Biomedical Engineer Lab Assistant

Alex Paul

*********@*****.***

630-***-****(cell)

Education

Cornell University

Graduate May 2021: Master’s in Biomedical Engineering

Loyola University Chicago

Graduated May 2017: Bachelor’s in Molecular Biology Major, English Minor

Professional Experience

Dr. Doering’s Genetics Lab Chicago, IL

Lab Assistant January 2014-May 2017

Handled DNA with appropriate lab techniques and recorded results.

Grew cell cultures in test tubes and agarose plates.

Made molecular assays to analyze DNA

Created multiple assays and buffers to run experiments

Analyzed DNA with Geneious Version 7.1.5 program and Quantitative Tool for Methylation Analysis (QUMA) program to determine methylation patterns on DNA for varying cancer cell lines.

Designed own primers for PCR protocol

Wrote thesis and presented on project on White Blood Cell, Placenta, and Hybrid cancer cell lines

Dr. Reilly’s Psychology Lab Chicago, IL

Lab Volunteer September 7th, 2018 – November 2018

Worked with lab rats, assisted with cleaning cages and running drinking protocols

Injected rats with lethal dose of formaldehyde in order to perform dissections

Perfused blood out of body in order to properly remove brain

Cut sections of brain and stained to see which neurons were not active, which may have affected behavior

Litholink Chicago, IL

Medical Technologist March 2019- May 2020

In charge of quality control of patient urine samples and analysis of oxalate, citrate, potassium, sodium, pH levels, fluoride, and other chemistries.

Overlooked reports to ensure levels were consistent and as expected to prevent error.

Utilized online database to track various patient samples and manage actions on each of them.

Worked in a fast-paced, team environment to meet deadlines

Dr. Lammerding’s Research Lab Ithaca, New York Biomedical Engineer, Researcher August 2020- May 2021

Collaborated with a team to create efficient designs for uniaxial cell stretcher, PDMS well to hold cells, and microscope software to record live cell imaging.

Grew and ran strain experiments on HeLa and mouse dermal fibroblast cell cultures

Utilized CAD software such as Fusion 360 and Matlab to design stretcher and computer software.

Experience with 3D printing and CNC to design necessary parts for project.

Created a pitch video of product, project presentation, and formal report.

Vertex Pharmaceuticals Cambridge, Massachusetts Process Development Senior Research Associate July 2021- Present

Culture and passage embryonic stem cells for gene and cell therapy on 2D scale using well plates, T225 flasks, and cellstacks.

Adapt cells onto a 3D scale using Das Box, BioBlue STRs, and PBS 3L bioreactors.

Scale up cell cultures with Pall and Sartorius 50L bioreactors.

Differentiate embryonic stem cells into beta islets by formulating growth factors.

Measure and observe daily cell growth using Nova Prime Analyzer and Nikon clinical compound microscopes

Creating insertable device to patients to cure type one diabetes.

Skills: Detail oriented, proficient in word, excel, powerpoint. fluent in english, excellent communicator, experience with genetic softwares and databases (Geneious and QUMA),excellent in following lab protocols, pipetting, working with lab glassware, comfortable when working with live and dead rats, dissecting rats and extracting organs, observing animals for changes in behavior or physical condition, carefully handling toxic, hazardous and sharp materials, recording and presenting data, explaining lab findings, CAD software such as Fusion360 and Matlab, Python and bioinformatics for molecular analysis, Protein Analysis using Chimera, writing research grants and paper works.

Lab Equipment Competencies: Polymerase Chain Reaction, Gel Electrophoresis, Cell Culturing, DNA Staining, pH meter, Pipette, Bunsen Burner, UV Transilluminator, EMSA, Southern Blotting, Centrifuging, Vortexing, Liquid chromatography (HPLC), Cell Transformation, Infrared Spectroscopy, Titration, Microscope, ManSci, Distillation Apparatus, Rat Dissection, Tecan, and Beckman machines, NextGen Sequencing, Flow Cytometry, T225 flasks, Cell Stacks, 3L Bioblue STRs bioreactor, 200 mL Das Box bioreactor, 3L PBS bioreactor, 50L Pall bioreactor, 50L Sartorius bioreactor.

Areas of Knowledge and Research Interests: Molecular Genetics, Biochemistry, Genomics, Biomolecular Engineering, Microbiome Engineering, Protein Structure and Function, Bioprocess Engineering, Tissue Engineering, Stem Cell Engineering, Regenerative Medicine, Synthetic Biology, Molecular Mechanisms of Disease, Computational Biology, Pharmacology.

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