Plant Assistant
Resume:
ANTON KARNOUP
Scientist II, Nektar Therapeutics
********@*******.***, ********@******.***
SUMMARY
Best fit for a position as a protein scientist with emphasis on mass spectrometry (protein structure, characterization of sequence and PTMs, quantitation, purification, production, QC/QA), or a bioanalytical specialist (analytical methods and functional assay development, run analyses, etc) in biotechnology and biopharmaceutical industry, institutional or academic core facility, or a contract laboratory.
EDUCATION
Master of Science in Analytical Chemistry 1997–2000
University of Pittsburgh Pittsburgh, PA
Thesis: “Alpha-Helix Peptide Folding and Unfolding: A Nanosecond UV Resonance Raman Study”
Master of Science in Protein Science 1993–1995
Biochemistry and Molecular Biology Pushchino, Russia
Moscow State University, Branch of Department of Molecular Biology at Institute of Protein Research, Russian Academy of Sciences
Master of Science in Biotechnology and Polymer Chemistry 1989–1995
Moscow State Academy of Fine Chemical Technology Moscow, Russia
Thesis: “Synthesis and Studies of Sequential Compaction of a Random Copolymer of Hydrophilic and Hydrophobic Amino Acid Residues”
QUALIFICATIONS PROFILE
Energetic, highly motivated, dedicated, and assertive professional, offering broad-based expertise in biochemistry/ biophysics, manufacturing, research and development, complemented with strong educational foundation and solid publication track record. Excel at managing multiple studies simultaneously from inception to completion, as well as stellar ability in leading and performing all phases of research. Exemplify remarkable analysis, documentation, research, and advocacy expertise, coupled with effective writing and presentation skills. Accustomed to working with people of diverse cultures and organizational backgrounds. Possess a keen eye for detail with proven capability to multitask in a challenging environment. Bilingual in English and Russian. USA citizen.
Strong background in analyzing protein structure/function, sequence, PTMs
Analysis of PEGylated proteins
Strong expertise in LC-MS and MS/MS, MALDI-MS
Extensive experience with HPLC, FPLC, UPLC, CE, and other separation techniques
Expertise in mammalian, bacterial, and plant protein purification (under GLP and GMP)
General knowledge (through collaboration) of SPR, ITC, DLS.
PCR-based quantitative assay development
A strong record of accomplishments, based on high-impact publications and a patent
Proven creativity and problem-solving skills
Strong written and oral communication skills
Eager to work in a fast-paced, flexible and collaborative team environment
CAREER HIGHLIGHTS
Participated in a successful FDA audit of Udenyca (Neulasta biosimilar) at Coherus Biosciences that resulted in successful drug approval and commercial launch.
Confirmed amino acid sequence and found mutations in key Cepheid enzymes, currently in production.
Published 21 research journal articles (8 as first author), 27 conference presentations (8 as first author, 1 invited talk), and 1 patent.
Develop quantitative PCR-based assays for DNA-polymerase activity (Cepheid)
Developed and implemented purification of DNA polymerase mutants by SCX for activity screening (Stratos Genomics)
Played a key role in the development and transfer of IP in glycoprotein (MAb) production in transgenic plants to an outside company by generating and assembling a package of analytical data, thus achieving $4M revenue growth (Dow Chemical)
Conceptualized and implemented methods, under GLP guidelines, for isolation (RP-HPLC, IEX, SEC), characterization and quantitative analysis (LC-MS, LC-UV) of soybean protein allergens, insecticidal protein toxins, and recombinant enzymes, for Dow AgroSciences Regulatory. Trained in GMP.
Developed a methodology, under GLP, for selective and precise quantitation of <1ppm amounts of esterase enzymes in novel patented paint formulations using HPLC-Fluorescence (Dow Chemical).
Used design-of-experiment (DOE) methodology to optimize purification of a human IgG, under GLP, by Protein-A affinity chromatography with on-line quantitative detection (Dow Chemical).
