Hien-Anh NGUYEN, PhD

adnoe3@r.postjobfree.com

linkedin.com/in/nguyenhienanh

Associate Director: cell-based assays and high-throughput small molecule screening Principal Scientist: 7 years of experience in pre-clinical biologic development process

• Associate Director at SAMDI Tech Inc., USA 2020-2021 My mission consists of building a brand new cell culture facility from scratch that offers both i) conventional cell-based assays and ii) unprecedented mass spectrometry-amenable cell-based assays for a high-throughput label-free screening of millions of small molecules in a complex biological context as cultured cells within several weeks. Achievements: A fully functional new CRO offering >20 cell-based assay services; initial high-throughput screening of 100,000 compounds

• Principal Investigator/ Chief Scientific Officer at Enzyme by Design Inc., USA 2017-2020 During 3.5 years at EbD, I successfully competed and was awarded with three Phase I and one Phase II SBIR/STTR small business grants. I led the in-house EbD's protein engineering and discovery programs as well as the sourcing of multiple CROs/CDMO for drug manufacturing and pre-clinical drug evaluation programs for IND enabling studies. Beyond the technical leading role of a CSO, I led the management team to complete interviews with KOLs, physicians, nurses and hospital staffs in leading cancer centers throughout the US to confirm the clinical & commercial needs for developing our biologics drug candidates. Achievements: $3.5M in funding; >130 customer discovery interviews; a throughout 2-year plan for drug CMC and IND enabling studies

• Scientific consultant at Tango Biosciences LLC., USA 2018-2019 My job consists of setting up a brand new CHO culture facility and providing scientific consulting services on mammalian protein production of large-scale quantity and of high purity levels for Tango Biosciences to feed in its phase-display affinity selection downstream.

Achievements: A fully functional new CHO facility capable of transiently expressing multiple difficult protein targets

• Senior Scientist at University of Illinois at Chicago, USA 2013-2020 I initiated and led multiple fundamental and translational projects aimed at gaining an increase in understanding of the relationship between protein structure and function towards oncology bio-better drugs. I spent 3 years to master crystallography skills from gene/protein engineering to crystallization and structure refinement, identifying 2 lead drug candidates. I then spent the next 4 years to characterize these drugs in vitro test tubes and in culture, together with in vivo PD/PK/ADME evaluation. Overall, along with a solid and board technical skillset, I naturally refined my project management, grant writing and supervision skills. I enriched my experience in critically evaluating the scientific literature and adopted novel technologies which facilitate drug discovery. Achievements: 1 issued international patent and 1 pending PCT patent applications, 7 peer-reviewed publications.

• Bio-analyst and Pathway constructor at Institute Curie, Paris, FRANCE 2012-2013 Project: “From Atlas of Cancer Signaling Networks to druggable target design” https://acsn.curie.fr I was in charge of creating an open access & comprehensive signaling map of the Epithelial-to-Mesenchymal Transition process. I was able to deliver the map after 10 months (a process normally requires three to four years of full time work). The map of more than 5,000 genes and proteins with Google’s navigation principle is online at https://acsn.curie.fr/navicell/maps/emtcellmotility/master/index.html Overall, I developed Computational pathway analysis and Bioinformatics skills, along with competences in quickly evaluating and summarizing big data, in making conclusions that clarify next steps towards achieving project goals. I also experienced working in close collaboration with physicians from the Rene Huguenin hospital. Achievements: 1 peer-reviewed publication recommended by F1000Prime http://f1000.com/prime/725660252; 1 open-access database.

