Dr Thammineni Krishna Latha, Ph.D.

Tutor, Department of Biochemistry, Government Institute of Medical Sciences (GIMS), Greater Noida, UP 201310.

Email Id: adruu4@r.postjobfree.com, Mobile No. +91-920******* Work Experience

Nov 2019-Present: Government Institute of Medical Sciences (GIMS), Greater Noida, UP.

Role: Tutor in Dept. of Biochemistry

Summary of Experience:

Teaching Biochemistry to First year MBBS students, conducting Practicals, tutorials, and SDL.

Worked in COVID Molecular Lab during lockdown for the diagnosis and confirmation of COVID by RT-PCR.

2014-2019: UCMS & GTB Hospital, University of Delhi, Delhi. Assisted MD students for their thesis work in Molecular techniques like DNA Isolation, RNA Isolation, PCR, Real time PCR, DNA Polymorphism, micro–RNA Isolation and Expression, Organochlorine pesticides Isolation and Quantification etc, Supported PhD Supervisor in reviewing various PhD theses, manuscripts, reviews and for preparing question papers for first professional MBBS exams etc. EDUCATION

2014-2021 Ph.D. in Medical Biochemistry (Thesis Submitted in Jan 2021 and Awarded in May 2022)

UCMS and GTB Hospital, (Faculty of Medical Sciences, University of Delhi), Delhi.

Thesis Title: “A study to assess an association of Organochlorine pesticide exposure with hypoxia and lymph node metastasis in the patients of breast cancer”

2008-2011 Master of Science in Medical Biochemistry (66%) JIPMER, Pondicherry, India.

2004-2007 Bachelor of Science in Biotechnology (74.5%) Sri Krishnadevaraya University, Kurnool, AP, India. 2002-2004 Board of Intermediate education (86.8%)

Rao’s Junior College, Nandyal, Andhra Pradesh, India. 2001–2002 Board of Secondary school education (85.4%) Nehru high school, Banagana Palle, Andhra Pradesh, India. Research Experience

2014-2021 UCMS and GTB Hospital, (University of Delhi), Delhi Thesis Title: “A study to assess an association of Organochlorine pesticide exposure with hypoxia and lymph node metastasis in the patients of breast cancer”

The incidence of breast cancer in India is on rise, superseding the cervical cancer in urban India. The etiology of breast cancer is multifaceted, involving genetic, epigenetic, lifestyle and environmental factors, however, the true burden of environmentally induced breast cancer has been grossly underestimated. The current study has recruited 100 breast cancer patients as cases and 100 benign breast disease patients as controls. Quantification of OCPs was done by Gas Chromatography. Analysis of hypoxia and metastasis promoting genes was done at mRNA and protein level by Real time PCR and Western blot, respectively.

2009- 2011 Department of Biochemistry, JIPMER, Puducherry, India. Title of the project: Assessment of different biomarkers in the early prediction of Tubal Ectopic Pregnancy

LABORATORY SKILLS:

Isolation of DNA from various sources (Bacterial genomic, Plasmid, Plant, Animal & Blood), Isolation of RNA and Protein, Isolation of micro RNA and analysis of miRNA expression, Restriction Analysis of DNA, Extraction of Organochlorine Pesticides (OCPs) from the tissue and estimation by Gas Chromatography, Conventional PCR and Real time PCR, Western blotting, Electrophoretic techniques (Agarose and SDS PAGE), DNA Polymorphisms, Transformation, ELISA, Enzyme assays [Estimation of Km & Specific activity of enzymes, Estimation of total activity of enzymes such as ALP, ACP, ALT, AST, Urease & Amylase], LFT, KFT, Chromatography techniques (Paper, Thin layer & Ion Exchange chromatography).

Publications:

1) Thammineni KL, Thakur GK, Kaur N, Banerjee BD. Significance of MMP-9 and VEGF-C expression in North Indian women with breast cancer diagnosis. Molecular and Cellular Biochemistry. 2019 Jul 15;457(1-2):93-103. 2) Latha TK, Verma A, Thakur GK, Banerjee B, Kaur N, Singh UR, Sharma S. Down Regulation of KAI1/CD82 in Lymph Node Positive and Advanced T-Stage Group in Breast Cancer Patients. Asian Pacific journal of cancer prevention: APJCP. 2019;20(11):3321.

3) Thakur GK, Sharma T, Latha TK, Banerjee BD, Shah HK, Guleria K. High Resolution Based Quantitative Determination of Methylation Status of CDH1 and VIM Gene in Epithelial Ovarian Cancer. Asian Pacific journal of cancer prevention: APJCP. 2019;20(10):2923.

4) Banerjee BD, Kumar R, Thamineni KL, Shah H, Thakur GK, Sharma T. Effect of Environmental Exposure and Pharmacogenomics on Drug Metabolism. Current Drug Metabolism. 2019 Dec 1;20(14):1103-13.