Used design-of-experiment (DOE) methodology to optimize enzymatic digestion conditions in various experimental settings (Dow Chemical).
Established as an expert in structural characterization of monoclonal antibodies and MAb conjugates, glycoproteins, industrial enzymes, recombinant protein toxins and vaccines, peptides, poly- and oligosaccharides (Dow Chemical).
Identified and characterized a biopolymer in waste-water bulking that caused operational problems in a Dow plant, allowing Dow Chemical to solve a problem that caused losing ~$500M/day due to reduced production.
Mastered hands-on advanced expertise in mass-spectrometry and separation techniques, as applied to protein research.
Built a novel UV-Resonance Raman spectrometer with a novel sample cell for measurements of vibrational characteristics of biomolecules (steady-state & kinetics with nanosecond time resolution) and studied thermodynamics and kinetics of early stages of alpha-helix formation in peptides utilizing UVRR (University of Pittsburgh).
Designed and implemented a novel SEC method for separation of protected peptides in organic solvent (Institute of Protein Research).
Performed comprehensive studies on folding intermediates & stability of Staphylococcal nuclease (Institute of Protein Research).
Reviewed articles for scientific journals such as Biochemistry, Biochimica et Biophysica Acta, J. Chromatography B, Medical Science Monitor, Biotechnology Advances, International Journal for Biotechnology and Molecular Biology Research.
TECHNICAL COMPETENCIES
ESI MS & MS/MS, LC/MS, MALDI-TOF MS & PSD, MALDI TOF-TOF and TDS
Spectroscopy: UV/Vis/IR, FTIR, fluorescence, circular dichroism (CD), UV resonance Raman (steady-state and time-resolved), optical rotation
1D- & 2D-PAGE, HPLC, UPLC, CE-UV & CE-LIF, proteomics applications
Peptide mapping & de-novo sequencing
Oligosaccharide & glycoprotein characterization
Characterization of post-translational modifications (PTMs) in proteins, glycosylation
Protein quantitation
PCR-based quantitative assays development to determine activity of DNA-polymerases in QC setting
Broad array of biochemical and bioanalytical techniques Peptide synthesis (solid phase, solution; including BOC- & Fmoc- strategies, synthesis via NCAs)/chemistry
Peptide & protein isolation/purification/analysis (HPLC, FPLC, UPLC, affinity, ion-exchange, TLC, PAGE, other)
Microwave-assisted proteolysis
Sequencing, molecular cloning, PCR, DNA purification & sequencing DOE using JMP
Mass-spectrometers: Thermo Orbitrap Fusion LUMOS, Thermo OrbiTrap LTQ XL, Thermo Q-Exactive Plus, Waters Xevo G2-S Q-Tof, Bruker Ultraflex MALDI TOF-TOF, Bruker Autoflex MALDI TOF-TOF, ABI Voyager DE-STR MALDI-Tof, Micromass TofSpec 2E MALDI-Tof, Bruker Microflex LT MALDI-Tof, Micromass Q-Tof 2, Waters/Micromass Q-Tof Micro ESI, Agilent 6538 Q-Tof
MS software: MassLynx (Waters), Xcalibur (Thermo), BioPharma Finder (Thermo), PEAKS, ProteinMetrics, MassHunter (Agilent), ProteinScape and Compass (Bruker), Voyager (ABI), GPMAW and other similar.
Lasers: Coherent Infinity Nd:YAGs, Coherent Ar+ & Kr+ ion lasers
Scientific work includes: Characterization of PEGylated proteins, Glycosylation (N- & O-linked), antibodies, plantibodies, post-translational modifications in proteins, protein folding (equilibrium & kinetics), protein thermodynamics/ stability, peptide synthesis, liquid chromatography, proteomics, protein isolation/ purification, protein quantitation by LC-UV, LC-Fluorescence, LC/MS.
