Entry Operator Research Technician

MARGARET KEILMAN Yardley, PA ***********@*****.*** 215-***-****

SENIOR RESEARCH ASSOCIATE

Virology Immunology Molecular Biology

Versatile, Experienced, Analytical and Hard working with an extensive medical research background in both academic and pharmaceutical settings. Looking to re-enter the work force in a position where I can apply my professional research experience and expertise. As well as utilize experience acquired outside of the professional work place environment raising a family, including care taking, teaching, interpersonal, mentor and leadership volunteer roles. Professional Skills

· Data Analysis and Instrumentation · Process Improvement · Study Design and Assay development

Laboratory Skills

· FACS, ELISPOT, ELISA, RNA/DNA isolation, RT PCR, cloning, DNA sequencing, site-directed mutagenesis, Reporter assays, mitochondrial toxicity assays, transient transfection assays, total RNA transfection assays, cell culture, PBMC isolation(Yicoll), aseptic technique.

EXPERIENCE

Domestic, Yardley, PA 2011 - Present

Full-Time Care Taker and Volunteer

Nanny and Care Taker of children and Parents, Girl Scouts Troop Leader, Tutor, Teaching Assistant Prep school, Homeroom parent and PTO; experience in organizing, running age-appropriate events and activities for Elementary school age children in School, Search Angel; Assist individuals seeking biological parents utilizing DNA matching and genetic genealogy, 2020 census 175

PHARMASSET, Princeton, NJ 2005 - 2011

Senior Research Associate, Biology Department

Research and Development of novel antiviral compounds against infectious viruses (HIV, HBV, HCV, FLU, DENGUE), including discovery and development of company’s breakthrough HCV therapeutic, PSI-7977 (SOVALDI). Designed and implemented various cell-based assays for assessing antiviral activity of nucleosides and small molecules in viral systems.

Developed and implemented infectious real time RT PCR assay to measure anti-HIV activity of newly synthesized compounds.

Designed and applied high throughput (dose response) screen (HTS) to measure anti-HCV activity of all newly synthesized compounds.

ModiYied toxicity assays (mitochondrial and lactic acid accumulation) which provide faster throughput toxicity assessment of lead antiviral drug candidates.

Established and organized of the BL2/3 level infectious and non-infectious biological laboratories.

Recognized cell cultures and relevant assays (HCV, HBV, cytotoxicity, mitochondrial toxicity) for Pharmasset when they moved into new facility in Princeton NJ.

VERTO INSTITUTE, Princeton, NJ 2004 - 2005

Senior Research Associate, Biology Department

Conducted Carcinoid and Neuroendocrine Tumor Research related to neuroendocrine tumors and carcinoids.

Researched and designed experiments to measure effect and characterized possible therapeutic potential of various peptide analogs, antibodies and cytokines in neuroendocrine tumors and carcinoids.

Studied and planned cell-based assays for determining intracellular signaling, receptor expression, and hormone and cytokine regulation in neuroendocrine tumor cell lines and transfected cell lines. EARLYCAREER

Research Associate, Immunotherapeutic Department - PURDUE PHARMA, Cranbury, NJ Immunologist, Viral Vaccine Department – MERCK RESEARCH LABORATORIES, West Point, PA Research Specialist, Department of Pathology and Laboratory Medicine – UNIVERSITY OF PENNSLYVANIA, Philadelphia, PA Research Technician – LYOPHILIZATION TECHNOLOGY, Warwick, PA MARGARET KEILMAN

EDUCATION&AFFILIATIONS

Bachelor of Science, Laboratory Sciences/Biotechnology Thomas Jefferson University, Philadelphia, PA Associates of Arts, Biology Bucks County Community College, Newtown, PA PUBLICATIONS

Available Upon Request

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