5) Kaur N, Swain SK, Banerjee BD, Sharma T, Krishnalata T. Organochlorine pesticide exposure as a risk factor for breast cancer in young Indian women: A case–control study. South Asian Journal of Cancer. 2019 Oct;8(4):212. 6) Rai S, Garg PK, Bhatt S, Latha TK, Verma AK, Banerjee BD, Singh MP. The diagnostic role of microRNA 21 in patients with nonsmall cell lung cancer: An exploratory study. Lung India. 2020 Nov 1;37(6):501. 7) Garg PK, Chishi N, Kumar R, Latha TK, Rai S, Banerjee BD, Gupta S. TISSUE LEVELS OF ORGANOCHLORINE PESTICIDES IN BENIGN AND

MALIGNANT BREAST DISEASE: A COMPARATIVE EXPLORATORY STUDY. J Environ Pathol Toxicol Oncol. 2021;40(1):43-50. 8) Soundravally R, Krishna Latha T, Soundara Raghavan S, Ananthanarayanan PH, Srilatha K. Diagnostic significance of total creatine kinase and its isoform in tubal ectopic pregnancy. Journal of Obstetrics and Gynaecology Research. 2013 Dec;39(12):1587-91.

Posters and seminars presented in the conference.

1. Presented a poster entitled “Possible role of Organochlorine Pesticides as a etiological factor in Breast cancer” at ACBICON 2021 Organized by the Department of Biochemistry, College of Medicine & JNM Hospital, WBUHS, Kalyani, Nadia 741235, under aegis of The West Bengal University of Health Sciences, Dec 2021.

2. Presented a poster entitled “Combined evaluation of VEGF-C and MMP9 Expression in relation to lymph node metastasis in Breast cancer” at 7 th

International conference on translational cancer research at IIT-Chennai, Feb 8-11, 2018.

3. Presented a poster entitled “A study to assess an association of Organochlorine Pesticides with the risk of Breast cancer” at “International Conference on Potential Impact of Pesticides on Environment and Human Health” (ICPIPEHH- 2017) at Dayananda Sagar University, Bangalore during 2 nd

– 4

th

November, 2017.

4. Attended & Presented a review poster in an International Conference on Environmental Mutagenesis, Carcinogenesis and Health and 40th Annual Meeting of Environmental Mutagen Society of India (EMSI) from February 17-19, 2016

(ICEMCH-2016).

5. Presented a seminar on “Pesticides-Double edged sword” in Bio-Zest 2018 conducted by South Asian University, Delhi, held on 6 th

December 2018.

Attended Workshops and Conferences

Symposium on “Cellular & Molecular Biomarkers” conducted on 14th May 2022 by Department of Biochemistry, AIIMS, Rajkot.

Attended North Zone ACBICON 2022 on “Biochemical and Molecular basis of Lifestyle diseases” conducted by PGIMER Chandigarh from April 22 nd

-23

rd

2022.

ACBICON 2021 held hybrid mode from 12

th

to 15

th

December 2021.

CME on “COVID-19 Preparedness and Response Through the Lens of Health Systems” organized by department of Medical Education, GIMS, Greater Noida on 30

th

, Oct. 2021.

CME cum Workshop on “Recent advances in CSSD and its role in Hospital infection control” organized by department of Microbiology, GIMS, Greater Noida held on 14

th

September 2021 at GIMS, Greater Noida.

Webinar on CRISPR by Department of Biochemistry, St Joseph College, Kurnool AP on 16

th

July 2021.

Attended a workshop conducted by DBT on “Basic techniques in Genomics” at Shillong, held from the period of 17

th

Nov- 21

st

Nov, 2014.

Attended a 7

th

Science conclave conference at IIIT, Allahabad, held from the period of 8

th

Dec-12

th

Dec, 2014.

Attended the workshop on "basic molecular biology techniques in cancer research" at ICPO (Institute of Cytology and Preventive Oncology), Noida held in the period of 4-7 April, 2016.

Attended a workshop on HPLC at Schimadzu Analytical Pvt Ltd, Delhi held from 7

th

-8

th

May 2014.

Attended a workshop on FACS, at UCMS, Delhi.

ACCOMPLISHMENTS:

Completed “Baic Course in Biomedical Research” conducted by ICMR-NII with 78% in Proctored Examination.

Got Best Poster award (NIMS Award) in ACBICON 2021.

Received Certificate of appreciation for contributing in COVID care in Molecular and Diagnostic and Research Laboratory, GIMS.

Qualified the All India CSIR-NET-JRF (Junior Researh Fellowship) Life sciences Examinaion.

Qualified the All India CSIR-NET-LS (Lectureship) Life sciences Examinaion.

Secured the mentioned ranks in National wide Entrances conducted for master’s degree.

Rank 1, JIPMER (Medical Biochemistry), Pondicherry, India.

Rank 3, Banaras Hindu University (Biochemistry), Varanasi, India

Rank 43, Pondicherry Central University (Biochemistry). Pondicherry, India

Rank 5, Sri Krishna Devaraya University (Biotechnology), Ananthpur, Andhra Pradesh.

Rank 9, Sri Venkateshwara University (Biochemistry), Tirupati, Andhra Pradesh. REFERENCES

Prof B D Banerjee

Department of Biochemistry,

UCMS and GTB Hospital

Dilshad Graden

Delhi-110095.

Phone: 882-***-****

Email:

adruu4@r.postjobfree.com

Prof. Zachariah Bobby

Head of the Department,

Department of Biochemistry,

JIPMER, Puducherry, 605006.

Phone: 944-***-****

Email: adruu4@r.postjobfree.com

Brig. Dr Rakesh Gupta

Director,

GIMS,Greater Noida,

UP-201310

Phone: 985*******

E Mail: adruu4@r.postjobfree.com

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