SL To construct a recombinant adeno- associated virus vector carrying the gene encoding the mature peptide of secondary lymphoid-tissue chemokine. Acta Anatomica Sinica, 2004; 35(2):147-51 24. Wu X, Jia HL, Liu YK, Li Y, Chen J, Zhao Y, Yu L, Qin LX, Tang ZY. Comparative study on gene expression difference between high and low metastatic human hepatocellular carcinoma cell strains. China Oncology, 2003(2); 13:97-105

25. Huang R, Xing Z, Luan Z, Wu T, Wu X, Hu G. A specific splicing variant of SVH, a novel human armadillo repeat protein, is up-regulated in hepatocellular carcinomas. Cancer Res. 2003; 63(13): 3775- 82

26. Zhang LH, Qin LX, Ma ZC, Ye SL, Liu YK, Ye QH, Wu X, Huang W, Tang ZY. Allelic imbalance regions on chromosomes 8p, 17p and 19p related to metastasis of hepatocellular carcinoma: comparison between matched primary and metastatic lesions in 22 patients by genome-wide microsatellite analysis. J Cancer Res Clin Oncol. 2003;129:279-86

27. Jiang Y, Zhou XD, Liu YK, Wu X, Huang XW. Association of hTcf-4 gene expression and mutation with clinicopathological characteristics of hepatocellular carcinoma. World J Gastroenterol, 2002;8: 804-7 28. Wang YC, Chen ZR, Chen ZW, Guan XQ, Song HY, Wu X, Liu YK. Recombinant human heparin binding neurite promoting factor expressed with yeast stimulates neuritis outgrowth. Chinical Medical J, 2002; 115(9):1352-7

29. Jiang Y, Zhou XD, Liu YK, Wu X. T cell factor 4 gene expression and mutation in hepatocellular carcinoma. China Oncology, 2002; 12(1): 5-8

30. Jiang Y, Zhou XD, Xue Q, Liu YK, Wu X. Relationship between hTcf-4 gene expression and liver cancer metastasis. Shaanxi Oncology Medicine, 2002; 10 (2): 88-90 31. Jiang Y, Zhou XD, Liu YK, Wu X. Study on the expression and mutation of hTcf-4 gene in liver cancer cell lines. Chinese Journal of Experimental Surgery, 2002; 19 (2): 107-9 32. Xu L, Hui L, Wang S, Gong J, Jin Y, Wang Y, Ji Y, Wu X, Han Z, Hu G. Expression profiling suggested a regulatory role of liver-enriched transcription factors in human hepatocellular carcinoma. Cancer Res. 2001 Apr 1; 61(7):3176-81

33. Wang YC, Chen ZR, Chen ZW, Gong XQ, Song HY, Wu X, Liu YK. Recombinant human heparin- binding neurite-promoting factor expressed with yeast and its neurite-promoting activity. Chinese Journal of Traumatology, 2001; 17(12):740-4

34. Zhang W, Zhou JP, Liu YK, Wu X. Expression of leukemia inhibitory factor mRNA and its localization in the endometrium of early pregnant mouse. J. Shanghai Medical University, 2000;27(4):280-2 35. Zhang JH, Wu JP, Liu YK, Zhou JP, Wu X. Changes of Endometrial β_3 Integrin during Early Pregnancy. Reproduction and Contraception, 2000; 20(3):151-5

36. Liu YK, Zhang CD, He B, Wu X, Ye SL, Tang ZY. E-selectin mediates early adhesion of HepG2 hepatoma cells to endothelial cells. Chin J Oncol, 2000; 22(6):449-52 37. Wu X, Liu YK, Zhang XY. Effects on the cytokine expression of mouse macrophages by exogenous gangliosides. China Oncology, 1999; 9(2):138-140

