Curriculum Vitae

Tao Gao, Ph.D.

**** ******** ****, ****** ****, NJ 08003

Cell phone number: 716-***-****

Email: advg5l@r.postjobfree.com

Immigration status: US Permanent Resident

EDUCATION

Ph.D. in Biochemistry State University of New York at Buffalo, Buffalo, NY (2006)

W ORKING EXPERIENCE

Research Scientist (Genesis Biotechnology) (2021-2022)

Research Associate (Children’s Hospital of Philadelphia) (2014 – 2021) • Retinoic acid signaling in early development of peripheral nervous system.

•RB1 signaling in regulation of development of pericytes

Postdoctoral Fellow and Research Associate (University of Pennsylvania) (2007-2013)

•PDX1-dependent regulation of pancreatic beta-cell reprogramming and diabetes

•Hippo signaling-mediated regulation of pancreas and liver development

•Regulatory roles of PDX1 in progression of pancreatic ductal adenocarcinoma

Research Assistant (State University of New York at Buffalo, Buffalo, NY) (1999 – 2007)

•Iron- and heme-dependent regulation of cytochrome and heme apo-protein folding and stability

R ESEARCH EXPERTISE:

•Drug screening: Small molecule drug design and screening

•Animal disease models: creation and characterization of rodent disease models, including diabetes; pancreatitis; pancreatic ductal adenocarcinoma; liver cancer; early nutrient-induced obesity; Hirschsprung’s disease; intestinal pseudo obstruction

•Transgenic animal techniques: creation of transgenic; knockout; and knockin mice.

•Small animal maintenance, handling,and surgery: maintenance of rat and mouse colonies; writing IACAC protocols; IP, IM, IV injections; oral gavaging; intra-cardiac perfusion; extraction and maintenance of rat blastocysts; rat intra-uterus embryo transfer

•Ex vivo model of early embryonic development: intra-amniotic micro-injection of early mouse embryos; ex vivo culturing of early mouse embryos

•Tissue clearing for whole mount microscopy: Clarity-based clearing of mouse brains for light sheet microscopy imaging; 3DISCO-based clearing of mouse embryos for confocal microscopy imaging

•Pancreatic islet biology: islet isolation; islet perifusion secretion assay; ELISA

•Cell biology: culturing and analysis of embryonic stem cells; flow-cytometry; generation and maintenance of pancreatic adenocarcinoma cell lines; cell-based bioassays; 3D cell culture

•Microbiology and bacteriology: creation of bacterial genomic mutagenesis by conjugation

•Molecular Biology: generation of lentivirus and retrovirus; RNA SEQ; Single Cell SEQ; RT-PCR; bisulfate-sequencing; BAC (bacterial artificial chromosome) recombineering; site-directed mutagenesis; CRISPR gene editing

•Biochemistry: CHIP; protein engineering, expression and purification; SDS-PAGE/western blotting

•Bioinformatics: R language; Ingenuity pathway analysis; Python

SELECTED PRESENTATION AND PUBLICATIONS

•Gao T, Huang K, and Heuckeroth RO. “RB1 signaling is critical for development of pericytes in mice

”. (Manuscript in preparation)

•Gao T, Wright-Jin EC, Sengupta R, Anderson JB, Mendelsohn CL, and Heuckeroth RO.

“Cell-autonomous retinoic acid receptor signaling alters enteric nervous system development with stage-specific effects in mice” JCI Insight 2021;6(10):e145854. https://doi.org/10.1172/jci.insight.145854.

•Wright CM, Schneider S, Smith-Edwards KM, Mafra F, Leembruggen AJL, Gonzalez MV, Kothakapa

DR, Anderson JB, Maguire BA, Gao T, Missall TA, Howard MJ, Bornstein JC, Davis BM, Heuckeroth

RO.”scRNA-Seq Reveals New Enteric Nervous System Roles for GDNF, NRTN, and TBX3” Cell Mol Gastroenterol Hepatol (2021) Jan 11;S2352-345X(20)30213-7

•Gao T “Temporal-Spatial Regulation of Enteric Neuron Development by Retinoic Acid Signaling“ Oral presentation (2018) ENS 5th international symposium at Boston

•Gao T, Mckenna B, Li C, Reichert M, Nguyen J, Singh T, Yang C, Pannikar A, Doliba N, Zhang T, Stoffers D, Edlund H, Matschinsky F, Stein R, and Stanger BZ. “Pdx1 maintains Β-cell identity and function by repressing an α-cell identity” Cell Metabolism (2014) Feb 4,19(2):259-71

•Gao T, Zhou D,Yang C, Singh T, Penzo-Méndez A, Maddipati R, Tzatsos A, Bardeesy N, Avruch J, and Stanger BZ “Hippo Signaling Regulates Differentiation and Maintenance in the Exocrine Pancreas” Gastroenterology (2013) 144(7):1543-1553 Notes: COVER STORY

•Srinivasan M, Dodds C, Ghanim H, Gao T, Ross PJ, Brown RW, Dandona P, Patel MS.

“Maternal obesity and fetal programming: effects of a high-carbohydrate nutritional modification in the immediate postnatal life of female rats.” Am J Physiol Endocrinol Metab

(2008) 295(4):E895-903.

• Gao T, O'Brian MR. “Control of DegP-dependent degradation of c-type cytochromes by heme and the cytochrome c maturation system in Escherichia coli.” J Bacteriol. 2007 Sep;189(17):6253-9

• Gao T, O'Brian MR. “Iron-dependent cytochrome c1 expression is mediated by the status of heme in Bradyrhizobium japonicum.” J Bacteriol. 2005 Aug;187(15):5084-9.

HONORS

•McArthur NIH Travel Award for Best Oral Presentation of Research (short talk) of “Temporal-Spatial Regulation of Enteric Neuron Development by Retinoic Acid Signaling“ at the ENS 5th international symposium at Boston, 2018

•Best Oral Presentation at Biochemistry Symposium at SUNY at Buffalo, 2003

Hobbies

I love playing soccer and am a very good amateur violinist.

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