Reviewed for scientific journals: Biochemistry, Biochimica et Biophysica Acta, J. Chromatography B, Medical Science Monitor, Biotechnology Advances, International Journal for Biotechnology and Molecular Biology Research
PROFESSIONAL EXPERIENCE
Scientist II 2019 - present
Nektar Therapeutics San Francisco, CA
Detailed analysis of biopharmaceutical protein structure and protein-PEG conjugates by mass-spectrometry (LC/MS): Analysis of intact protein and protein-PEG conjugates, analysis of protein primary structure and PTMs, analysis of PEG site occupancy. LC/MS method development. Similarity studies. Forced degradation studies. Formulation studies. Analysis of biopharmaceutical degradation products. Documenting research data and writing technical reports.
Scientist II 2017 - 2019
Coherus BioSciences Camarillo, CA
Detailed analysis of biopharmaceutical protein structure by mass-spectrometry (LC/MS): Intact protein analysis and analysis of protein primary structure and PTMs. LC/MS method development. Similarity studies. Analysis of biopharmaceutical degradation products. Documenting research data and writing technical reports.
Research Scientist 2015 - 2016
Cepheid Bothell, WA
Detailed analysis of protein structure by mass-spectrometry; protein and peptide sequencing (bottom-up and top-down). Front-end sample preparation, method development. Analysis of PNA, oligonucleotides, and other bio-molecules. PCR-based quantitative assays to determine activity of DNA-polymerases (development of QC release tests).
Research Scientist 2014 - 2015
Stratos Genomics Inc Seattle, WA
Protein engineering, protein purification and analysis, molecular evolution, DNA sequencing, coordinating/ running/ troubleshooting HPLC facility, HPLC method development for oligonucleotides and XNTPs
Bio-Analytical Research Scientist 2000–2013
The Dow Chemical Company Midland, MI
Played a vital role in the development and transfer of IP in glycoprotein production in transgenic plants to an outside company by generating and assembling a package of analytical data, thus achieving $4M revenue growth
Instrumental in establishing capabilities for detailed protein characterization by mass-spectrometry (sequence details, PTMs & other modifications)
Orchestrated company activities/projects in analysis of protein glycosylation
Developed a wide range of methods and performed research and analyses in the areas of agricultural biotechnology, vaccines, antibodies and biopharmaceuticals, crop genetic traits, insecticidal toxins, industrial enzymes, waste water microorganisms, and coating materials
Tasked to develop methodologies for accurate analysis of protein post-translational modifications
Led activities/projects in protein structure analysis by circular dichroism (CD) spectroscopy
Displayed unsurpassed expertise in the technology development for Dow Plant-Based Biopharmaceuticals business
Conceptualized and implemented methods for isolation, characterization and quantitative analysis of soybean protein allergens for Dow AgroSciences regulatory
Collaboratively worked with Dow AgroSciences, Dow Industrial Biotechnology, Dowpharma, Chirotech, Epicyte, Plant Research International (The Netherlands), Centocor, Sunol Molecular/ Altor, Ventria Bioscience, Mapp Biopharmaceutical, University of Pittsburgh, Central Michigan University, University of Arizona, Institute of Protein Research (Russia)
Participated in a massive corporate effort, involving other biopharmaceuticals developers to develop and market biopharmaceuticals, mainly antibodies, produced in transgenic plants in conjunction with multiple teams in other locations both in US and Europe
