Professor Molecular

Sina Mirzaahmadi, Ph.D.

Curriculum Vitae

Address:

Department of Genetic, Faculty of Basical Medicine and Science, Zanjan Azad University, Etemadieh Street, Zanjan, Iran P.O. Box: 451**-*****

E-mail: **********@*****.***, **********@*****.***

Phone: +982*-********, +98-912*******

Educational background (date, degree, title, institute):

D.V.M., Tabriz branch, Azad University of Tabriz, IRAN; (mm/dd/yyyy), (09/23/1998 to 01/19/2005).

D.V.M. thesis: The Study of Dexamethasone induced Apoptosis in Hippocampus of Rats.

Ph.D., in Molecular Genetics, Genetic Department, science and research branch, Azad University of Tehran, IRAN; (01/21/2006 to 08/01/2011).

Ph.D. thesis: Molecular cloning, Stable expression and Functional Analysis of Recombinant Human FVII gene in Leishmania Tarentolae Chromosome.

Employment(s): (date, post, place)

Primary Appointment:

1.Assistant Professor, Department of Genetics, Zanjan Branch, Zanjan Azad University, Zanjan, Iran (02/20/2006 to present; 16 years 1 month).

Secondary Appointment:

1.Supervisor of Molecular Unit, Massoud Medical Laboratory, Tehran Province, Tehran, Mirdamad Blvd. Phone: +98(21)85586. From (03/21/2015 to 02/21/2022; 7 years).

Other Responsibility:

1.Head of Salamate (Health) Zanjan, Food and Drug laboratory, Saadi Ave, Zanjan, Iran (02/02/2019 to now; 3 years 1 month).

Key quantification:

As a University Professor:

Worked as a university professor, researcher and thesis manager in the field of a variety of topics including the use of gold nanoparticles or nano probes in the identification of pathogenic microorganisms, the potential usage of microRNAs, LncRNAs as molecular biomarkers in disease diagnosis, cancer biology and cell research and cultivation of different cancer cell lines; Exempt of research, teaching and preparation of main syllabus of some genetic category lessons to learn students promptly, theoretically or experimentally. As a head of department, preparing of educational chart of at least of four different entrance of students in each semester, and confer with department professors to timing and attendance periodically to educate students. Attending in intra faculty periodical conferences with other lecturers to increment student’s scientific knowledge, specifically my lecture topics were about genetics and awareness of human disabilities.

As a Lab Scientist in Masoud Lab:

1.Supervision and optimization of home-made method to the identification of CMV, HSV1, HSV2, HBV, from serum in Masoud lab.

2.Supervision and optimization of home-made method to the identification of Aspergillus spp., Dermtophytes from granular samples in Masoud lab.

3.Supervision of current thrombophilia panel gene mutations in Iranian RSA patients referred to Masoud Lab.

4.Supervision and optimization of homemade method to the identification of HLA-B 27, 5, 51, 8 alleles from whole blood in Masoud lab.

5.Supervision and optimization of solid tumors gene mutations for prognostic or treatment purposes, like K-RAS, N-RAS, BRAF, EGFR,…

As a Head of the Salamate Zanjan Lab:

1.Supervision of general microbial and chemical tests performance in the lab.

Optimization of the

2.The Multiplex PCR to detection of some food bacteria like Bacillus cereus, Listeria monocytogenesis, Shigella flexneri, …

3.Optimization of Real time PCR method for the detection of Alicyclobacillus spp. in fruit juice.

