Yuyuan (Peter) Guo

*** ******** ***. *** *******, California 90024

Tel. 469-***-****. Email: adpv9f@r.postjobfree.com

Qualifications:

● Immunology training, the experiences of molecular techniques, Confocal microscope, ELISA

● Mammalian cell culture, lentivirus/adenovirus gene expressions, transgenic plant gene expression, prokaryotic cell expression, RNAi to silence a gene, animal models (transgenic mouse model)

● Biological regent treatment for autoimmune diseases, oral vaccine to prevent viral infections

● NIH Grant writing. Scientific writing

Professional Experience (skills):

05/2021- Project Assistant Scientist, Transplant immunology, UCLA, working on Knock in/out mice to study Gsk3b, MerTK and Axl role in Macrophages.

01/2020-05/2021 Consultant and senior scientist part time in Renaissance Dermatopathology LLC, Plano, Texas. Antigen design for clinical diagnostic test kit, HE4, AMA test kit. SARS-CoV-2 nucleic test and Next Generation Sequencing (NGS) to test pathogen 16S and 28S rRNA correlated to antibiotics resistance.

08/2018-09/2019 Senior Scientist, Quorum Innovation Biopharmaceutical Company, Sarasota, Florida, Q-PCR, Short peptide, HEKa cell and Human Keratinocyte culture; EX-Vivo immunology study and wet lab experience.

09/2012-06/2018 Associate Prof. in Xinjiang Medical School, China. Hepatitis B/E virus study, HEV oral vaccine design. Instructed graduate student, taught immunology and advanced immunological techniques.

06/2009-09/2012 Post-doctor II and Bio-Safety Level 2 lab manager in Rheumatology, Southwestern Medical Center, Dallas, Texas. Transgenic mouse techniques, genomic typing and lupus study: immuno-therapy, autoimmune disease immunotherapy, adenovirus and lentivirus expressed foreign genes. Type-I interferon study in autoimmune diseases, bone marrow cell culture, flow cytometry.

04/2008-06/2009 Research associate in Medical School, University of Vermont. Transgenic mouse model development, mucosal immune response and immune tolerance. Mouse embryo transplant. T cell labeled with RFP and confocal microscope technique.

11/2006-04/2008 Post-doctor in Molecular and Pathology Dept. Medical School, University of Wisconsin-Madison. Chemotherapy to cure prostate cancer, cancer mouse model development.

09/1997-10, 2006 Research Assistant in Pathobiological Science, University of Wisconsin-Madison . Animal viral infectious disease, viral oral vaccine, transgenic plant express animal viral genes. ELISA. Animal viral titration. Recombinants virus to express viral genes. Molecular gene cloning. IVF ( in vitro fertilization): bovine embryo and embryo culture for transplantation. Transplant immunology study: blocking costimulatory CD28/CTLA-4 with antibody. Scientific writing, such as grant and papers. Fetal Bovine Serum (FBS) production training.

09/1989-09/1997 Research Assistant in Dept. of Bio. and Biotech. Protein-protein interaction (LDH subunit interaction), Protein modification, enzyme catalytic center study, protein isolation with chromatography. Thymus peptide isolation and injection development. Calf rennin isolation to cure baby milk vomiting.

07/1984-09/1989 Research Assistant in Microbiology, Xinjiang Agriculture Academy. Fungi isolation, classification, virus isolation.

Education:

08/2001-10/2006 Ph.D. in Comparative Biomedical Sciences, Dept. of Path biological Sciences, University of Wisconsin-Madison

09/1997-08/2001 M.S. in Veterinary Science, Veterinary Medical School, UW-Madison 08/1989-03/1992 M.S. in Dept. of Bio. Sci. and Biotech. Tsinghua University, China

09/1980-07/1984 Bachelor of Agronomy on plant protection, Northwestern Agr, Univ. China

Patent:

Guo, Y. Method of Assessing DNA Mutability, 2011, Guo, US. Patent No. 7,981,670.

