Associate Scientist

Jean K. Wang

*** *** **** **. *** ***, Escondido, CA 92029 714-***-**** **.*******@*****.***

Education

**** – Pres Johns Hopkins University M.S. Bioinformatics 2013 – 2015 University of California, Los Angeles (UCLA) B.S. Biochemistry 2009 – 2013 Moorpark College, Moorpark, California Biotechnology Professional Experiences

2020 – Pres Associate Scientist In Vivo Sciences Janssen – Johnson & Johnson Collaboration with teams from various therapeutic area to establish in Vivo models. Global colony management data analysis and process automation. 1. Collaborating with our biomarkers, immunology, neuroscience, and toxicology teams in establishing methodology and protocols and conducting pilot studies as well as identifying resources available to achieve study goals. 2. Working with our global operation leader to establish data collection and automatic analysis tools across global sites, which improved the data unity for downstream comparison as well as improved efficiency in data collection and analysis by 70-80%. 2017 – 2020 RA / Lab Manager Dhawan Lab Beckman Research Institute Resource planning and coordination between core facilities and team members. 1. Working with various cores to arrange and ensure availability of resources needed for studies, including reagents, materials, in vivo cohorts, etc. 2. Serve as a liaison between the principle investigators and postdoctoral researchers and other laboratory associates to ensure progresses of studies 2016 – 2017 Research Assistant Reiter Lab UCLA

Targeting and therapeutic methods for androgen independent prostate cancer. 1. Developed methods to trace prostate cancer cell marker (PSCA) for surgical and clinical purposes. [See: publication (2).]

2. Studied the behavior of androgen sensitive prostate cancer cell lines (e.g., the LNCaP cells) under androgen deprivation conditions, upon overexpression of a transcription factor of our interest, POU3F2. [See: publication (4).] 3. Studied the effect of a novel N-cadherin monoclonal antibody in combination with oral enzalutamide treatments in prostate tumor progression. [See: publication (5),

(6).]

2013 – 2015 Undergraduate Researcher Hong Wu Lab UCLA Transcriptional regulation of DNA damage repair mechanisms. 1. Studied DNA damage response and the repair mechanism upon the loss of Chd1, a chromatin remodeling enzyme. [See: publication (3).] 2. Studied the change in the nuclear expression of stem cell markers in prostate specific Chd1 KO animals via immunohistochemistry. [See: publication (10).] Skills

Project and Laboratory Managing skills

Teamwork and communication

Multitasking and planning

Data organization / Inventory establishment

Data visualization

Process improvement and automation

Systematic procedure development

Computer skills

Microsoft Office:

Word, Excel, PowerPoint, Access, OneNote, Form, SharePoint, Power Automate, etc. Programming languages: JAVA, Microsoft VB, LATEX

Research Tools: Prism, Photoshop, SnapGene, FinchTV DNA Tools, etc. Animal skills (rodent)

Strategic breeding and strain maintenance

Handling

Tag and biopsy sampling for genotypes

Intraperitoneal and subcutaneous fluid administration Oral gavage for fluid administration

Subcutaneous xenograft

Male physical castration

Blood sugar measurement and glucose tolerance test Necropsy

Molecular biology assays

DNA and RNA isolation, qualitative PCR, qRT-PCR

Cloning, DNA sequence analysis

Protein isolation, western blot

ELISA

Immunohistochemistry

Theory of ChIP and RRBS sequencing

Theory of CRISPR

Cell culture assays

Human and mouse primary pancreatic islet isolation and cultures Mouse primary, xenograft, and metastatic cancer cell isolation and cultures Drug treatment and antibiotic response studies

Migration and invasion assays

Stem-ness (sphere) assay

Seahorse assay

Language skills

English

Mandarin Chinese

Taiwanese

some Japanese

Publications

Manuscripts

2020 (1) Wang JK, Gurlo T, Rodnoi P, Rajkumar M, Butler AE, Dhawan S. DNA methylation regulates the diversity of pancreatic beta-cells. Diabetes. Manuscript in preparation. 2018 (2) Zhang M, Kobayashi N, Zettlitz KA, Kono EA, Yamashiro JM, Tsai WK, Jiang ZK, Tran CP, Wang C, Guan J, Wu AM, Reiter RE. Near-infrared-dye labeled anti-Prostate Stem Cell Antigen minibody enables real-time fluorescence imaging and targeted surgery in translational mouse models. Clin Cancer Res. 2018 Oct 9. pii: clincanres.1382.2018. doi: 10.1158/1078-0432.CCR-18-1382. 2017 (3) Shenoy TR, Boysen G, Wang MY, Xu QZ, Guo W, Koh FM, Wang C, Zhang LZ, Wang Y, Gil V, Aziz S, Christova R, Rodrigues DN, Crespo M, Rescigno P, Tunariu N, Riisnaes R, Zafeiriou Z, Flohr P, Yuan W, Knight E, Swain A, Ramalho-Santos M, Xu DY, de Bono J, Wu H. CHD1 loss sensitizes prostate cancer to DNA damaging therapy by promoting error-prone double-strand break repair. Ann Oncol. 2017 Jul 1;28(7):1495-1507. doi: 10.1093/annonc/mdx165. Abstracts

2018 (4) Kobayashi N, Hong D, Wang C, Yamashiro J, Guan J, Reiter RE. The role of POU3F2 in the aggressive behavior of neuroendocrine prostate cancer. AACR Annual Meeting. 2018 2018 (5) Kono EA, Kobayashi N, Zettlitz K, Li K, Yamashiro J, Wang C, Wu A, Reiter RE. Development of fully humanized N-cadherin monoclonal antibodies for treatment of castration resistant prostate cancer. AACR Annual Meeting. 2018

2017 (6) Kono EA, Kobayashi N, Zettlitz K, Yamashiro J, Wang C, Hu DZ, Wu AM, Reiter RE. AACR International Conference – Efficacy of new developed N-cadherin monoclonal antibodies in combination with enzalutamide against castration-resistant prostate cancer. AACR Annual Meeting. 2017

Presentations

2019 (7) Wang JK, Rodnoi P, Gurlo T, Dhawan S. De novo DNA methylation regulates beta cell diversity and function. Annual Rachmiel Levine-Arthur Riggs Diabetes Research Symposium. 2019

(8) Wang JK, Subramaniam R, Parveen N, Gurlo T, Dhawan S. Epigenetic regulation of beta-cell function and survival. Annual Rachmiel Levine-Arthur Riggs Diabetes Research Symposium. 2019 2018 (9) Wang C, Rodnoi P, Dhawan S. DNA methylation regulates insulin secretion in pancreatic beta cells. Annual City of Hope Poster Session. 2018

2015 (10) Wang C, Shenoy T, Wu H. Causal Relationship Between Chromodomain Helicase DNA-Binding Protein 1 (Chd1) Deletion and Mouse Prostate Tumorigenesis. Annual UCLA Science Poster Day. 2015

Extra Curriculum

Volunteering Experience:

2018 Pre-surgery assistant Department of Surgery, City of Hope 2013 Research Volunteer Hong Wu Lab, UCLA

Teaching Experience:

2018 Mentor – City of Hope DMRI Summer Research Program 2018 Mentor – Eugene and Ruth Roberts Summer Academy 2016 – 2017 Apprentice Coach – UCLA Competitive Club Sports References

Available upon request.

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