Esat Yildirim M.Sc. Biotechnology

adiyy1@r.postjobfree.com

adiyy1@r.postjobfree.com 9 guinan st apt 1 Waltham 02451 MA, Green Card Holder 1-857-***-****

SKILLS

DNA Isolation, DNA Cloning, Agarose Gel Electrophoresis, PCR, RT-PCR, RNA Isolation, ELISA, Spectrophotometry, Protein Isolation and Purification, SDS-PAGE, HPLC, FPLC, Flow Cytometry, Western Blotting, Mammalian Cell Culture Techniques,

Bacterial Transformation, Plasmid Transfection, 3D Printing, Snap Gene, BLAST, Molecular Cloning, mRNA Delivery, Knowledge of Project Management, LIMS.

SOP Writing, Documentation, Equipment Calibration, Testing Procedures, Microsoft Office, Communication, Quick Learner, Team Player, Organizational Skills, Leader by Example, Management Skills, Budgeting, Problem Solving, Creativity.

WORK

EXPERIENCE

Biologist II 05/18/2020 – 12/22/2020 Abbvie - Worcester

Responsible for Cell culture updates

Work on leads the optimization, validation, implementation, continuous improvement and global technical support for late stage and commercial manufacturing of therapeutic proteins for AbbVie.

Execute experiments in process development and manufacturing support with supervision.

Perform basic data acquisition and analysis in a timely manner. Assists in experimental designs and data interpretation.

Document work appropriately in lab notebooks and technical memos; contributes to writing technical reports

Maintain lab equipment and optimizes lab operations for efficient use of time and resource.

Troubleshoot equipment and experimental problems.

Assist in continuous improvement activities.

Assist in manufacturing support.

Research Assistant 09/2019 to 01/2020 Kernal Biologics – Cambridge/MA

Description: worked on mRNA based drug research and NASA related microgravity project. During the projects, assisted my supervisor. My main responsibility was to work on the NASA project and take care of the cell culture related steps. Besides that, in a drug development project, also did other experiments which are mentioned below. Also worked on mRNA Delivery.

DNA/ RNA Isolation

mRNA Delivery

RNA Purification via FPLC

RNA quality measurement via 2100 Bio analyzer

HPLC, FPLC, Flow Cytometry

Small animal studies

Cell culture experiment for NASA / CASIS microgravity project

GLP Course Training 07-09/2018 Framingham State University – Framingham/MA

Performing ELISA

Gained Basic Level 3D Printing Experience, SOP Writing skills, Documentation, Equipment Calibration and Testing Procedures.

Internship, 01/2017 to 03/2017 Boston University - Boston/MA

Description: worked on diabetes and assisted to the postdoc.

Performed Protein Isolation and Purification, SDS-PAGE, Western Blot, PCR, mice experimentation.

Internship, 01/2016 to 03/2016 Turgut Ozal University– Ankara/Turkey

Description: Studied diabetes and obesity. Worked on “The iris and the related sub-signal pathways, elucidation of their role in obesity”. Assisted to my supervisor. Also used molecular cloning and other molecular techniques for the project.

Gained experience DNA Isolation, PCR, RT-PCR, Agarose Gel Electrophoresis, Bacterial Transformation, Plasmid Transfection, DNA Cloning, Spectrophotometry, Western Blot, Protein Isolation, Protein Purification and SDS-PAGE.

Molecular Cloning

Internship, 06/2015 to 09/2015 Memorial Genetic Diagnosis Center – Istanbul/Turkey

Description: worked on diagnosis of genetic disorders

In order to diagnose the genetic disease, worked on many patient samples. For example, have done with fish to do PGT (genetic diagnosis for embryos).

Performed DNA Isolation, PCR Methods.

Internship, 01/2015 to 03/2015 Cerrahpasa Medical School, Istanbul – Turkey

Description: assisted to my supervisor for Breast Cancer research. Handled routine technics which are mentioned below.

Carried out DNA Isolation, PCR and Mammalian Cell Culture Methods.

EDUCATION

Master of Science in Biotechnology, 01/2018 to 12/2019 Framingham State University Framingham, MA

Pursuing Education in Biotechnology

Drug Development Course: Learned overview of Drug Development Process.

QA&QC Course: Learned principles of QSR (Quality Systems Requirements) as they apply to the procurement of materials and the manufacture, validation and release of products.

Class of Business Operations Management for Biotechnology:

Learned concepts and techniques for planning, designing, controlling and improving business operations.

Fundemantel steps of project management was learned in the class of project management.

In addition, During the course of GLP, I focused on the use of cell culture in

the production of biologically active products. Also I applied sterility, purification, assay of a final product, and documentation for the project

BS in Molecular Biology and Genetics, 2012 to 2016 Istanbul University Istanbul, Turkey

Research Project: The Pathogenesis Potential Role of MicroRNAs in Thoracic Aortic Dissection (TAD); Result: hsa-miRNA143, hsa-miRNA29 and hsa-miRNA30 might have relation with Thoracic Aortic Dissection(TAD) disease, and might use to create new diagnosis method for TAD.

President of Istanbul University Cofactor Student Club

Member of Cofactor Student Organization

VOLUNTEER WORKS AND LEADERSHİP

Student Club President, 01/2016 to 01/2017 Istanbul University Cofactor Student Club – Istanbul/Turkey

Organized all national and international internships

Organized the “International Life Science Congress” (more than 1000 students attended)

Led a team of more than 50 people

Initiated operational improvements using Agile&Lean methodologies to drive efficiency and reduce cost.

Team and Project management.

Club General Director, 01/2016 to 01/2017 Project Cofactor –Istanbul/Turkey

Organized all national and international internships.

Organized the “International Life Science Congress” which more than 1000 students attended.

Led the team of 50 people

Initiated operational improvements using lean methodologies to drive efficiency and reduce cost

Resolved all issues efficiently to ensure enhanced student satisfaction ratings.

Team and Project management.

ACCOMPLISHMENTS

The 3rd best fencing player in my age group in Turkey.

Graduate PMS (Professional Science Master) program in FSU.

2015-2016, Chair of Cofactor Istanbul University Club- (Founding Chair)

April 2016 II. International Cofactor Life Sciences Congress, Istanbul University

(Administrative Supervisor)

March 2016 Project Cofactor Internship Coordinator

January 2015-January 2016 Project Cofactor Congress Coordinator

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