CURRICULUM VITAE

Mr. Amol M Kanampalliwar

Infectious Disease Lab

Institute of Life Sciences

Bhubaneswar, India-751023

E-mail: adpc8r@r.postjobfree.com

Ph: +918*********

Research Interest

Host microbe interactions, human microbiome, Biofilm related infections, antibiotic resistance Research Experience

Institute of Life Sciences, Bhubaneswar, India

1. Ph.D. (2014-2020)

Supervisor: Dr. Durg Vijai Singh

Institute of Life Sciences, Bhubaneswar, India

Thesis title “Studies on biofilm matrix of Vibrio cholerae”

Molecular typing of V. cholerae strains isolated from various regions of India to determine their clonal Propagation (clinical and environmental isolates).

The biofilm studies were focused on eDNA and its interaction among various component of biofilm.

Detection of eDNA release during the growth of bacteria and effects of DNase I on biofilm in time dependent manner, here we showed that eDNA is released at late log phase of bacterial growth and DNase I affects the biofilm when treated at initial time point (t=0).

As VPS is important biofilm component of V. cholerae we investigated the interaction among eDNA and VPS through various techniques, similarly we also investigated the interaction among OMVs and eDNA.

The interaction of eDNA with other components of biofilm gives strength to biofilm, here we have shown that eDNA colocalized with VPS and interacts with it. The confocal analysis showed that eDNA is present all overthe biofilm as eDNA clouds.

Physiological consequences of eDNA and OMVs on biofilm formation/colonization was also determined in mouse model with biofilm bacteria and planktonic bacteria, here we found that biofilm bacteria have more colonization as that of planktonic bacteria and OMVs increase the colonizationof bacteria as compared to eDNA. 2. Collaborative projects

Pregnancy-associated human listeriosis: Virulence and genotypic analysis of Listeria monocytogenes from clinical samples (2015), Journal of Microbiology 53

(9), 653-660(data collection and analysis)

3. Project trainee on “Drug Screening against COVID-19”. 4. Project coordinator “Genome Surveillance of COIVD-19”. Publications

1. Kanampalliwar A, Singh DV (2020), Extracellular DNA build and interacts with vibrio polysaccharide in the biofilm matrix formed by Vibrio cholerae. Environ Microbiol rep. 12(5), 594–606

2. Kanampalliwar, A. & Singh, D. V. (2020), Virulence Pattern and Genomic Diversity of Vibrio cholerae O1 and O139 Strains Isolated from Clinical and Environmental Sources in India. Frontiers in Microbiology, 11, 1838. 3. Kanampalliwar AM (2020), Reverse vaccinology and its applications. Methods Mol Bio. 2020;2131:1-16

4. Kanampalliwar AM, Rajkumar S, Girdhar A, Archana T (2013), Reverse Vaccinology: Basics and Applications. J Vaccines Vaccin 4: 194

5. Kanampalliwar AM, Rajkumar S, Girdhar A, Archana T (2013),Web Based Tools and Databases for Epitope Prediction and Analysis: A Contextual Review, International Journal of Computational Bioinformatics and In Silico Modeling, Vol. 2, No. 4: 180-185

6. Kanampalliwar AM, Girdhar A, Arya R, Saxena R and Tiwari A: In silico epitope prediction and homology modeling of envelope surface Glycoprotein gp160, precursor region from Human Immuno Deficiency Virus -1. Int J Pharm Sci Res 2014; 5(7): 1000-24.

Core competencies

Animal skills: Disease model of V. cholerae, intestinal colonization of mice.

Cell Culture: Primary cell culture (vero) & drug screening against Covid-19, viral transfection,

Immunological and imaging techniques: Flow Cytometry, MTT assay, Fluorescence microscopy, confocal microscopy, SEM, TEM, AFM.

Biochemistry and molecular biology: Gene Cloning, PCR, DNA & RNA Isolation, cDNA library preparation, qPCR, DNA sequencing, Fluorescence Spectrophotometry, Electrophoresis, Western Blotting, Immunoprecipitation, ITC, affinity chromatography, EMSA, Extraction and quantitation of the protein. NGS

Software and Bioinformatics: Graph Pad Prism, Roche LC480, End Note, Zeiss LSM browser, Image J, Corel Draw, Primer designing and basic bioinformatics tools Fellowships and Awards

GATE-2011

ICAR-SRF-2014

Education

Exami-

nation

Year of

passing

University/Board Specialization Division

Ph.D Thesis

submitted

Utkal University Biotechnology -

M.

Tech

2013 RGPV, Bhopal Biotechnology First

M. Sc. 2011 RTMNU, Nagpur Biotechnology First

B.Sc 2009 RTMNU, Nagpur Biotechnology First

References

1. Dr. Durg Vijai Singh

Scientist G

Institute of Life Sciences

NALCO Square, Bhubaneswar, Odisha

Email: adpc8r@r.postjobfree.com, adpc8r@r.postjobfree.com

2. Dr. Sunil Kumar Raghav

Scientist E

Institute of Life Sciences

NALCO Square, Bhubaneswar, Odisha

Email: adpc8r@r.postjobfree.com,adpc8r@r.postjobfree.com 3. Dr.Rupesh Dash

Scientist E

Institute of Life Sciences

NALCO Square, Bhubaneswar, Odisha

Email: adpc8r@r.postjobfree.com, adpc8r@r.postjobfree.com

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