Junior Microbiologist

LAKSHMI CHELAKKARA KRISHNAN

** ****** **, **********, **, 06040 Phone: +1-860-***-**** Email: *******.*******@*****.*** Education Summary

Master of Science in Microbiology

Mahathma Gandhi University, Kerala, India Aug 2009 – Sep 2011 Bachelor of Science in Microbiology

Bharathiar University, Tamil Nadu, India June 2006 – Apr 2009 Personal Dossier

Date of Birth 11/30/1988

Visa Status H-4 dependent spouse of H-1B nonimmigrant with Employment Authorization

(Valid till Dec 2021)

Experience Snapshot

Medical Document Reviewer Feb 2014 to June 2015

Junior Microbiologist Nov 2011 to March 2013

Detailed Job Duties

1) Medical Document Reviewer Chennai, India

Rapid Care is an enterprise in Healthcare Information Management, Legal support services, Data Analytics and High-end KPO Services across the globe. One of the key services offered by the company is Medical Record Review which produces a medical chronology, customized to meet client requirements. Being a Medical Document Reviewer, job duties included:

Summarize medical case details in a few lines

Review detailed medical report and summarize the data on Hospital admissions, symptoms, and care compliance

List physicians and facilities, indicating physician specialty

Capture procedures and keynotes from the physician

Separate chronologies prior to injury and post-injury

Ensure that every encounter is covered comprehensively and summarized

Handle additional records related to the case appropriately

Link summaries to respective records in order to facilitate easy navigation and retrieval of source pages

Review and audit all claims, prescriptions, and billing paperwork

Summarize all interrogatories and depositions as appropriate to the case 2) Junior Microbiologist Kochi, India

DDRC SRL Group of Laboratories are pioneers in the field of professional diagnostics and medical laboratories services in the state of Kerala, India. Being a Junior Microbiologist, job duties included:

Preparing media and reagents as needed for laboratory testing

Sterilization of media, glassware and other instruments

Perform Quality Control check

Maintain department stock cultures

Conduct microbiological assay in accordance with Standard Operating Procedures

Isolate subcultures of microbial strains from clinical samples

Microbiological analysis of infectious organisms in clinical samples

Anti-microbial Sensitivity testing by Kirby Bauer method

Quality analysis of water samples by MPN method

Perform staining techniques mainly Acid-Fast staining and Gram staining

Perform Serological tests (manual) like Widal, RPR test, TPHA and card tests for HIV, HBsAg and Typhoid

Perform ELISA techniques for Dengue, Leptospira, ANA, dsDNA, HIV, Hepatitis B and Hepatitis C virus

Interpretation and preparation of reports

Decontaminating infectious waste before disposal.

Projects Undertaken:

Title: Isolation of pigment producing microorganisms from soil and extraction of pigment. Abstract: Samples were collected from rhizosphere and non-rhizosphere part of soil. The pigment producing microorganisms were isolated using dilution plate technique. Isolation of pure cultures of pigment producing microorganisms were done on Yeast extract malt extract agar medium. The pigment producing microorganisms were selected and inoculated into Yeast extract malt extract broth medium and it was maintained at 37 C in a shaking incubator in the presence of light. Pigment producing cells were harvested from the fermentation broth by centrifuging and again recentrifuged in distilled water. The cells were transferred to methanol. Extracellular pigment was extracted easily. In case of intracellular pigment, the suspension containing methanol was kept in boiling water bath for a few minutes. The suspension containing methanol was dried in oven to get the dried pigment. The dry extract of pigment was dissolved in methanol to prepare a stock solution for dyeing. The fabric was immersed in the methanol extract overnight and washed in water and dried in shade.

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