Research Fellow Associate
Resume:
DONGMEI HU
Abcam
Milpitas, CA *****
*******@*******.***
QUALIFICATIONS
Antibody
Ten years work on Antibody purification and characterization. Well trained in Western Blotting, ELISA, IF and Flow Cytometers. Experience in immunological assays such as immunoprecipitation and immuno-absorption. Experience in related products conjugation and labeling.
Protein Sciences
Well trained in the protein science including protein over expression, purification, and characterization. Experienced in biochemistry and biophysics with the techniques such as electrophoresis, HPLC/FPLC, centrifuging.
Molecular and Cell Biology
Experiences include primer design, cloning, PCR, transformation/transfection, and cell cultures.
Computer Skills and Others
Ability to use computer programs, including Microsoft Office (Word, Excel), and some graphic/analytical programs such as SigmaPlot. Excellent organizational skills in the performance of job duties.
EXPERIENCE AND SKILLS
ABCAM 2021-Present
Senior Associate Research (R&D Support Production)
Protein purification and enzyme active test:
Kit Troubleshooting: ELISA kit and Enzyme activity assay kit
Endotoxin Free Production, QC
BIOVSION 2012-2021
Associate Research III (R&D, Protein)
Antibody purification and characterization. Western Blotting, ELISA, IF and Flow Cytometers.
Cell culture: Hela, 3T3 and T cell.
Kit development and Troubleshooting: such as KLH and BSA Conjugation Kit, Antibody purification Kit, Glycoprotein Stain Kit et al.
Endotoxin Free Product development, production and QC
PARKINSON’S INSTITUE AND CLINIC, SUNNYVILE 2009-2012
Associate Specialist
LRRK2 is a large, widely expressed, multi-domain and multifunctional protein. LRRK2 mutations are the major cause to inherited and sporadic PD. I worked on mutant of LRRK II and some related protein, using techniques of plasmid purification, transformation, PCR.
Working on the compounds screening and characterization target to the Parkinson’s disease. The studies involve in vitro experiments using an alpha-synuclein fibrillation model, and in vivo studies in the monkey model. Biochemistry and clinical skills are used in these studies.
UNIVERSITY OF CALIFORNIA, SANTA CRUZ 2002-2007
Associate Specialist
Screening of pharmaceutical compounds to Parkinson’s disease. Looking for the inhibitors of -synuclein fibrillation for development of drugs against Parkinson’s disease. Techniques include fluorescence spectrum, mass spectrum, HPLC, electrophoresis, electronic microscopy, and mammalian cell cultures.
Protein mutagenesis: comparing kinetics of -synuclein mutant fibrillation and image of -synuclein wild type and mutant fibrils, using techniques of plasmid purification, transformation, PCR.
Conduct research for the mechanism to amyloidosis (Antibody light chain deposit diseases) and Parkinson’s disease and screening of their inhibitors. Proteins (SMA and LEN) over expression in E. coli and purification using conventional biochemistry methods (liquid chromatography, electrophoresis, extra speed centrifuge, HPLC/FPLC, et al.). Protein structures analysis using various biophysical methods.
KANSAI MEDICAL UNIVERSITY, JAPAN 2000–2001 Research fellow
Kinetics of protein folding at subzero temperature. Refolding of -lactoglobulin, ubiquitin (F45W) and other proteins/peptides at subzero temperature. Two mutants of -lactoglobulin were separated using ion-exchange chromatography. Folding kinetics as well as protein structures were studied using a stopped-flow apparatus coupled to far-UV CD, intrinsic fluorescence and SAXS (small angle x-ray scattering).
EDUCATION
SHANDONG UNIVERSITY,MEDICAL SCHOOL, CHINA
Bachelor of Medicine
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