Vijayprakash Manickam

Washington University in St. Louis-Medical Campus, St. Louis, Missouri, USA

+1-314-***-****, adix7n@r.postjobfree.com, adix7n@r.postjobfree.com EDUCATION Ph.D. in Biotechnology, Bharathiar University, Coimbatore, India 2013-2018 M.Sc. in Cancer Cell and Molecular Biology, University of Leicester, UK 2007-2008 B. Tech (Agricultural Biotechnology), TNAU, Coimbatore, India 2003-2007 RESEARCH

TRAINING

Postdoctoral Associate

Washington University in ST. Louis, USA

Postdoctoral Fellow

CSIR- Institute of Genomics and Integrative Biology, New Delhi, Oct’ 2020 to Present

Nov ’18-Mar’2020

Dr. Manikandan S Lab

Projects: Understanding the progression of atherosclerotic plaque and associated molecular mechanisms during obesity

Doctoral Research Student Apr ’13-Sep ’18

Bharathiar University, Coimbatore, India Dr. Ekambaram Perumal Lab Project: Elucidating the mechanisms involved in the neurotoxicity by iron oxide nanoparticles (Fe2O3-NPs) in Mice.

Research Associate Jun ’11-Mar ’13

GV Bioscience Private Limited, Coimbatore, India

Responsibilities

Responsible for research and production related activities

Standardized protocols for viral disease detection using q-PCR, quality control and conducted trainings

Research Assistant (Junior Research Fellow) Dec ’09-Feb ’11 National Institute of Immunology, New Delhi, India Dr. Pushkar Sharma Lab Project: Mechanism inducing neuronal apoptosis in neurodegenerative disease

Upregulation of MAPK PW and over expression of Cyclin D1 were associated with neurodegenerative condition in differentiated PC12 cells. Cyclin D1 and CDK5 are predominantly located in cytoplasm and nucleus respectively, changes in their location were observed during the induction of neurodegeneration and their relocation was achieved by downregulating the MAPK PW. We observed the same in inducible double stable cell line (PC12) for CYCLIN D1 expression. Thus, suggest that the MAPK PW will maintain the nuclear and cytoplasmic levels of CDK5 and Cyclin D1 respectively and thus to prevents the progression of neurodegenerative disease. Master of Science Sep ’07-Sep ’08

University of Leicester, Leicester, UK Dr. Raj Patel’s Lab Project: Understanding the role of Mad2B during DNA damage in mammalian cells

Mad2B localizes to nucleus and increases binding to Cdc20 following DNA damage. Depletion of Mad2B leads to increase in cytokinesis defects, suggesting that Mad2B suppresses mitotic exit during DNA damage in mammalian cells. PUBLICATIONS

Hypercholesterolemia Impairs Clearance of Extracellular DNA and Promotes Inflammation and Atherosclerotic Plaque Progression UmeshKumar Dhawan, Purbasha Bhattacharya, Sriram Narayanan, Vijayprakash Manickam, Ayush Aggarwal, Manikandan Subramanian doi: https://doi.org/10.1101/2020.10.05.308478 (Under revision in “Circulation”)

Vijayprakash Manickam, Purbasha Bhattacharya, Darmanpreet Singh, Mahesh Gupta, Manikandan Subramanian. Pomegranate peel extract suppresses atherosclerotic plaque progression in Apoe-/- mice. (Manuscript under preparation)

Vijayprakash Manickam, Vasanth Dhakshinamoorthy, Ekambaram Perumal (2019). Iron oxide nanoparticles affects behaviour and monoamine levels in mice. Neurochemical Research. 44:1533-1548.

Vijayprakash Manickam, Vasanth Dhakshinamoorthy, Ekambaram Perumal (2018). Iron oxide nanoparticles induce cell cycle-dependent neuronal apoptosis in mice. Journal of Molecular Neuroscience. 64:352-362.

Vijayprakash Manickam, Madhivadhani Periyasamy, Vasanth Dhakshinamoorthy, Lakshmikanthan Panneerselvam, Ekambaram Perumal (2017). Recurrent exposure to ferric oxide nanoparticles alters myocardial oxidative stress, apoptosis and necrotic markers in male mice. Chemico-Biological interaction, 278:54-64.

Dhakshinamoorthy V, Manickam V*, Perumal E (2017). Neurobehavioural toxicity of iron oxide nanoparticles in mice. Neurotoxicity Research, 32:187–203.

Sundarraj K, Manickam V*, Raghunath A, Periyasamy M, Viswanathan MP, Perumal E

(2017) Repeated exposure to iron oxide nanoparticles cause testicular toxicity in mice. Environmental Toxicology, 32(2):594–608.