Implementation of Main Tasks as a member of university professor, including:

a.Preparation of teaching material including course outline, syllabus and lesson plans, revising the subject matter on a regular basis as appropriate for that subject, especially for M.Sc. students in the topics of molecular genetics, molecular biomedicine, human molecular genetics, population genetics and immunogenetics.

b.Monitoring students’ performance in their subject areas, Set and mark exams, Courses evaluation, examination and assessments.

c.As a head of groups for several years, preparing students and professors’ educational curriculum, teaching methods and the study of evaluation score of professors in their courses performed by students.

d.Carry out research undertaking consultancies for students in a particular field of knowledge and learning technical skills.

e.Preparing and compilation of curriculum chart of bioinformatics course for students in B.Sc. level adapted from world’s top universities.

f.Supervise and conducting students’ research and undertaking consultancies in practical work in particular molecular genetics fields and management of holding journal clubs to improve scientific level of students and helping them to thesis preparation and participate in evaluation of their thesis.

g.Participate in departmental and faculty seminars aimed at sharing research outcomes based on both theoretical and experimental experiences.

h.Conducting departmental and faculty seminars by helping research chancellor of Zanjan University and student’s genetic science association.

i.Deliver papers at professional association meetings, seminars and conferences.

j.Attending as a speaker and the member of human genetic panel jury in the intra congress by the subject of new advances in cellular and molecular biology.

k.The internal manager and a member of editorial staff of scientific magazine by the subject new advances in cellular and molecular biology.

Teaching Experiences:

Teaching of Some Lessons in Bachelor Level (02/20/2006 to now)

1.Cellular Biology Lesson (both Theory and Laboratory)

2.Basic Genetics Lesson (both Theory and Laboratory)

3.Molecular Genetics Lesson (both Theory and Laboratory)

4.Population Genetics Lesson(Theory)

5.Human Genetics Lesson (Theory)

Teaching of Some Lessons in M.Sc Level (09/22/2012 to now)

1.Immunogenetics theory Lesson (Theory)

2.Human Genetics Lesson (Theory)

3.Population Genetics

Supervisor and Adviser of M.Sc. theses:

1.Early in 2012

1.1- Optimization of gold nano particle based colorimetric assay to determination of Shigella flexneri, Ecoli O157, Listeria monocytogenesis, Bacillus cereus, Brucella melitensis, Helicobacterium pylori.

1.2- Optimization of gold nano probe based colorimetric assay to determination of Shigella flexneri, Listeria monocytogenesis.

2.Early in 2013

2.1- Optimization of Multiplex QF-PCR for Rapid diagnosis of Hereditary Multiple Exostoses Causing Genes.

2.2- Screening of LCT-22018G>A, LCT-13910C>T single nucleotide polymorphisms as a predictor of infant type hypolactasia in Tehran-Iran.

3.Early in 2014

3.1- Evaluation of relationship between mtDNA microdeletions and Alzeimer disease in patients with maternal familial history referred to FAM center.

3.2- Evaluation of relationship between mtDNA copy numbers and Alzeimer disease in patients with maternal familial history referred to FAM center.

4. 2015

4.1- Investigation of the expression levels of miR-146b, mir-221 as a tumor marker for early diagnosis of thyroid cancer.

4.2- The study of altered urinary exosomal MicroRNA-145 expression as a biomarker for early detection of nephropathy in patients with diabetes mellitus by RealTime-PCR.

5. 2016

5.1- Screening of HLA-DRB1*0301 and HLA-DRB1*0401 haplotypes of patients with type 1 diabetes mellitus in Zanjan.

5.2- Evaluation of Breast cancer samples for their some characters including, antigen expression detected by IHC and CISH, drug resistance genes expression detected by Real-Time PCR.

5.3- Evaluation of relationship between microRNA machinery mutations and .recurrent spontaneous abortion.

6. 2017

6.1- Investigation of the proportional frequency of the chromosome Y bearing sperms transferring fromAlbumin column in comparison with X bearing ones by Real time PCR method. 6.2- Optimization of MLPA method to simultaneous detection of thrombophilia induced gene mutations.

6.3-Investigation of H19, BGL3 lncRNAs expression alterations as a biosensor to alteration of bcr- abl transcript level in patients with positive bcr-abl fusion transcript in response to Anti tumor therapy.

7. 2018

7.1-Evaluation of the effect of TMZ drug in expression alternation of LncRNA Thrill controlling TNF-a pathway in Glioblastona cell line of brain tumors.

