Plant Et

CURRICULUM VITAE

Name : Harbaoui

First Name : Hanène

Date and place of birth: 06-12-1981, Tunisia.

Nationality : Tunisian

CIN : 07084228

Adress : 05 Rue Saad El Bennani, 2055 Bir el Bey. Tunisia

Tél : 002**-********

E-mail : ***************@*****.***

STUDIES CONDUCTED & DIPLOMAS OBTAINED:

DIPLOMAS OBTAINED:

*Bachelor: Section: Experimental Sciences, June 2000; Session: Primary, Grade: fairly well, Location: Tunisia

*University Diploma of Scientific Studies (Baccalaureate + 2): Life Sciences, June 2002 (primary session); Grade: fairly well; Location: Faculty of Sciences of Tunis

*Degree (Baccalaureate + 4): Specialty: Life Sciences Option: Biotechnology, June 2004 (primary session); Grade: fairly well; Location: Faculty of Sciences of Tunis

*Master's degree (Baccalaureate + 6 years): Specialty: Genetics and Molecular Biology,

June 13, 2006, Grade: Very well; Location: Faculty of Sciences of Tunis

STAGES :

year

Traineeship

Establishment

Domain Search

2005-2006

Traineeship of master

Laboratory of Molecular Genetics, Immunology and Biotechnology, Faculty of Sciences of Tunis

Morphological identification & Molecular and Toxicological studies on a few species of cereal aphids in Tunisia

2006-2007

Traineeship

Laboratory of Molecular Genetics, Immunology and Biotechnology, Faculty of Sciences of Tunis

Study of the genetic structure of two species of cereal aphids (S. avenae and R.padi) and their viruses inoculated according to the host plant and geographical location in Tunisia

2007-2011

Stage jointly supervised

- UMR-BiO3P, Research Unit. Biology of Organisms and Populations applied to Plant Protection, INRA, Agrocampus western Rennes, France.

Study of the genetic structure of two species of cereal aphids (S. avenae and R.padi) and their viruses inoculated according to the host plant and geographical location in Tunisia

RESEARCH ACTIVITIES:

As part of the preparation of the Master (exposed in June 13, 2006) entitled: Morphological Identification & molecular and toxicological studies of some species of cereal aphids in Tunisia. Firstly, it was to identify on the basis of morphological criteria the main species infesting cereal crops in Tunisia. Secondly, Conducting toxicological tests performed on species with the highest density were being able to select resistant strains. Finally, it was to study the genetic variability of three aphid species by PCR-RAPD. The different results are interpreted by the appropriate statistical analysis by the PHYLIP software.

As part of preparing a Traineeship in Biological Sciences at UMR-BiO3P, (Research Unit. Biology of Organisms and Populations applied to Plant Protection, INRA, France) entitled:

Study of genetic structuring of two species of cereal aphids (S. avenae and R. padi) and their viruses inoculated depending on the host plant and geographical location in Tunisia. it was to:

- Determine the selective factor responsible for the genetic structure of populations of species Rhopalosiphum padi and Sitobion avenae using microsatellite markers (article submitted).

- Assess the diversity of plant populations infested by BYD viruses sampled on different cereals by serological tests (TAS ELISA), RT-PCR and sequencing. So to test the hypothesis that natural BYDV viral populations diverged with the type of host plant species cultivated.

MASTERED TECHNIQUES :

Extraction of nucleic acids.

Amplification by PCR-RAPD.

Techniques for electrophoresis.

Toxicological tests.

The microsatellites.

PCR-RFLP.

Elisa tests.

Computer literacy and bioinformatics:

- Word, Excel, Power Point, Internet Explorer, Access ...

- Statistical softwares: Phylip, TreeView, Genepop, Genetix, Population, Structure, Baps, fstat, Gimlet, Airlequin ...

Language Proficiency: Arabic, French, English (read, spoken and written).

PUBLICATIONS:

- Article accepted:

HARBAOUI, H., MEZGHANI, K.M., MARRAKCHI, M., MAKNI, M., et MAKNI, H., Morphological and toxicological study of cereal aphids (Homoptera, Aphididae) in Tunisia. Bulletin de la Société Zoologique de France, 2008, 133(1-3) : 203-213.

PATICIPATION IN SCIENTIFIC MEETINGS

* NATIONAL

- 17th biological meeting of ATSB. March 2006, Tunisia. Poster:

HARBAOUI H, MEZGHANI K.M, MARRAKCHI M et MAKNI H., Identification morphologique et étude de la variabilité par les marqueurs RAPD des espèces de pucerons de céréales en Tunisie.

- 18th biological meeting of ATSB and 2nd biological meeting of AMB. March 2007, Tunisia. Poster:

HARBAOUI H, MEZGHANI K.M, MAKNI M, MARRAKCHI M ET MAKNI H. Etude de la prévalence des espèces de pucerons des céréales identifiées en Tunisie.

- 19th biological meeting of ATSB et 3rd biological meeting of AMB. March 2008, Tunisia. Poster :

HARBAOUI HANEN, BOUKTILA D, MEZGHANI M, MARRAKCHI M, MAKNI M, PLANTEGENEST M, SIMON J.C ET MAKNI H. Etude des facteurs structurant la diversité génétique des populations Tunisiennes de Rhopalosiphum padi (Homoptères : Aphididae) par les marqueurs microsatellites.

- 20th biological meeting of ATSB et 5th International meeting of Biotechnology (ATSB-AMB). March 2009, Tunisia. Oral Communication :

HARBAOUI H, MEZGHANI K.M, MAKNI M, MIEUZET L, DEDRYVER CA, PLANTEGENEST M, SIMON J.C ET MAKNI H. Mise en évidence de flux géniques entre les populations de puceron Sitobion avenae colonisants les plantes hôtes cultivées et non cultivées en Tunisie.

* INTERNATIONAL :

- 3rd French Congress of Zoology in Tabarka. November 2006, Tunisia. Oral communication:

HARBAOUI H, MEZGHANI K.M, MARRAKCHI M et MAKNI H. Etude toxicologique de deux espèces de pucerons des céréales en Tunisie.

- AFPP – 8th International Conference on Pests in Agriculture 22 and October 23, 2008, Montpellier-France. Oral communication :

HARBAOUI H, MEZGHANI M, MAKNI M, MIEUZET L, DEDRYVER CA, PLANTEGENEST M, SIMON J.C ET MAKNI H. Mode de reproduction et structuration génétique des populations tunisiennes du puceron des céréales Rhopalosiphum padi.

- 7th International Francophone Congress of Entomology (CIFE, 2010, Louvain, Belgique) : Oral communication:

HARBAOUI HANENE, SIMON JEAN-CHRISTOPHE, DEDRYVER CHARLES-ANTOINE, MAKNI MOHAMED, MAKNI HANEM1& PLANTEGENEST MANUEL. Structuration génétique des populations du puceron des céréales Rhopalosiphum padi dans un agrosystème particulier : oasis du sud tunisien

PARTICIPATION IN SCIENTIFIC WORKSHOPS

- Participation in Workshop « First Tunisian-German Workshop on Bioinformatics » Tunisia, September 2006.

- Participation in the practical workshop "New Tools for Making and Control in Molecular Biology" Institut Pasteur of Tunis, February 2008.

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