Subsequently sold the patent to a third party and played a key role in assembling the required technical data package, thus generating $4M for The Dow Chemical Company
Successfully coordinated and executed experimental work to identify and characterize a biopolymer in waste-water bulking that caused operational problems in a Dow plant, allowing Dow to solve a problem that caused losing ~$500M/day due to reduced production
Gained comprehensive experience in the agricultural biotechnology product development and regulatory projects by providing detailed characterization data on the genetically engineered enzymes and other commercially-developed proteins throughout 12.5 years of tenure at Dow
Research Assistant 1996–2000
University of Pittsburgh, Department of Chemistry Pittsburgh, PA
Set strategic direction in building a novel UV-Resonance Raman (UVRR) spectrometer/apparatus with a novel sample cell for measurements of vibrational characteristics of biomolecules (steady-state & kinetics with nanosecond time resolution)
Studied thermodynamics and kinetics of early stages of alpha-helix formation in peptides utilizing UVRR
Research Assistant 1993–1996
Institute of Protein Research Pushchino, Russia
Peptide Chemistry & Protein Physics Groups
Intensified the development of synthetic methods & studied folding properties in synthetic polypeptides
Designed and implemented a novel SEC method for separation of protected peptides in organic solvent
Performed comprehensive studies on folding intermediates & stability of Staphylococcal nuclease
Research Assistant 1992–1993
Shemyakin Institute of Bioorganic Chemistry Moscow, Russia
Laboratory of Polymers for Biotechnology & Biology
Meticulously assessed novel stationary phases and developed methods for HPLC of biomolecules
PROFESSIONAL TRAINING
Thermo Q-Exactive and Fusion Lumos operation, 2019
Bruker Autoflex MALDI TOF-TOF, 2020
Bruker MALDI TOF-TOF and TDS, 2015
Microsoft Excel Tips, Techniques and Shortcuts (National Seminars Training): 2016
Mass Spectrometry short course (J.T. Watson): 2002
NP- & RP- HPLC (Chromatography Inst. of America): 2001
cGMP & Quality Issues for Biopharmaceuticals (Center for Prof. Advancement): 2000
TofSpec 2E Operation (MALDI, Micromass): 2000
PROFESSIONAL AFFILIATIONS
The American Chemical Society (ACS)
The American Society for Mass Spectrometry (ASMS)
HONORS & AWARDS
Dow Best External Publication: 2006
Recognition@Dow Awards: 2005 and 2006
Dow AgroSciences Inventor’s Day Award: 2002
International Soros Science Education Program (ISSEP) Honor Award: 1994, 1995, 1996
PUBLICATIONS, PATENTS & PRESENTATIONS
1 patent,
21 journal publications,
27 conference presentations (Addendum follows)
KEY WORDS: protein structure, biochemistry, protein folding, refolding, sequence characterization, PTM, quantitation, purification, extraction, isolation, production, QC / QA, bioanalytical, analytical methods development, assay development, TaqMan, problem-solving, run analyses, biotechnology, biopharmaceutical, proteomics, MAb, antibodies, plantibodies, vaccines, enzymes, protein thermodynamics / stability, chromatography, LC-UV, LC-Fluorescence, RP-HPLC, NP-HPLC, HPLC, FPLC, UPLC, SEC, IEX, affinity, electrophoresis, SDS-PAGE, 2D-PAGE, IEF, TLC, CE, CE-SDS, CE-LIF, GLP, GMP, cGMP, mass-spectrometry, MALDI-TOF MS & PSD, ESI MS, LC-MS, MS/MS, SPR, ITC, DLS, spectroscopy, UV / Vis / IR, FTIR, fluorescence, circular dichroism (CD), UV resonance Raman (UVRR), optical rotation, peptide mapping & de-novo sequencing, oligosaccharides, glycoproteins, glycosylation, peptide synthesis (solution phase, SPPS), microwave-assisted proteolysis, sequencing, PCR, TaqMan, DNA, RNA, Six Sigma, DOE, JMP, regulatory, research and development.