• Research Associate: PhD fellow at CEA – Institute of Structural Biology, Grenoble, FRANCE 2008-2012 Project: “Discovery of a new bacterial protein kinase family: functioning mechanism & cellular roles” I worked with a new enzyme, target for antibiotic development, but of unknown function. My mission was to characterize this enzyme biochemically and to find its real cellular functions in vivo. Overall, I obtained experience working in a European research project of multiple partners. I developed sough molecular biology and biochemistry skills as well as deep knowledge in bacterial protein kinases including 3.5-year experience in radioactivity kinase assay. Achievements: 1 peer-reviewed article; 1 invited talk and multiple poster presentations

• Research Assistant: Master 2 at AgroSup Dijon – Bourgogne University, FRANCE 2007-2008 Project: “Influence of culture and chilling kinetics on the stability of lyophilized sourdough starters” I optimized the lyophilization process for an industrial bacterium responsible for malolactic fermentation in wine-making. Overall, I obtained experience working in food industry along with skills in Microbiology and cell culture. 2008-2012 PhD in Biochemistry at Grenoble University, France Mention: summa cum laude (with distinction)

2007-2008 Master in Biotechnology – Food Science, Sensory and Consumer Research at Bourgogne University, France Mention: magna cum laude (3rd ranking in class of 30 students) 2002-2007 Engineer in Biotechnology – Food Technology at Hanoi Polytechnic University, Vietnam Mention: summa cum laude (1st ranking in promotion of >4800 students), Vietnamese grade 9.0/10 ~ U.S.A GPA 4.0 2002 Bachelor of Science in Chemistry, Excellence high school attached to Natural Science University, Vietnam Mention: magna cum laude – great honor

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Languages Vietnamese (mother tongue), French (bilingual – acquired citizenship), English (fluent) Music Pianist at Hanoi Conservatory of music for 11 years (1991-2002) Technique High throughput small molecule drug screening: from compound management and automated liquid handling to compound screening campaigns using SAMDI mass spectrometry or fluorescent readouts. Matrix PlateMate, TTP Labtech Mosquito, Multidrop, Nanodrop, MALDI Ab Sciex Tof/Tof, PHERAStar FS and FSX Biochemistry: from protein engineering and large-scale production (both bacterial and mammalian (ExpiCHO) expression systems) to physical and enzymatic characterization. TFF, HPLC, FPLC (AKTA), SEC, DLS, Biacore/SPR, CD, TSA, SDS-PAGE, Western Blot, ELISA, multiple in-vitro enzymatic assays Crystallography: from protein crystallization to X-ray data processing and structure determination. Home-source X-ray; APS and ESRF synchrotrons, XDS and HKL3000 packages; Pymol, Chimera Bioinformatics: from molecular signaling map building (>5,000 proteins and genes) to pathway analysis. Cytoscape 3.0, CellDesigner 4.0, BiNome 3.0 softwares; Python, R languages (not developer but advanced user) Molecular biology: from inactivation of chromosomal gene to cloning and gene engineering. PCR, gene deletion/insertion/point mutations, chromosomal gene knock-out, CRISPR/Cas9 genome editing Microbiology: bioprocess using bacterial or yeast fermentation. E. coli (different strains), Bacillus subtilis, Oenococcus oenie, Lactobacillus acidophilus, Saccharomyces cerevisiae Cellular biology: setting up mammalian tissue culture facilities for 2 startups, master >20 cell-based assays Cultured >20 cancer cell lines, advanced user of BioTek and BMG microplate readers, CellCyte label-free imaging Animal: working with mice, rats, dogs for PK/PD and preclinical toxicological assessment towards IND applications i.p and i.v injection, cardiac puncture, tissue dissection, IVIS-live imaging, pharmacokinetic and pharmacodynamics Supervision Principal Investigator in 4 NCI SBIR grants (Phase I + Phase II), leading a team with 1 senior Postdoc and 4 technicians Supervised 1 MD/PhD student, 2 PhD students, 1 senior + 7 junior technicians and 3 undergraduate students Trained multiple teams on IVIS-live imaging and other techniques in working with rodents. Management Project management: Maintain 2 main jobs (45% and 55% FTE) and volunteering work between 2017-2020 CRO management: Conceive SOWs, manage Champions Oncology, Jackson Lab, Charles River Lab on multiple projects Business management: Co-founder; and Business developer for three different startups; Quote proposals for clients First author in 5 peer-reviewed articles

