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Western Blot, SDS PAGE, Cell culture, HPLC, Spin column, MALDI

Location:
Boston, MA
Posted:
March 08, 2023

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Resume:

LISHEL PINTO

Boston, MA +1-857-***-**** advsba@r.postjobfree.com linkedin.com/in/lishel-pinto EDUCATION

Northeastern University, Boston, MA Expected: Dec 2024 Candidate for Master of Science in Biotechnology

Relevant Coursework: Cell Culture Processes for Biopharmaceutical Production, Molecular Cell Biology for Biotechnology, Protein Chemistry

Ramaiah Institute of Technology, Bengaluru, Karnataka Jul 2022 Bachelor of Engineering in Biotechnology

Relevant Coursework: Cell and Molecular Biology, Biochemistry, Microbiology, Drug Design and Development

TECHNICAL SKILLS

Lab Techniques

Dry lab: AutoDock, Discovery Studio Visualiser, Pymol, Chimera, ChemSketch, ImageJ, Biorender Wet Lab: Agarose gel-electrophoresis, Single emulsion technique, Serial dilution, cell counting, passaging cells Instrumentation: Microplate reader, Photoacoustic imaging (MSOT), homogenizer, western blot apparatus, sonicator, UV/Vis spectrophotometer, Western Blot

Analytical Techniques

Software: Microsoft Excel, Word, PowerPoint

Operating System: Window XP/7/10

RESEARCH EXPERIENCE

Research Assistant, Northeastern University Jan 2023 – Present

• Working in Professor Zhaohui Sunny Zhou’s laboratory: https://www.linkedin.com/in/sunnyzhou/

• Studying protein chemistry for conjugation and effective method of drug delivery

• Gaining protein mass spectrometry training and hands-on-skills Research Trainee, Indian Institute of Science, Bengaluru Oct 2021 – Jul 2022

• Examined and contributed towards animal cell culture techniques including passaging cells and cell lysis, protein estimation by Bradford assay, and Western Blot analysis

• Furthered lab setting as a team and collaborated with other lab members Intern, R. V. College of Engineering, Bengaluru Aug 2020 – Sep 2020

• Completed a project titled, ‘Computational study of phytochemical from Moringa oleifera Lam, for SARS-CoV-2’, using molecular docking and visualization to find a potential phytochemical against SARS-CoV-2, along with dynamic simulation

• Explored various tools and applications to gain insight into the elaborate process of drug discovery ACADEMIC PROJECTS

Development and characterization of Indocyanine green coated nanoparticles for studying release kinetics with photoacoustic imaging, Indian Institute of Science, Bengaluru

• Initiated the project in a collaborative environment as part of the undergraduate thesis

• Studied the characteristics of Indocyanine green encapsulated in a polymer for drug release kinetics with Multispectral Optoacoustic Tomography (MSOT) In silico Analysis and Screening of Natural Compounds Against Fluoroquinolone Resistant Mycobacterium tuberculosis, Ramaiah Institute of Technology, Bengaluru

• Organized and supported the team to understand the resistance of Mycobacterium tuberculosis

• Researched and completed the computational preparation of mutant proteins and analysis of drug resistance by in silico molecular docking of mutants and wild type protein

• Performed virtual screening of natural compound database to find potential drug against resistant Mycobacterium tuberculosis

WORK EXPERIENCE

Mediator, Contagion-Science Gallery Bengaluru, Bengaluru Apr 2021 – Jun 2021

• Responded to thought-provoking questions to initiate and facilitate conversations within and beyond program

• Participated in team-based learning with other mediators

• Interacted with artists, scholars, and experts from different countries and mediated to everyone including school students and researchers

LEADERSHIP

Managing Editor, Ramaiah Institute of Technology, Bengaluru Oct 2021 – July 2022

• Co-led and managed a team to release news letters in the department of Biotechnology

• Worked on an interface of science and communication and had the first newsletter released Mar 2021 – Aug 2021

Oct 2021 – Jul 2022

GPA: 3.778

GPA: 8.74



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