PATENTS
Kristen Briggs, Todd Glancy, Mich B. Hein, Andrew C. Hiatt, Anton S. Karnoup, W. H. Kerr Anderson, Dayakar Pareddy, Joseph Petolino, Beth Rubin-Wilson, Doug Taylor, Jean L. Roberts “Plant production of immunoglobulins with reduced fucosylation”, US Patent 8,106,169, January 31, 2012
PUBLICATIONS
1.K. Kuppannan, S. Julka, A. Karnoup, D. Dielman, B. Schafer, "2DLC-UV/MS Assay for Simultaneous Quantification of Intact Soybean Allergens: Gly m 4 and HPS (Hydrophobic Protein from Soybean)", Journal of Agricultural and Food Chemistry (2014), Journal of Agricultural and Food Chemistry (2014), Article ASAP, DOI: 10.1021/jf500087s
2.S. Griffin, J. Godbey, T. Oman, S. Embrey, A. Karnoup, K. Kuppannan, B. Barnett, G. Lin, N. Harpham, A. Juba, B. Schafer, R. Cicchillo, "Characterization of Aryloxyalkanoate Dioxygenase-12 (AAD-12), a Non-Heme Fe(II)/α-Ketoglutarate-Dependent Dioxygenase Expressed in Transgenic Soybean and Pseudomonas fluorescens", Journal of Agricultural and Food Chemistry (2013) 61 (27), pp 6589–6596.
3.S. Julka, K. Kuppannan, A. Karnoup, D. Dielman, B. Schafer, S. Young, “Quantification of Gly m 4 Protein, A Major Soybean Allergen, By Two-Dimensional Liquid Chromatography with Ultraviolet and Mass Spectrometry Detection”, Anal. Chem. (2012) 84 (22), pp. 10019–10030.
4.S.E. Permyakov, A.S. Karnoup, A.G. Bakunts, E.A. Permyakov, “Sequence microheterogeneity of parvalbumin pI 5.0 of pike: A mass spectrometric study”, Biochimica et Biophysica Acta Proteins and Proteomics (2009) 1794, pp. 129-136.
5.N.A. Cellar, A.S. Karnoup, D.R. Albers, M.L. Langhorst, S.A. Young, “Immunodepletion of High Abundance Proteins Coupled On-Line with Reversed-Phase Liquid Chromatography: A Novel, Two-Dimensional LC Sample Enrichment and Fractionation Technique for Mammalian Proteomics”, J. Chromatography B (2009) 877, pp.79-85.
6.§ A.S. Karnoup, K. Kuppannan, S.A. Young, “A novel HPLC-UV-MS method for quantitative analysis of protein glycosylation”, J. Chromatography B (2007) 859 (2), pp. 178-191.
7.H. Bakker, G.J.A. Rouwendal, A.S. Karnoup, D.E.A. Florack, G.M. Stoopen, J.P.F.G. Helsper, R. van Ree, I. van Die, D. Bosch, “An antibody produced in tobacco expressing a hybrid 1,4-galactosyltransferase is essentially devoid of plant carbohydrate epitopes”, PNAS (2006) 103 (20), pp. 7577-7582.
8.§ A.S. Karnoup, V. Turkelson, W.H. Kerr Anderson, "O-linked Glycosylation in Maize-Expressed Human IgA1", Glycobiology (2005) 15 (10), pp. 965-981.
9.S.A. Asher, A. Ianoul, G. Mix, M.N. Boyden, A.S. Karnoup, M. Diem, R. Schweitzer-Stenner, “Dihedral psi Angle Dependence of the Amide III Vibration: A Uniquely Sensitive UV Resonance Raman Secondary Structural Probe” / J. Am. Chem. Soc. (2001) 123 (47), pp. 117**-*****.
10.I.K. Lednev, A.S. Karnoup, M.C. Sparrow, S.A. Asher, “Transient UV Raman Spectroscopy Finds No Crossing Barrier Between the Peptide -Helix to Fully Random Coil Conformation” / J. Am. Chem. Soc. (2001) 123, p. 2388-2392.
11.I.K. Lednev, A.S. Karnoup, M.C. Sparrow and S.A. Asher, “Nanosecond UV Resonance Raman Examination of Initial Steps in alpha-Helix Secondary Structure Evolution", Spectroscopy of Biological Molecules: New Directions, 11-12, Kluwer Academic Publishers (1999)
12.I.K.Lednev, A.S.Karnoup, M.C.Sparrow, S.A.Asher, “ -Helix Peptide Folding and Unfolding Activation Barriers: A Nanosecond UV Resonance Raman Study” / J.Am.Chem.Soc. (1999) 121, pp.8074-8086.