1. 2018, Cancer Res, 78, 1549-60, «A Novel L-Asparaginase with low L-Glutaminase coactivity Is highly efficacious against both T- and B-cell Acute Lymphoblastic Leukemias In Vivo»

2. 2017, J Mol Biol, 429, 3056-74, «Expanding the kinome world: a new family of protein kinase widely conserved in bacteria» 3. 2017, Sci Reports, 7:41643, «The differential ability of asparagine and glutamine in promoting the closed/active enzyme conformation rationalizes the Wolinella succinogenes L-asparaginase substrate specificity» 4. 2016, J Biol Chem, 291, 17664-76, «Design and characterization of Erwinia Chrysanthemi L-Asparaginase variants with diminished L-Glutaminase activity» 5. 2016, Biochemistry, 55, 1246-53, «Structural insight into substrate selectivity of Erwinia chrysanthemi L-asparaginase» Co-author in 6 peer-reviewed articles, 2 more en route in 2021 1. 2021, Blood Adv, 5, 1963-76 «The spleen as a sanctuary site for residual leukemic cells following ABT-199 monotherapy in ETP-ALL» 2. 2020, Curr Microbiol, 77, 4063-71, «UbK is Involved in the resistance of Bacillus subtilis to oxidative stress» 3. 2017, Sci Reports, 7:10224, «Discovery of human-like L-asparaginases with potential clinical use by directed evolution» 4. 2015, Oncogenesis 4, e160, «Atlas of Cancer Signaling Network: navigating cancer biology with Google Maps» 5. 2014, J Biol Chem, 289, 33175-86, «Identification and structural analysis of an L-asparaginase enzyme from guinea pig with putative tumor cell-killing properties»

6. 2014, J Med Chem, 57, 9480-94, «Structure-guided development of deoxycytidine kinase inhibitors with nanomolar affinity and improved metabolic stability»

1. Int. Patent. Appl. PCT/US2017/020090 “L-Asparaginase Variants and Fusion Proteins with Reduced L-Glutaminase Activity and Enhanced Stability”. https://patents.google.com/patent/WO2017151707A1/en 2. Int. Patent. Appl. PCT/US2018/046196 “Truncated Guinea Pig L-Asparaginase Variants and Methods of Use”. https://patents.google.com/patent/WO2019032952A1/en 2019 Among 40 HALO scientist awardees under the age of 40 Among 25 AnitaB PitCHER semi-finalists

2018 ASPIRE award nominee, “Smart Technologies for Healthy Societies” theme, for APEC scientists under the age of 40 AACR Cancer Research Early Career Award nominee

2017 Among 7 Finalists in UIC Startup Challenge (Presenter, Apr 10th) 2nd place in Fast Pitch Fan Favorite at IBIO-Index (Presenter, Apr 21st) 2016-2017 Among 5 Professional Entrepreneurship Awardees funded by Women In Bio organization 2008-2012 Among 20 Irtelis Awardees for PhD Research, fully funded fellowship by the Life Sciences Program of CEA (Commissariat à l'Energie Atomique et aux Energies Alternatives)

2007-2008 Among 25 Evarist Galois Awardees for Master Research, fully funded fellowship by the Embassy of France in Vietnam 2007 2nd prize in “National Students & Research Competition” of the Vietnam Ministry of Science and Technology 1st prize in “Students & Sciences” of Hanoi Polytechnic University 1st laureate of the 47th Engineering promotion of Hanoi Polytechnic University (Valedictorian speech) 2006 Honors Fellowship from Odon Valley, Rencontre du Vietnam Organization for outstanding scholar activity Honors Fellowship from Former Polytechnic Students Organization for top 10 engineers (attributed every 5 years) 2004 Agence Universitaire Francophonie Fellowship for French culture and languistics 2002-2007 5-year scholarship for top 5 engineer candidates of Hanoi Polytechnic University 199*-****-**-year scholarship for top 10 pianist students of Hanoi Conservatory of music Hien-Anh NGUYEN

+1-309-***-**-**

linkedin.com/in/nguyenhienanh

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