7.2- Investigation of gene expression alternation of lncRNAsmad7and lncRNAGAS5 as inhibitor and inducers of apoptotic pathways in MCF7 breast cancer cell line under treatment with methotrexate and cyclophosamide chemotherapy drugs.

7.3- Investigation of relationship between HLA alleles (some HLA-B sub groups) with some disorders like spondyloarthritis, Behcet syndrome.

7.4- Evaluation of the effect of Temodar drug in expression alternation of LNCRNA LINP1controlling NHEJ pathway in Glioblastona cell line of brain tumors

7.5- Investigation the relationship between LncRNA NKILA controlling NF-KB signaling pathway in glioblastoma cell line of brain tumors under treatment by temozolomid chemotherapy drug. 7.6- Analysis of STAT3 and AP1 genes expression alternations under regulation of NF-κB signaling pathway in MCF-7 breast adenocarcinoma cell line under treatment with Cyclophosphamid and Methotrexate chemotherapy drugs.

7.7- Evaluation of UCP2 expression by real time PCR and sequence alterations of UCP2 in Iranian breast cancer patients.

7.7- Analysis of the correlation between Linc-RNA-21, controlling Wnt signaling pathway expression, in Glioblastoma cell line of brain tumors under treatment by Temozolomide (TMZ) chemotherapy drug.

8.2019

8.1-Evaluation of the effect of thiosemi carbosan complex in CASC2 lncRNA expression alternation, regulating mir34 and mir200 and the probability of its effect in Notch signaling pathway in Jurkat E6.1 Acute Lympholastic Leukemia cell line.

8.2- Analysis of thiosemi carbazon complexes effects on LUNAR1, NALT1 lncRNAs and Notch1 target, gene expression alternations, regulating Notch signaling pathway in Jurkat E6.1 Acute Lympholastic Leukemia cell line.

8.3- Evaluation of the effect of thiosemi carbazon complex in CASC2 lncRNA expression alternation, regulating mir34 and mir200 and the probability of its effect in Notch signaling pathway in Jurkat E6.1 Acute Lympholastic Leukemia cell line.

8.4- Evaluation of CRNDE lncRNA gene expression alternation in T98G glioblastoma cell line under treatment by TMZ chemotherapy drug and effect comparison with thiosemicarbazone complexes of Ni and Cu.

8.5- Evaluation of gene expression alternations of NEAT1 and GAS5 lncRNAs in T98G glioblastoma brain tumor cell line under treatment by TMZ chemotherapy drug and its comparison with thiosemicarbazone Ni and Cu complexes.

9.2020

9.1- Evaluation of SPATA18, P53, and RFN38 RNAs expression level in sperm samples obtained from asthnospermia and normospermia individuals.

10.2021

Evaluation of gene expression alternation of ANRIL and CCAT1 LncRNAs,… in Jurkat E6.1 cell line acute lymphoblastic leukemia under treatment with chemotherapy drugs and thiosemicarbasone complexes.

11.2022

Evaluation of the effect of thymol composition on the process of kidney carcinogenesis induced by diethylnitrosamine carcinogen and changes of the expression of genes EMT, Wnt and angiogenesis pathways in male mice.

Publications:

1.Relationship between SPATA18 and P53 Gene Expressions in the Sperm Cells Obtained from Normospermic and Asthenospermic Samples, Alireza Panahi, Sina Mirzaahmadi, Golnaz AsaadiTehrani, International Journal of Fertility and Sterility, 2022(under press).

Abstract

Introduction: Improving sperm motility results in increasing the success of a treatment cycle. Recently, sperm RNA can be used for diagnostic purposes such as whole seminal fluid and sperm analysis and sperm quality test in the patients undergoing IVF/ICSI.

2.Evaluation of CAMD1 gene promoter hypermethylation in HPV positive cytological samples of cervical cancer, Jalili F., Asaadi Tehrani G., Mirzaahmadi S, Middle east journal of cancer, 2022 (under veriew).