13.I.K.Lednev, A.S.Karnoup, M.C.Sparrow, S.A.Asher, “Nanosecond UV Resonance Raman Examination of Initial Steps in -helix Secondary Structure Evolution” / J.Am.Chem.Soc. (1999) 121, pp.4076-4077.
14.V.N. Uversky, A.S. Karnoup, R. Khurana, D.J. Segel, S. Doniach, A.L. Fink, "Association of partly folded intermediates of staphylococcal nuclease induces structure and stability" / Protein Science (1999) 8, pp.161-173.
15.§ A.S.Karnoup, “Toyopearl HW Resins Separate Protected Synthetic Peptides” / The Peak (A TosoHaas Newsletter) (1999) 7(3), pp.4-5.
16.V.N. Uversky, A.S. Karnoup, D.J. Segel, S. Seshadri, S. Doniach, A.L. Fink, "Anion-induced Folding of Staphylococcal Nuclease: Characterization of Multiple Equilibrium Partially Folded Intermediates" / J.Mol.Biol. (1998) 278, pp.879-894.
17.§ A.S.Karnoup, V.N.Uversky, “Precursor of Molten Globule in a Statistical Copolymer of Hydrophilic and Hydrophobic Amino Acids” / Mol.Biology (Moscow) (1998) 32, #3, pp.550-556. (Translation into English: Mol.Biology (Moscow) (1998) 32, #4, pp.543-548.)
18.§ A.S.Karnoup, V.N.Uversky, "Sequential Compaction of a Random Copolymer of Hydrophilic and Hydrophobic Amino Acid Residues." / Macromolecules (1997) 30, #24, pp.7427-7434.
19.§ A.S.Karnoup, V.M.Shiryaev, V.N.Medvedkin, Yu.V.Mitin, "Gel-filtration of Protected Peptides in Dimethylformamide" / Bioorgan. Khim. (1997) 23, #1, pp.56-60. (Translation into english: Russian J.Bioorganic Chem. (1997) 23, #1, pp.51-54)
20.§ A.S.Karnoup, V.M.Shiryaev, V.N.Medvedkin, and Yu.V.Mitin, "Size-Exclusion Chromatography of Protected Synthetic Polypeptide Tandems in Organic Solvent" / J. Peptide Res. (1997) 49, #3, pp.232-239.
21.§ A.S.Karnoup, V.N.Uversky, V.N.Medvedkin, "Synthetic Polyamino Acids and Polypeptides. Preparation by the N-Carboxyanhydride method."/ Bioorgan. Khim.(1996) 22, #8, pp.563-574.(Translation into English: Russian J.Bioorganic Chem. (1996) 22, #8, pp.479-490.)
CONFERENCE PRESENTATIONS
1.S. Julka, K. Kuppannan, A. Karnoup, D. Dielman, B. Schafer, “Simultaneous Quantification of Two Soybean Allergens using a Two Dimensional Liquid Chromatography based Assay”, HPLC 2014, New Orleans, LA. Submitted.
2.A.S. Karnoup, “A Novel HPLC-UV-MS Method for Quantitative Analysis of Protein Glycosylation” (invited speaker), PEGS Summit 2009 (Monoclonal Antibodies section), Boston, MA. April 9, 2009.
3.A.S. Karnoup, K. Kuppannan, S.A. Young, “Analysis of Efficiency of Microwave-Assisted Enzymatic Digestion of Proteins”, ASMS 2008, Denver, CO. June 1-6, 2008.
4.J. Rice, S.E. Keeler, M.R. Badger, B.W. Schafer, D. Camper, A.S. Karnoup, S.A. Young, M.J. Henry, S.R. Webb, “Plant Expressed Influenza Hemagglutinin Characterization and Preliminary Efficacy Against Highly Pathogenic Influenza Virus Isolates”, New Cells for New Vaccines II: Focus on Respiratory Virus Diseases. September 17-19, 2007. Wilmington, DE.
