PRAFULLITA SINGH

Female, ** +91-735******* adt1o2@r.postjobfree.com

EDUCATION

M.Tech-Biomedical Engineering 2019 Gautam Buddha University,Greater Noida 7.8/10 B.Tech - Biotechnology 2017 Allahabad Agriculture University, U.P. 8.2/10.0 CBSE (Class XII) 2013 Kendriya vidyalaya New Cantt Alld(U.P) 65.4% CBSE (Class X) 2011 Kendriya Vidyalaya New Cantt Alld, (U.P) 7.8/10 PROFESSIONAL EXPERIENCE

GBU, Greater Noida Biotechnology Department (Jan. – June. 2019)

• Functional and biochemical analysis of Pseudomonas aeruginosa strain 27853 using insilico.

• Cloning, Expression and Purification of PA-1(Toxin) from Pseudomonas aeruginosa. DRDO, Delhi Intern INMAS Department ( Jan. – June 2017)

• Worked as intern on project entitled “Therapeutic role of SKMB-90 in topical wound healing and tissue repair” It is a record of work, I carried under the supervision of Dr. Mitra Basu, Scientist “F”, INMAS, DRDO Delhi.

• Technical Proficiency in animal handling, Toxicity evaluation, Histopathology.

• Created a New Instrument to check Tensile Strength of Tissues named ‘TENSOMETER’. Biotech Park, Lucknow Intern Molecular Biology Department (June – July 2016)

• Training on Molecular Biology – “Qualitative and quantitative analysis of DNA from human blood, chilli Plant and E.coli and estimation of plant protein by Lowry’s method” under the supervision of Senior Scientist Dr Uma Shankar. ICAR-Indian Institute of Sugarcane Research Intern Agriculture Department (June – July 2015)

• Training on – “Application of Tissue Culture Technique in Sugarcane Improvement” under supervision of Senior Scientist Dr. R.K.Singh.

MRD Life Sciences Intern (Dec. – Jan. 2015)

• Rigorous Training on “Advanced Biotechnology Techniques and Instrumentation”. CONFERENCES AND WORKSHOPS

• Attended and actively participated in International Conference on “Future Prospects of Advancement in Biological Sciences, Health Issues and Environment Protection” at Lucknow.

• Actively participated during Ciencia Fusion – on the occasion of National Science Day on the theme of ‘Fostering Scientific Temper’.

ACADEMIC ACHIEVEMENTS AND AWARDS

• Secured first position in Collage Competition at Ciencia Fusion, SHUATS. POSITION OF RESPONSIBILITY

• Core member of the Society of Microbiology, SHUATS.

• Coordinator in Techno-Cultural Fest Involution, SHUATS.

• Volunteer in one of the biggest function of college, 61st Anand Utsav 2015, SHUATS. TECHNICAL PROFICIENCY

Laboratory Expertise

• Cloning, protein purification and expression, Toxicity Assay

• Sterilization Technique - Autoclaving, working in Laminar Air Hood and Radiations.

• Microbial Technique - Media preparation, plating, culturing & sub culturing of microbes.

• Plant Tissue Culture - Embryo culture and callus culture.

• Molecular techniques - Agarose Gel Electrophoresis, SDS-PAGE, Genome Isolation, PCR, Nanodrop Technique, Enzyme Assay, Southern Blotting.

• Chromatography - Paper Chromatography, NI-NTA

• Instrumentation - Microscopy, Centrifugation, Spectroscopy. Bioinformatics implements and Technical efficiency :

• Biological Database, Structural Visualization and prediction.

• 3-D structure Modeling, Validation of structure

• Operating system: Windows, Proficient use of MS Office. Interpersonal Skills :

• Communication Skills – Host in various seminars, cultural and non-cultural events.

• Experience of working in Lab with team as well as individually.

• Flexible to learn and adapt new technologies.

• Optimist, self-confident and determined.

Technical Skills – C++ Hobbies – Badminton, Cooking and Travelling.