Researcher
Resume:
Wajiha Mumtaj Khalid
LinkedIn: https://www.linkedin.com/in/wajiha-khalid/
email: adhsgq@r.postjobfree.com
adhsgq@r.postjobfree.com
Summary
• Excellent team-player, ambitious personality, quick learner, has confident communication and analytical skills developed through university, regional, and invited presentations.
• Over 5 years of experience in problem solving and strategizing in the area of quantitative systems biology and the relevant industry applications
• Forward-thinking and passionate biochemist with a strong background in cancer biology, cancer heterogeneity, systems biology, and immunofluorescence.
Education
2018-2020 Master of Science in biological Sciences, Fisk University, Nashville, TN. Advisor: Dr. Darren Tyson, Ph.D., Prof. of Biochemistry & Cancer Biology. Vanderbilt University, Nashville, TN
2013–2018 Bachelors of Science in Biochemistry with a Minor in Biology The Florida State University, Tallahassee, FL
Research Experience
2018–2020 Researcher, Vanderbilt University, Nashville, TN
• Studied Small Cell Lung Cancer (SCLC) heterogeneity at the single cell level, both computationally and experimentally.
• Used human derived small cell lung cancer cell lines to conduct immunofluorescence assays in order to see subtypes present at the single cell level*
• Conducted time-dependent drug–response assays using fluorescence microscopic image-based quantification of cell counts in multi-well plates
• Monthly presentation to 30+ scientists consisting of data visualization
• Proficient in Cellavista imaging, and BioTek Gen5 software
• Experienced in oral presentation to 50+ scientists in the Cancer biology department at Vanderbilt University and during thesis defense
• Analyzed Mass Cytometry (CyTOF) data in order to identify gene expression at the single cell level
• Experience in R and Python for data analysis: R for producing and evaluating dose–response curves; Anaconda (as a Python Jupyter Notebook) for analysis of single-cell RNA-seq
• Used Github as a platform to push/pull code and data
• Implanted cancer cells as a single-cell suspension into mice using a prime matrigel formulation to ensure survivability and growth
• Identified mixture of phenotypes at the single cell level through single cell-RNA seq and CyTOF data
• Worked independently to learn computational methods: hierarchical and heat-map clustering
* Experiments was pause due to COVID-19 shutdowns
2016–2018 Researcher, Florida State University College of Medicine, Tallahassee, FL
• Project focused on the microtubule regulator, Dim Gamma Tubulin 4 (Dgt4), and understanding its involvement in micro- tubule organization at the mitotic spindle and kinetochores
• Performed Western Blotting to illustrate the type of mutant that was being researched
• Generated Drosophila CRISPR/Cas9 mutant alleles of Dgt4. Prepped slides and used confocal fluorescent microscopy to analyze the histology of cytoskeleton and cell division of neural stem cells. Evaluation of mutant phenotypes discovered that Dgt4 mutant Drosophila neuroblasts have lost centrosome asymmetry
• Screened mutants looking at expression driven only in drosophila eyes to determine the possible developmental consequences of mutation in dgt4, which led to identifying various phenotypes of the eye and head shape
• Conducted PCR, DNA sampling, electrophoresis, and sequencing on mutant samples Work and Mentoring Experience
2017-2018 International Rescue Committee (IRC) and Migrant + Refugee Education Alliance
(MREA), Florida State University
• Mentored in English to Syrian and Ugandan refugee children in Tallahassee, FL. The goal is to successfully help incoming refugees transition into America/western culture, and understand English in order to communicate in society. Mentoring was held in the family’s house and teaching included learning the sound of the alphabet, reading, writing, numbers, addition, subtraction, and multiplication.
2015-2016 Equipment Inventory Management System, Florida State University
• Goal is to generate a universal database for faculties to identify scientific instruments within FSU. The task consisted of collecting scientific equipment data for FSU-wide inventory management system and grouped them into categories using excel Data Sorting. Sorting included assessed Documentation, pictured 5000+ laboratory equipment items, in all departments at FSU.
Techniques and software
Computer Programming: Python, R.
Publications
M. M. Tillery, B. A. Dietrick, C. Zheng, C. N. Blake-Hedges, R. A. Buchwalter, L. Kao, Y. Zheng, W. M. Khalid, K. E. Huetteman, B. J. Whitehead, T. L. Megraw. Genetic Screen for Centrosomin Synthetic Leathality Reveals Novel Proteins Required for Acentrosomal Cell Division Molecular Biology of the Cell, 28, (2017). Invited Talks
November 2019 Characterization of Small Cell Lung Cancer (SCLC) subtypes Cancer Biology Science Hour, Vanderbilt University, TN Poster Presentations with Q + A
September 2019 7th Annual Bridge Research Celebration Day, Nashville, TN
April 2019 Fisk University Research Symposium 2019, Nashville, TN
April 2018 ACC Meeting of the Minds Boston College 2018, Chestnut Hill,MA
April 2018 Florida State University Spring Symposium 2018 Tallahassee, FL
November 2017 Annual Biomedical Research Conference for Minority Students (ABRCMS), Phoenix, AZ
March 2017 Florida State University Spring Symposium 2017, Tallahassee, FL
Februrary 2017 Florida Undergraduate Research Conference (FURC) 2017, Boca Raton, FL
References
Prof. Darren Tyson
Vanderbilt University
Department of Biochemistry
Nashville, TN 37232
B adhsgq@r.postjobfree.com
Prof. Vito Quaranta
Vanderbilt University
Department of Biochemistry
Nashville, TN 37232
B adhsgq@r.postjobfree.com
Prof. Tim Megraw
Florida State University
Departments of Biomedical sciences and
Neuroscience
Tallahassee, FL 32303
B adhsgq@r.postjobfree.com
Prof. Mark Kearley
Florida State University
Departments of Chemistry and Biochem-
istry
Tallahassee, FL 37203
B adhsgq@r.postjobfree.com
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