5.W. Ni, B. Schafer, P. Xu, A.S. Karnoup, S.A. Young, R.A. Herman, “Characterization of Cotton Cry1F Degradation Products Using 2-D Polyacrylamide Gel Electrophoresis”, 2007 Purdue AG Genomic Conference. September 10-12, 2007. Purdue University, West Lafayette, IN.
6.S.J. Gluck, A.S. Karnoup, D. Dielman, V.L. Langer, J.G. Green, G.A. Von Wald, S.A. Young, J. Zhu, “Elucidation of Exopolysaccharide Isolated from an Industrial Wastewater Treatment Facility”, WEFTEC 2007. October 2007. San Diego, CA.
7.S. Webb, J. Rice, S. Keeler, B. Schafer, M. Badger, A.S. Karnoup, S.A. Young, M.J. Henry, “Biochemical Characterization of Plant-Cell-Produced Membrane-Bound Glycoprotein Vaccine Antigens”, 2007 Plant-Based Vaccines and Antibodies: Plant Expression Systems for Recombinant Pharmacologics (PBVA 2007). June 18-20, 2007. Verona, Italy.
8.S. Webb, J. Rice, S. Keeler, B. Schafer, P. Song, M.J. Henry, A.S. Karnoup, S.A. Young, “Plant Cell Culture Production of Recombinant Subunit Vaccines: Biochemical Characterization and Regulatory Approval Process”, 2007 Society for In vitro Biology Meeting presentation. June 9-13, 2007. Indianapolis, IN.
9.S.C. Hung, D. Dielman, K. Kuppannan, V.L. Langer, A.S. Karnoup, S.A. Young, “Conformational studies of phosphorylated motif in -casein using hydrogen exchange mass spectrometry”, ASMS 2007, Indianapolis, IN. June 3-7, 2007.
10.A.S. Karnoup, K. Kuppannan, D. Dielman, D.G. McCaskill, N.N. Frawley, “Novel UPLC-UV/MS method for quantitative analysis of protein glycoforms”, ASMS 2007, Indianapolis, IN. June 3-7, 2007.
11.S.R. Webb, C. A. Mihaliak, J.Rice, S. Keeler, C. Mizell, R. Roach, R. Cassell, B. Schafer, M. Badger, M. Henry, A.S. Karnoup, S. Young, “Plant-Cell-Produced Vaccines Antigen Characterization, Efficacy and First Regulatory Approval”, 2nd Annual Vaccine Renaissance. June 7-9, 2006, Providence, RI.
12.A.S.Karnoup, V. Turkelson, W.H. Kerr Anderson, "O-linked Glycosylation in Maize-Expressed Human IgA1", Glycobiology 2005, Annual Conference of The Society for Glycobiology, November 9 to November 12, 2005, Boston, MA (abstract #57). Glycobiology (2005) 15 (11), p. 1201.
13.A.S.Karnoup, I.K.Lednev, M.C.Sparrow, S.A.Asher, “UV resonance Raman studies of isotopically substituted peptides and their thermal denaturation” PITTCON 2000, New Orleans, LA. 12-17 March 2000 (paper #1312).
14.I.K.Lednev, A.S.Karnoup, M.C.Sparrow, S.A.Asher, “Nanosecond time-resolved Raman spectroscopy is a powerful tool for characterizing protein folding intermediates” PITTCON 2000, New Orleans, LA. 12-17 March 2000 (paper #193).