3.Developing the Expression of Recombinant Human Coagulation Factor VII by the Lizard Leishmania Expression System, Sina Mirzaahmadi, Golnaz Asaadi-Tehrani, Mojgan Bandehpour, Nooshin Davoudi, Leila Tahmasbi, Nahid Hosseinzadeh, Hasan Mirzahoseini, Kazem Parivar, Bahram Kazemi, Highlights on Medicine and Medical Research Vol. 14, 68-79, 2021.

4.Evaluating the clinical application of nested PCR in the diagnosis of fungal infections, Nasrin Sheikhi, Zahra Rafat, Saeed Zaker Boostan Abad, Sina Mirza Ahmadi, Reza Azizian, Masoud Hamidi, New Zealand Journal of Medical Laboratory Science 74 (2), 115-118, 2020.

5.Next Generation Sequencing a Method for Identifying Genetic Mutations Associated with Spina Bifida Disorder, Hanieh Naddaf, Arash Sattari, Sina Mirzaahmadi, Pajouhan Scientific Journal 17 (2), 37-44, 2019.

6.The association between NFKB1-94ATTG ins/del and NFKB1A 826C/T genetic variations and coronary artery disease risk, Abbas Seidi, Sina Mirzaahmadi, Khalil, Mahmoodi, Mohammad Soleiman-Soltanpour, Molecular Biology Research Communications 7 (1), 17, 2018.

7.Relationship between LncRNA THRIL expression controlling TNF-alpha pathway in glioblastoma cell line under temozolomide treatment, Leila Hassani, Golnaz Asaadi Tehrani, Sina Mirzaahmadi, Tehran University Medical Journal TUMS Publications 76 (7), 469-476, 2018.

8.Evaluation of additional Abcc12 gene expression character in breast cancer samples using formal diagnostic profile, Maryam Esmaeili, Sina Mirzaahmadi, Golnaz Asaadi Tehrani, Gene Cell Tissue 5, e84392, 2018, 2.

9.Investigation Of Relationship Between Microrna Machinery Genes Polymorphisms (RAN RS14035, DICER RS3742330) AND Recurrent Spontaneous Abortion In City of TEHRAN, Sohy Amir Fallah, Bostanabad Saeed Zaker, Mirzaahmadi Sina, NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL 8 (29), 23-29, 2018.

10.The study of Relationship between miRNA Machinery Genes polymorphism (DROSHA & EXPORTIN5) and recurrent spontaneous abortion in city of Tehran, Seyed Amin Ashkan, Saeed Zaker Bostanabad, Sina Mirzaahmadi, New Cellular and Molecular Biotechnology Journal 8 (30), 45-52, 2018.

11.Molecular Diagnostics with Laboratory Finding in Patients with Myeloproliferative Neoplasms, Zahra Makani, Sina Mirzaahmadi, Marzieh Motallebi, Abolfazl Movafagh, Iranian Journal of Public Health 46 (11), 1598-1600, 2017.

12.Genotyping and Molecular Characterization of Dermatophytes Isolates Collected from Clinical Samples, Sheikhi Nasrin, Mirzaahmadi Sina, Archives of Pulmonology and Respiratory Care 3 (1), 052-057, 2017.

13.Molecular Diagnostic With Laboratory Finding In Patients With Myeloproliferatove Neoplasms, Golmakani Zahra Bagherzadeh, Sina Mirzaahmadi, Marzieh Motallebi, Abolfazl Movafagh, IRANIAN JOURNAL OF PUBLIC HEALTH 46 (11), 1598-1600, 2017.

14.Comparison and evaluation of laboratory diagnostic method-smear, culture, molecular-in the fungal infection (Aspergillus, Dermatophyte and Mucor), Saeid Zaker Bostanabad, Sina Mirzaahmadi, New Cellular and Molecular Biotechnology Journal 7 (25), 19-26, 2017.

