Hassan GHEISARI, MS OF BIOTECHNOLOGY

Phone:(936-****-***

linkedin.com/in/hassan-gheisari adiw5a@r.postjobfree.com Experienced in the fields of biotechnology and nursing. Seeking for opportunity to expand my knowledge and experiences in the biotechnology industry.

WORK EXPERIENCES

September 2020 Research Assistant, Agrilife Research Center Texas A&M University, COLLEGE STATION, Texas. December 2020 Supervisor : Dr. Michael Thomson

Executing CRISPR/CAS9 gene editing technique in plants, designing sgRNA.

Optimizing protocols to improve efficiency of homology-directed repair for gene editing in rice.

Developing a gene editing protocol for the halophyte species Salicornia (Glasswort).

Conducting genotyping, tissue culture, and optimizing growth media for plants.

Expert in using Belchling, CRISPR-P v2.0, and CRISPRdirect data bases. Jun 2020 Internship (40 H/W), Agrilife Research Center Texas A&M University, COLLEGE STATION, Texas August 2020 Supervisor :Dr. Michael Thomson

Optimizing protocols to improve the efficiency of homology-directed repair for gene editing in rice.

Helping develop a gene editing protocol for the halophyte species Salicornia (glasswort). January 2020 Research Assistant, Agrilife Research Center Texas A&M University, COLLEGE STATION, Texas. May 2020 Supervisor : Dr. Michael Thomson

Conducted CRISPR/CAS9 gene editing technique on rice.Conducted plant tissue culture.

Executed gene cloning, restriction digest, bacterial culture, Plasmid purification. Green Fluorescent Protein (GFP)

Designed PCR Primer, conducting PCR,gel electrophoresis analysis,and gel extraction.

Performed bacterial transformation, PDS gene.

Conducted bioassay in a 96-well plate format and quantifying different proteins. August 2019 Research Assistant, Texas A&M Health Science Center, COLLEGE STATION, Texas. January 2020 Supervisor : Dr. Xu Peng

Explored role of CDC42 GTPase in vasculogenesis and organ specification.

Protein purification screening with anion exchange chromatography and plasmid purification.

Executed SDS-PAGE and Western-blot protein analysis on antibodies and analyzing bands using gel imaging techniques.

Conducted anesthesia, Sonography, and surgery on mice to extract organs and performing RNA iso- lation. (100 mice)

August 2019 Graduate Teaching Assistant, Stephen F.Austin State University Biology Dept., NACOGDOCHES, Texas. August 2018 Supervisor : Dr. Alexandra Van Kley

Directed DNA sequencing analysis to determine bacterial community presence on healthy and un- healthy freshwater sponges.

Directed bacterial, and mammalian cell culture and plate screening. Counting mammalian cell using hemocytometer.

Conducted qPCR, RT-PCR and protein purification screening.

Teaching biology and microbiology to 60 junior undergraduate students per semester. HASSAN GHEISARI - CV 1

July 2013 Anesthesia Assistant, TEHRAN-SHIRAZ, Iran. May 2018 Hospitals : Namazi, Modares and personal office.

Conducted general and regional anesthesia and analgesia treatments on patients, monitoring pa- tient’s vital sign in heart,plastic, general surgeryoperatingroomsandemergencycareunits.(40 hours per week for 4 years)

Led anesthesia team in the operating room, and fifteen junior anesthesia students per semester in operating room. (2 years)

Trained to handle every stressful situation in the operating room and critical care units.

COMPUTER SKILLS, AWARDS & CERTIFICATES

Computer Skills PYTHON, SPSS, Microsoft project, Microsoft Office : Word, PowerPoint and Excel. Award Awarded a merit-based departmental scholarship, Texas A&M 2019-2020. Certificates Certificate of Entrepreneurship & Management, Mays Business School Texas A&M University. Bio-safety level 2, Texas A&M University.

EDUCATION

2019 - 2020 Master of Biotechnology, GPA : 3.9/4.0. Texas A&M University, College Station, Texas.

2018 - 2019 Master of Science in Biotechnology, GPA : 4.0/4.0. Stephen F.Austin State University, Nacogdoches, Texas. 2008 - 2012 Bachelor of Nursing (Anesthesia), GPA : 3.7/4.0. Shahid Beheshti Medical University, Tehran, Iran

REFERENCES

Dr. Michael Thomson

Professor and Chair in International Rice Improvement, TEXAS A&M UNIVERSITY, adiw5a@r.postjobfree.com, +1-979-***-**** Dr. Victor Ugaz

Chair, TAMU Professional Program in Biotechnology, TEXAS A&M UNIVERSITY, adiw5a@r.postjobfree.com, +1-979-***-**** Dr. Toby li

Professor of Management, TEXAS A&M UNIVERSITY, adiw5a@r.postjobfree.com, +1-979-***-**** HASSAN GHEISARI - CV 2

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