Guo, Y. An Adjuvant Effect on CTL Response in HIV Vaccine (pending).

Publication (Chinese publication was not listed.):

1. Hanwen Zhang1,2#, Ming, Ni1, 3#, Jing Zhang1, Han Wang3, Yuyuan Guo1, Dan Jin1,4, Ronald W. Busuttil1, Jerzy W. Kupiec-Weglinski1, Wei Li2, Xuehao Wang3 and Yuan Zhai1*Gsk3b regulates the resolution of liver ischemia reperfusion injury via MerTK. JCI (Peer review)

2. Guo, Y. Schultz, R. 2021. Short term experiment in Escherichia coli tells mutagenesis is sequential (Scientific Report: peer review).

3. La Xiaolin, Zhang Fengbo, Li Yanhua, Li JUn, Guo Yuyuan, Ding Jianbing. 2015 Upregulation of PD-1 on CD4+CD25+ T cells is associated with immunosuppression in liver of mice infected with Echinococcus multilocularis. International Immunopharmacology. 26 (2).

4. Yanhua Li, Fengbo Zhang, Mohammed H. Althunayan, Xiao-An Hu, Yan Xin, Haiying Jia, Yuyuan Guo, Xiumin Ma, Hao Wen, Jianbing Ding. 2013. Construction and identifcation of the recombinant plasmid, pET30a-EgA31-Eg95 of Echinococcus granulosus. Experimental and Therapeutical Medicine.

5. Wu T. Du Y. Han J. Singh S. Xie C. Guo Y. Zhou XJ Ahn C. Savena R. Hohan C. Jan 23 2013. Urinary Angiostatin-a novel marker of renal pathology chronicity in lupus nephris. Mol Cell Proteomics.

6. Guo, Y. Orme, J. Mohan, Chandra. 2012. A Genopedia of Lupus Genes-Lessons from Gene knockouts. Current Rheumatology Reviews.

7. Guo, Y. Laurie, Larson, Thomas L. German, Ronald D. Schultz. 2011. Glycosylation on BVDV gp53 is critical for its immunogenicity. June, 9 th Food and Healthy abstract.

8. Guo, Y. German, T., Schultz, R. 2007. A cryptic promoter in potato virus X vector interrupted plasmid construction. BMC Mol. Biol (8): 17.

9. Wattiaux, M. A. Frank, G. G. Powell, J. M., Wu, Z., Guo, Y. 2002. Agriculture and dairy production systems in China: An overview and case studies. Babcock Institute Discussion Paper No. 2002-3.

Honors and Awards:

1988 Advanced Scientific Award in Xinjiang Agriculture Science, Xinjiang, China. 1990 Guanghua Scholarship for outstanding student, Beijing, China. 2006 WARF Patent Award, Madison, Wisconsin.

Training and skills

● Flow cytometry training, summer, 2007 Madison, Wisconsin. ● Transgenic mouse manipulation training, spring in 2008, Burlington, Vermont. ● ELISPOT training, spring, 2008, Burlington, Vermont. ● Bio-Safety Level 2 laboratory training. ● Stem Cell advanced biology, spring in 2011, Dallas, Texas. ● Computer skill: Word Processing, Microsoft Office, and Analysis Skills.

Referees:

James Davie, MD and Ph.D. Renaissance Dermatopathology LLC, Plano, Texas. Email: adpv9f@r.postjobfree.com; Tel. 412-***-****.

Ronald D. Schultz, Professor and Chairman in the Pathobiological Sciences Dept. Vet. Med. School, UW-Madison. Email: adpv9f@r.postjobfree.com Tel: 608-***-****.

Yong Du, Assistant Professor in the Health Center, Pen. State. Email: adpv9f@r.postjobfree.com Tel. 717-***-****.

Yuan Zhai, Professor, Department of Surgery, UCLA Email: adpv9f@r.postjobfree.com Tel. 310-***-****.

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