15.The Stature of Human Thyroid Cancer Related to Over Expressed mir-221 and mir-146b, J Molaei, M Meidanchi, S Mirzaahmadi, J Mol Biomark Diagn S 2, 2, 2017.

16.Investigating the expression levels of miR-146b as a tumor marker for early diagnosis of thyroid cancer, Melika Maydanchi, Sina Mirzaahmadi, International Journal of Pharmaceutical Research & Allied Sciences 5 (3), 2016.

17.Comparative Study of Shigella flexneri Identiefication with Colorimetric Assay Based on Gold Nanoparticles and Electrophoresis Method, Sorraya Salimi, Mojtaba Salouti, Sina Mirzaahmadi, Rasoul Shokri, Ali Einloo, Biological Journal of Microorganism 5 (19), 53-62, 2016.

18.Evaluation of the frequency of RNASE L in 462 and 541 variants in Iranian prostate cancer patients, Sirweh Molla, Massoud, Ghaffarpour, Somayeh Saeeda, Seyed Werya Hossieni, Sina Mirzaahmadi, Mohammad Reza Rahmany, Cancer Research 74 (19 Supplement), 1848-1848, 2014.

19.Optimization of Bacillus Cereus Detection Based on HemolysinBL And Non Hemolytic Enterotoxin C Genes by Multiplex PCR, Kasra Sadeghi, Sina Mirzaahmadi, Iranian Journal of Public Health 43 (2), 291, 2014.

20.Coexpression of luxA and luxB genes of Vibrio fischeri in NIH3T3 mammalian cells and evaluation of its bioluminescence activities,, Golnaz Asaadi Tehrani, Sina Mirzaahmadi, Mojgan Bandehpour, Bahram Kazemi, Luminescence 29 (1), 13-19, 2014.

21.Investigation and identification of the nuc, fem B mecA and aac (6 )/aph (2 )-Ia genes in the staphylococcus aureus isolated from northwest Iran by multiplex PCR method, Mahdad Kiani Nia, Alka Hasani, Akbar Hasani, Yaeghob Sharifi, Sina Mirzaahmadi, Leila Deghani, Medical Journal of Tabriz University of Medical Sciences 35 (1), 68-73, 2013.

22.Identification of Anaerobic Bacteria in Iranian patients with Rheumatoid Arthritis, Shahb Shafeian, Mojgan Bandehpour, Sina Mirzaahmadi, Azadeh Tofighi, Novelty in Biomedicine 1 (2), 39-42, 2013.

23.Expression of recombinant human coagulation factor VII by the Lizard Leishmania expression system, Sina Mirzaahmadi, Golnaz Asaadi-Tehrani, Mojgan Bandehpour, Nooshin Davoudi, Leila Tahmasbi, Nahid Hosseinzadeh, Hasan Mirzahoseini, Kazem Parivar, Bahram Kazemi, Journal of Biomedicine and Biotechnology, 2011.

24.Molecular cloning and expression of the luciferase coding genes of Vibrio fischeri, Golnaz Asaadi Tehrani, Sina Mirzaahmadi, Mojgan Bandehpour, Faramarz Laloei, Akram Eidi, Toraj Valinasab, Bahram Kazemi, African Journal of Biotechnology 10 (20), 4018-4023, 2011.

25.Cloning of the luxA and luxB genes of Vibrio fischeri, Golnaz Asadi Tehrani, Sina Mirzaahmadi, Mozhghan Bandehpour, Faramarz Laloyee, Bahram Kazami, Akram Eidi, Tooraj Valinassab, Journal of Animal Environment 3 (2), 33-40, 2011.

Genetic Investigation of a Case of Acute Familial Mediterranean Fever (FMF) with the Onset in Neonatal period. Sina Mirza-Ahmadi, Safar Farajnia, Mandana Rafii, Mohammad Hoseein Soumi, Ailar Nakhlband, Hanieh Rezaii, Leila Mohammadnezhad, Journal of Advances in Medical and Biomedical Research 15 (58), 74-80, 2007.

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