Scientist Biochemistry, Assistant professor

Dr. Mohammad Sadik

[PhD (Molecular and Structural Biology), M.Sc. (Biochemistry)]

Senior Research Fellow

CSIR-CDRI, Lucknow

E-mail: **********@*****.***

Phone: 730-***-****

I am looking for a faculty/research scientist position in colleges/organization in the area of proteomics along with the teaching opportunity to trend the graduate students in Biochemistry and Molecular Biology.

Course Subject Institution Year Result

PhD

Biochemistry and

Molecular Biology

CSIR-CDRI 2021 Completed

M.Sc. Biochemistry AMU, Aligarh 2013 68.20%

B.Sc. (Hons.) Biochemistry AMU, Aligarh 2011 72.26% Intermediate Science (Biology) A.J.I.C. Tanda 2006 75.20% High School Science A.J.I.C. Tanda 2004 75.16%

Qualified CSIR-JRF (NET), June 2014 (AIR-56, Reg. no. 326327). Ph.D.- “Analysis of putative nuclear-encoded proteins involved in the biogenesis of iron–sulfur clusters in Plasmodium falciparum mitochondrion” (Supervised by Prof. Saman Habib, Chief Scientist, CSIR-CDRI, Lucknow).

M.Sc.- Studies on Uric acid induced modification of Alpha-2- macroglobulin. (Dr. Fahim Halim Khan, Asst. prof., AMU, Aligarh)

Career Objective

Academic Qualification

Professional Qualification

Thesis Titles

Research Expertise

Presenting research work in various national and international conferences.

Presenting research paper as well as research work in the lab meetings and departmental seminar.

Training master’s student to complete their dissertation report.

Guiding other junior research fellows and designing experiments

Strong organizational skills and proficient with MS office tools

Good written and oral communication skills

Molecular Biology tools: Primers design, PCR amplification, colony PCR, Restriction digestion and ligation of plasmid, DNA/RNA isolation, Molecular cloning, Expression and purification of recombinant proteins, Analysis of proteins by SDS-PAGE, Blue- Native PAGE, Western blotting, Chemical crosslinking, protein-protein interaction (Pull- down assays).

Biochemistry tool: Enzyme kinetics assays including ATPase assay, Aconitase assay, Cysteine desulfurase assay, Conversion to apo-form, [Fe-S] cluster reconstitution, enzyme-mediated [Fe-S] cluster assembly, [Fe-S] cluster transfer assay under anaerobic condition, Iron binding assay, Metal content analysis, Organellar fractionation, Silver staining, Bacterial ribosome purification.

Immunological tools: Immuno-blotting, Antibody raising/purification, Immuno- fluorescence assay, Co-immuno-precipitation.

Cell culture: Maintenance of P. falciparum culture in vitro.

Imaging tool: Confocal microscopy (for localization of protein in P. falciparum) and light microscopy (to monitor life cycle stages of parasite).

Computational Biology: Hands on experience in bioinformatics tools like Modeller, Phyre2, Swiss-modelling for homology modelling, various visualizations software like PyMOL, Chimera, Leica LASX. Experience in various online bioinformatic software like Blast, Clustal, Expasy, Espript3, Plasmodb, Uniprot etc.

Biophysical tools: Affinity chromatography, Size exclusion chromatography (AKTA purifier system), UV-visible Spectroscopy, CD-spectroscopy.

Structural tools: Small angle X-ray Scattering (SAXS). Experiences

Publications

Conferences Presentation/ Workshop Attended

Animal handling: Raising antiserum in rabbit and mice.

Miscellaneous: Experience in designing experiments and guiding other fellows independently, Presenting research work in lab meetings as well as in departmental seminar, Experience in various software like MS-Office, Prism, and Origin etc.

Sadik M, Afsar M, Ramachandran R, Habib S. [Fe-S] cluster biogenesis and unusual assembly of the ISC scaffold complex in Plasmodium falciparum mitochondrion

(Molecular Microbiology in press).

Charan M, Choudhary HH, Singh N, Sadik M, Siddiqi MI, Mishra S, Habib S. [Fe-S] cluster assembly in the apicoplast and its indispensability in mosquito stages of the malaria parasite. FEBS J. 2017 Jul 11.

Afsar, M*., Shukla. A*., Bharti. S., Kumar. N., Sadik. M., Kumar. R., Kumar. S., Habib, S., Manju.Y., and Ramachandran. R., (2021) Role of Ribosomal protein RpsC in surveillance of damaged mRNA on the prokaryotic ribosome. (Manuscript under preparation). (* Equal contribution).

Mohammad Sadik and Saman Habib. Investigation of mitochondrial [Fe-S] cluster synthesis in Plasmodium falciparum. Current Trends in Drug Discovery Research

(CTDDR) -7th International Symposium, CSIR-CDRI, Lucknow (2019).

Attended a workshop on Basics of Small Angle X-ray Scattering (SAXS) held on May, 2019 at CSIR-CDRI, Lucknow.

Attended an international conference on Malaria parasite biology: Strategies for drug and vaccine development held on Nov-Dec, 2017; ICGEB, New Delhi.

Attended a National conference on Emerging trends in Biochemical and Toxicological Sciences, held on March, 2012, A.M.U Aligarh.

References

Prof. Saman Habib Dr. Amogh Anant Sahasrabuddhe

Dr. Niti Kumar

E-mail: *********@*****.***

Senior Scientist

Molecular Parasitology and Immunology Division

CSIR-CDRI, Lucknow, India

Declaration: I hereby declare that the particulars furnished herein by me are true to the best of my knowledge and belief.

Personal:

Father’ name Mr. Mohammad Nehal

Mother’ name Ms. Madeena Khatoon

Date of Birth 11/01/1989

Sex Male

Marital Status Unmarried

Nationality Indian

Permanent address Sakrawal East, Tanda, Ambedkar Nagar, UP, India. Mohammad Sadik

E-mail: *******************@****.***.**

Principal Scientist

Molecular and Structural Biology

Division, CSIR-CDRI, Lucknow, India

(PhD Supervisor)

E-mail: *****.*****@*****.***

Chief Scientist, Molecular and Structural

Biology Division, CSIR-CDRI, Lucknow, India

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