Associate Manufacturing Technician

Shayne Strange (They/Them Pronouns)

510-***-**** *************@*****.*** LinkedIn:

Education

Laney College: Oakland, CA- June 2023

Associates Achieved in: Natural Science, Social Science, Humanities, Language Arts Units Completed at Laney College

Certifications

Certificate of Achievement: Biomanufacturing

Skills

Emaint, JIRA, LIMS, cGMP proficient, LCMS-Mass Spectronomy, Cleavage & Deprotection, Chromatography Color Health Genomics/ Clinical Lab Associate October 2021- January 2023

● Aided in routine sample processing in a high complexity CLIA-certified laboratory

● Performed specimen receipt, accessioning, and sample transferring using laboratory automation

● Aided licensed personnel, under direct supervision with routine clinical testing, equipment & reagent validation, and other laboratory activities

● Documented all corrective actions taken when there is a deviation to the test system

● Diagnostic laboratory exposure with high volume automated specimen handling systems, as part of the qualitative and quantitative analysis of human specimens

● Embraced change by keeping an open mind to changing plans and incorporated change instructions into own area of work

● Performed and document routine maintenance of equipment Bio-Techne, ProteinSimple/ Manufacturing Technician Compounding July 2021- October 2021

● Compounded all ProteinSimple reagents to be used in conjunction with ProteinSimple products and tested of products, buffers, separation matrix, protein ladders, and protein markers

● Skilled in using laboratory equipment, centrifuge, micro/serological/multichannel/manual/electronic pipette, water bath, bioreactor, spectrometer, nanodrop, and compressed gas vaults

● Use of GMP while compounding reagents, filling out detailed bath records, following SOP for each bulk made

● Excellent time management when multiple bulks were made in one shift to prioritize time sensitive work orders

● Utilized in-glove box protocols

● Proficient in the use of BCA kits

ThermoFisher Scientific/ Associate Manufacturing Technician September 2020-July 2021

● Strict adherence to current good manufacturing practices, standard operating procedures, and manufacturing documentation

● Experienced with the operation of filling, labeling and kitting multiple components of ThermoFisher PCR and Taq COVID 19 testing kits using Hamilton Star

● Clean room experience, while pipetting and filling multiple assays for the PCR kits

● Comfortable with operating and troubleshooting Hamilton filling machines used to fill phage

● Experience with BioMicroLab XL100 labeling machines used to apply a label to filled phage tubes

● Processed work orders for synthesis of oligos, starting with c1 synthesis machines, cleavage and deprotection, reconstitution of the dried oligos, quantitation of the product, using LCMS to test purity and sub-pooling for QC

● Running weekly path checks for the Hamilton machines, as well as daily maintenance to ensure everything ran properly

● Good documentation and file retrieval skills when moving from system to system Laney City College/ Lab Student January 2019-June 2020

● Operations and light maintenance of various equipment: Pipettes, water bath, autoclave, pH meter, balances,centrifuges, and filtration setting with the intent to ready myself for a career in biomanufacturing.

● Biochemical tests used for bacterial identification: TSI, MRVP, indole, citrate, urea, gram staining

● Prepared sterilized media, buffers, and glassware for all personal biomanufacturing lab projects

● Producing solutions of differing mass/vol, percent, molarity and dilute concentrations

● Streaking for isolation on general media, sub-culturing, pouring plates, serial dilution, and antibiotic testing

● Detection and purification of proteins and nucleic acids using a variety of methods: Benedict’s, Iodine indicator, DNA extraction buffers, PstI and EcoRI, electrophoresis, and DNA staining

● Working knowledge: plasmid miniprep, assays for DNA purity & transformation Psoas Massage & Bodywork/ Office Admin/Inventory Manager February 2019-June 2020

● Demonstrated competent time management daily by answering phones, responding to emails and interacting with clients to maintain a 95% daily booking rate for a 7- room clinic that serviced approximately 50 clients and patients per day

● Restructured inventory protocols upon promotion to inventory manager after working 3 months with the company

● Duties involved organization, counting and ordering 23 retail items as well as 80+ necessities needed to maintain clinic functioning week by week

● First point of contact for new and existing clients and patients by performing 15-20 minute intake communication

● Exhibited good teamwork communication, emotional intelligence and leadership by mentoring & training 6 new hires, working with 7 fellow front desk workers, 15 therapists and a chiropractor

● Compiled a daily written document of notable issues/to do items for the following day so the opening/closing shifts had full comprehension of what tasks needed to be focused on Additional Skills

● Elementary French & ASL

● Broad tech proficiency, including skills in iWork, Google Drive, Microsoft Office, Photoshop, Premiere, Millennium POS, and social media

References

Upon Request

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