38. Jiang YA, Wu X, Liu YK. The detection of telomerase activity in hepatocarcinoma. China Oncology, 1999; 9(4): 272-5

39. Zhu J, Zhou JP, Liu YK, Wu X. Expression of Intercellular Adhesion Molecule 1 mRNA In the Mouse's Endometrium During Implantation Periods. Journal of Shanghai Medical University, 1999; 26(5): 365-7 40. Wu X, Liu YK. Effect of ganglioside GM3 and GD3 on the cytotoxicity of mouse macrophage. Tumor 1998; 18(5):334-336

41. He B, Liu YK, Tang ZY, Wu X, Zhang Y. Clinical significance of expressions of osteopontin in hepatocellular carcinoma. Chin J Hepatol, 1998;6(4):208-10 5

42. Zhu J, Zhou JP, Liu YK, Wu X, Zhang JH. The expression of protooncogene c-jun, c-fos in the mouse endometrium during the periimplantation and postimplantation period. Journal of reproductive medicine. 1998; 7(4): 231-4

43. Zhu J, Zhou JP, Liu YK, Wu X, Zhang JH. The expression of protooncogene c-jun, c-fos in the human early pregnant chorion and decidua. Chin J Obstet Gynecol, 1998; 33(4): 240-1 44. Wu JP, Zhang JH, Liu YK, Wu X, Zhou JP. The Expression of Fibronetin in Endometrium before and after Embryo Implantation. Reproduction and Contraception, 1998; 18 (4) : 205-9 Book Chapters

Lunxiu Qin, Xin Wu et al. Molecular Diagnosis and prediction of Cancer. Shanghai Scientific & Technological Education Press, China, 2004

JOURNAL REVIEWER

Editorial Board. Gastroenterology Research. 2010

Editorial Board. The Internet Journal of Oncology. 2010

Invited Reviewer. Methods. 2010

Reviewer of The American Journal of Pathology

Cell Biology International

Journal of Experimental & Clinical Cancer Research Cancer Research

Modern Pathology

Diagnostic Molecular Pathology

Journal of Hematology & Oncology

Journal of Carcinogenesis

Journal of RNAi and Gene Silencing

Gene Regulation and Systems Biology

Radiation Oncology

Oncotarget

TECHNICAL SKILLS

Molecular biology: PCR-Select cDNA Subtraction, Yeast two-hybrid, RACE-PCR, Real time PCR, MS- PCR, RT-PCR, Mutagenesis, Southern blotting, cDNA microarray, cDNA library construction, Northern blotting, Adeno-associated Virus (AAV), Adenovirus (AV), Lentivirus and Retrovirus construction, virus titration and infection, siRNAs and miRNA vector construction and transfection, DNA methylation, Recombinant protein expression in E. coli, Luciferase assay, In situ hybridization Protein biochemistry & Immunology: Humanized monoclonal antibody production and protein purification using AKTA Explorer, Avant and Pure, Agilent 1260 Infinity HPLC as well as Waters ACQUITY Premier UPLC System, immunoprecipitation (IP), Western blotting, ELISA, Immunohistochemistry (include immunofluorescence)

Cell biology: Cell cycle, apoptosis analysis and cell sorting by FACS, multichannel flow cytometry for HSCT and hematology malignant diagnosis, Confocal microscopy, Fluorescence microscopy, cell migration assay, Colony forming assay, MTT,

Animal experiment: Zinc finger nuclease and CRISPR technology to establish knock-in, knock-out mice and cell line, genotyping, xenograft, histology techniques. Glycolipid: Ganglioside extraction, Thin layer chromatography (TLC), Dialysis, Affinity chromatography

Bioinformatics: TCGA database, RNA-seq and microarray data analysis using R and Bioconductor Computer skills: Office: Excel, Powerpoint, Word

Database: SQLite

Statistic: R, SAS, MATLAB, GraphPad Prism

Programming language: C++, C, Java, assembly language Web development: HTML, CSS,

Others: Photoshop

Reference

Yanyun Wu, M.D.

Chief Medical Officer

Bloodworks Northwest

921 Terry Ave, Seattle WA

Main line: 206-***-****

Direct line: 206-***-****