Nikos Nicholas, MB (ASCP)
Clark, NJ 07066
*****.********@*****.***
Skills
A results oriented team player with comprehensive technical and supervisory experience in a time sensitive environment in molecular diagnostics.
Experience
October 2020-July 2021
Scientia Diagnostics/Trugenx Precision Medicine, Lyndhurst, NJ- Senior Molecular Technologist
●Utilized ThermoFisher QuantStudio 12 and Agena Massaray for RPP and PGx assays
●Performed assays and maintained Agena Massarray platform
●Operated APOLLO LIMS for test management and patient information
●Extracted nucleic acids from NP swabs using Kingfisher Flex 96 well platform
●Trained technologists on new platforms and efficient use of equipment
●Lowered costs through improved use of vendors and equipment
November 2011-August 2020
QDX Pathology Services, Cranford, NJ - Lead Molecular Pathology Technologist/Supervisor
●Key Operator trained on Hologic Panther random access test platform
●Conducted validations for LDTs on Panther, Luminex ARIES, and ELISA platforms
●Utilized Fisher QuantStudio 12k/Kingfisher with Chromacode Assay for SARS-CoV-2 detection
●Extracted nucleic acids from stool, blood, urine, NP/saliva and pap specimens
●Performed PCR, RT-PCR, signal amplification, TMA, and DNA probe diagnostic tests
●Ordered all lab supplies and consulted with executives on platform and product usage
●Released results after pathologist review
●Maintained lab equipment, liaison to FAS/Engineers
●Saved money through vendor optimization
March 2009-November 2011
Medical Diagnostic Labs, Hamilton, NJ- Lab Shift Coordinator
●Ensured prioritized and time sensitive tests were tracked and verified
●Verified technician analysis for result release
●Approved weekly payroll
●Coordinated lab inventory and ordering
●Prepared reagents and controls
January 2006 - March 2009
Medical Diagnostic Labs, Hamilton, NJ- Lab Technician
●Extracted DNA/RNA using Xtractor-Gene, Phenol-Chloroform, and Qiagen methods
●Cell culture of Candida species and UTI pathogens for antibiotic resistance
●Performed and analyzed real-time, RT-PCR and touchdown PCR manually and robotically.
●High competency on Luminex LX/MAGPIX, Real time PCR, Acoustic Liquid Handling, and Pyrosequencing platforms
●Interpreted DNA Fragment Analysis using Beckman Coulter CEQ 8000
Education
December 2019
Technologist certification in Molecular Biology from American Society for Clinical Pathology
August 1998-May 2003
West Virginia University - Bachelor of Arts, Biology