*Equally contributed to first authorship

SKILL PROFILE

Microscopy: IVM, High throughput screening using delta vision microscope, SEM, TEM, Confocal and fluorescence microscopy

Animal handling: Mice behavioral experiments, Micro CT, MRI, ECG, oral, intravenous and intraperitoneal administrations, perfusion methods, GTT analysis and microtome sectioning of aortic valve, brain regions, adipose tissue, liver and spleen Cell Biology: Generation of double stable inducible cell lines, Animal cell culture and transfection, sh RNA mediated targeted gene knock down, primary cortical neuronal culture, staining methods (H and E, Collagen, Evans blue, Luxal fast blue) Molecular Biology: Plasmid and genomic DNA isolation, PCR, RNA isolation and cDNA conversion, q-RT-PCR, cloning techniques, protein purification methods, western blotting, immune histo chemistry, immune fluorescence assay and TUNEL assay Microbiology: Nonpathogenic bacterial culture, antibiotic sensitivity test, competence cell preparation and transformation.

Immunology: FACS, ELISA, culturing bone marrow derived macrophages, peritoneal macrophage culture, Isolation and culturing of PBMC, isolation of splenocytes, efferocytosis assay

Bioinformatics: Automated quantification of high throughput data using volocity and Fiji, restriction pattern analysis of DNA fragments, primer designing, DNA and Protein BLAST analysis, DNA to protein translation, ORF determination, DNA sequence analysis and phylogenetic tree construction

Radioactive material handling: Working experience with radioactive isotope αP32 AWARDS

Recipient of UGC-meritorious Fellowship in Life Science for perusing doctoral studies (2013- 2018)

Qualified for National Eligibility Test conducted by Agricultural Scientific Research Board in 2014, New Delhi, India

Qualified for State Eligibility Test conducted by Government of Tamilnadu, in 2016, Tamilnadu, India

Recipient of Best poster award presented at National science day exhibition 2004, conducted by Department of Biotechnology, Government of India

Recipient of Best poster award presented at the National seminar on “Emerging trends in Biological Science” August 18th – 19th, 2005. Department of Biotechnology and Microbiology, Hindusthan College of Arts and Science, Coimbatore

Recipient of Best presentation award at the international conference on biotechnology and healthcare conducted held during October 26-27, 2017, at PJTSAU, Hyderabad, India. Conducted by Cenetri publication group

PAPER PRESENTED

“National conference on Trends in Nanobiotechnology” (NCTN-2016), Haryana Agricultural University, Hisar in collaboration with Biosensor Society and Nano & Molecular Society. November 29-30, 2016. Abstract printed in the proceeding page number 22

“National conference on Advanced Functional Materials” (ncafm-2014), Department of Physics, Bharathiar University, Coimbatore. January 30- 31, 2014. Abstract printed in the proceeding page number 26

“French-Indian Inter-Academic Symposium on infectious Diseases”, November 30th – December 3rd, 2010. NII, New Delhi

“National Seminar on Cancer Biology” November 5th, 2013, organized by Department of Biotechnology, Bharathiar University, Coimbatore

“Cyber Crime and Security” January 7th, 2014. Department of Biotechnology, Bharathiar University, Coimbatore

“Research Methodology” December 10th -13th, 2013. Department of Biotechnology, Bharathiar University, Coimbatore

“Human Resource Development for Biotech Commerce” February 18th – 19th, 2005. PSG college of Technology, Coimbatore

TRAINING AND

WORKSHOP

Workshop on “High Content Screening and Image Analysis for Biosciences”, 27-28 Nov 2018. Organized by THSTI in association with GE Healthcare Lifesciences

“One Day workshop and training programme on ICP-MS” December 18th, 2015, Department of Environmental Science, Bharathiar University, Coimbatore

Summer Training programme, June 12th to August 10th, 2006. Mahyco Research Center, Maharashtra Hybrid seed co. Ltd., Jalna, Maharashtra, India AFFILIATIONS Hon'ble Editorial Board of IJBST Journal Group since April’ 19-present REFFERENCES Dr. Manikandan Subramanian, Senior Lecturer in Cardiovascular Inflammation, Queen Mary University of London, UK

Former senior Scientist at CSIR-IGIB, New Delhi, India. Ph: +91-11-298***** (O), Mobile: +91-965*******,

adix7n@r.postjobfree.com, adix7n@r.postjobfree.com, Dr. Perumal Ekambaram, Associate Professor,

Molecular Toxicology Lab,

Department of Biotechnology, Bharathiar University, Coimbatore- 641 046, Tamilnadu, India.

Ph: 042*-******* (O), Mobile: +91-986*******, adix7n@r.postjobfree.com

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