15.M.C.Sparrow, I.K.Lednev, A.S.Karnoup, S.A.Asher, “Lysozyme thermal denaturation: A steady-state and time-resolved UV resonance Raman spectroscopic study” PITTCON 1999, Orlando, Florida. 7-12 March 1999 (paper #1348. Program, p.68)
16.I.K.Lednev, A.S.Karnoup, M.C.Sparrow, S.A.Asher, “Time-resolved UV resonance Raman spectroscopic study of thermal denaturation of -helical proteins and model peptides” PITTCON 1999, Orlando, Florida. 7-12 March 1999 (paper #1154. Program, p.62)
17.S.A.Asher, I.K.Lednev, A.S.Karnoup, M.C.Sparrow, “UV Raman Studies of Protein Structure and Dynamics” PITTCON 1999, Orlando, Florida. 7-12 March 1999 (Bomem-Michelson Award. Program, p.53)
18.A.S.Karnoup, I.K.Lednev, S.A.Asher, “Temperature-induced structural changes in proteins and polypeptides studied by UV resonance Raman spectroscopy” PITTCON 1999, Orlando, Florida. 7-12 March 1999 (paper #513. Program, p.33)
19.I.K.Lednev, A.S.Karnoup, M.C.Sparrow, S.A.Asher, “Fast events in protein folding studied by time-resolved UV resonance Raman spectroscopy: Helix-Coil transition” Biophysical Society. Forty-third Annual Meeting, Baltimore, Maryland. 13-17 February 1999 (Program, p.70; Su-Pos 513) Abstract: Biophys.Journal (1999) 76(1), A119.
20.I.K.Lednev, A.S.Karnoup, M.C.Sparrow, S.A.Asher, “Nanosecond Time-Resolved UV Resonance Raman Spectroscopy is a Novel Approach for Kinetic Studies of Protein Folding”. The 25th Annual Conference of the Federation of Analytical Chemistry and Spectroscopy Societies, Austin, Texas. 11-15 October 1998 (Abstracts, p.109)
21.I.K.Lednev, A.S.Karnoup, M.C.Sparrow, C.H.Munro, S.A.Asher, “Nanosecond Time-Resolved UV Resonance Raman Spectroscopy of Protein Folding” ICORS XVI, Cape Town, South Africa, September 1998 (Proceedings, pp.292-293)
22.A.S.Karnoup, I.K.Lednev, M.C.Sparrow, S.A.Asher, “UV Resonance Raman Studies of Temperature Induced Structural Changes in Proteins and Polypeptides” ICORS XVI, Cape Town, South Africa, September 1998 (Proceedings, pp.286-287)
23.M.C.Sparrow, I.K.Lednev, A.S.Karnoup, S.A.Asher, “Lysozyme Thermal Denaturation: A Steady State and Time Resolved UV Resonance Raman Spectroscopic Study” ICORS XVI, Cape Town, South Africa, September 1998 (Proceedings, pp.152-153)
24.S.A.Asher, Z.Chi, J.S.W.Holtz, I.K.Lednev, A.S.Karnoup, M.C.Sparrow, “UV Resonance Raman Studies of Protein Structure and Dynamics” ICORS XVI, Cape Town, South Africa, September 1998 (Proceedings, pp.11-14)
25.I.K.Lednev, A.S.Karnoup, M.Sparrow, C.H.Munro, S.A.Asher, "Nanosecond Time-Resolved UV Resonance Raman Spectroscopy of Protein Folding" PITTCON 1998, New Orleans, 1-5 March 1998. (Abstracts, p.46).
26.V.N.Uversky, S.Seshadri, D.J.Segel, A.S.Karnoup, A.L.Fink, "Diversity of Folding Intermediates in Globular Proteins: Staphylococcal Nuclease and Apo-mioglobin". The 2nd Johns Hopkins Protein Folding Meeting, Coolfont Conference Center, Berkeley Springs, W.Virginia, USA. 15-18 March 1997 (Abstracts and Poster Session Program, p.7).
27.A.S.Karnoup, V.M.Shiryaev, V.N.Medvedkin, and Yu.V.Mitin, "Gel-filtration of Protected Synthetic Polypeptide Tandems in Organic Solvent" Annual Sci. Conference, Institute of Protein Research RAS, Pushchino, Russia. 7-8 June 1996 (Abstracts and Program, p.4).
Contact this candidate