Payal Guliani

Researcher

E-mail:adhpjb@r.postjobfree.com

Phone: +917*********

Profile:

To work in a challenging but professional atmosphere which shall enable me to keep in touch with emerging technologies, and provide scope for widening the spectrum of my knowledge and thus serve the organization with my expertise and hard work. Skilled and result-oriented research scholar with solid academic preparation holding master degree and experience in lab management and performance seeks position in risk management. Proven ability to assess and manage complex obstacles; viewed as a strong trouble-shooter. Successful in intense and demanding environment, providing decisive team leadership qualities.

Education

Panjab University:

Master in Stem Cell Tissue Engineering and Biomedical Excellence 2018-2020 Related coursework: Cell and Molecular Biology, Genomics and Proteomics, Immunology, Statistics, Tissue Engineering, Skin and Wound healing, Regenerative medicine, Research Methodology, Intellectual Property Rights.

Bachelor in Science 2015-2018

Major subject- Biochemistry

Minor Subject- Chemistry and botany

Relative Experience

CSIR-NEIST, ASSAM June -September 2020

-Summer Research Training Program Online 2020

- Research work (at home): To study the ageing and behavioural changes of drosophila melanogaster when diet with green tea polyphenols.

- Eminent Faculty lectures on various topics such as sustainable development, Nano technology, cell therapies, respiratory disease, drug discovery and how to make earth a better place

- Drug designing lectures and various software knowledge.

- Presented research results at internship seminars and retreats to audiences ranging from 25 to 100+ people

Stem cell laboratory Jan-April 2020

- To study the effect of citrus sinensis polyphenol extract on Kidney Diseases (paused due to COVID-19)

- Conducted extensive data analysis via SPSS

- Review article on “From waste to regeneration: The osteogenic and chondrogenic potential of orange peel flavonoids in the treatment of osteoarthritis” (Completed and submitted in Panjab University, Chandigarh, Journal)

Biotechnology Lab June 2019

- Post Graduate Institute of Medical education and Research, Chandigarh, India

- Supervisor: DR. D.B Banerjee and department faculty

-Responsible for logging tissue samples

- Initial handling and preparation of various techniques and experiment perform on the day

- Handling Tissue culture lab

-Techniques used: Microtomy, GLP, Microscopy, Protein and nucleic acid estimation via software, Blot analysis

- Culturing and passaging of cell lines

- Report Writing

- Creatinine estimation on patient’s urine then western blot analysis on samples

- Provided research support, case writing and data analysis, managed case submission process, edited, redrafted, and commented on working papers.

- Collaborated with colleagues from department to work on report entitled “The curves of dilution process”. Drafted initial outline and substantial portions of text, performed data analysis, provided research support, produced graphics, managed document throughout drafting process.

Private tutor:

Class IX and X- Science and Social 2019-2020

Class X All subjects 2019

Bachelor of Science- Biochemistry Semester I, II 2016-2017 Presentations

1. Journal club: February, 2020

“Mithramycin A inhibits colorectal cancer growth by targeting cancer stem cells.” Description: Mithramycin-A consists of a tricyclic chromophore with two aliphatic side chains, made of trisaccharide and di-saccharide chains. It induces PARP and Cas-9 mediated cell Death, with concentration 0.1- 100umof Mit-A inhibit osteoclastic bone resorption invitro and inhibit cancer growth by cross-linking GC-rich DNA. 2. Seminar club: August, 2020

“Engineering smart hybrid tissues with built-In electronics” Description: There is a need of a technology that can integrate electronics with engineered tissues to collect data from within the tissue and report on it in real time. The integration of bio molecules with electronic elements such as electrodes, sensors, transistors etc., form multi- functional devices that may able to perform detection, monitoring of biological materials. They convert (bio) chemical information into electronic. Use of various approaches in the field of biomedical electronics implants has evolved from stiff implants to micro-Nano then to thin electronic meshes reaching to the size of a single cell and mechanical properties matching the soft tissue. These overcome the negative effect of foreign devices and its rejection from the body. Use of bionics has various advantages such as controllable lifetime, biodegradable, help diminish the scarcity in donor organs.

3. Project Presentation/viva: September, 2020

“From Waste to Regeneration: The osteogenic and chondrogenic potential of orange peel flavonoids in the treatment of osteoarthritis”

Osteoarthritis is the cause of joint pain, deformity and degeneration. It is caused by injury or loss of cartilage structure and function. Conventional therapies directed towards relieving pain. Mesenchymal stem have remarkable ability of self-renewal and differentiation into chondrocytes. Dietary orange peel flavonoids such as naringin, hesperidin exhibit anti- inflammatory, anti-oxidant, chondroprotective properties and enhances the differentiation and chondrogenic potential of MSCs. Thus, use of orange peel flavonoids with cell- based therapies and Nano-carrier provides sustain drug delivery, enhances osteointegration potential and thus may offer new opportunities and advantages in treatment of OA. Skills

Laboratory:

Molecular biology techniques: Protein determination, purification; Cell organelle marker enzymes; Isolation of plasmid, genomic DNA; Spectrophotometer; chromatography; electrophoresis techniques; radio-isotopic techniques; cell fractionation methods; centrifugation; Microscopy; DNA amplification- PCR; restriction digestion; Northern, Southern & Western blotting, dot blot; genomic and cDNA library construction, RFLP, RAPD, AFLP; Electrophoretic mobility shift assay; CHIP assay; RNAi technique; flow- cytometry, immunohistochemistry and immunocytometry. Immunology: Histology of lymphoid organs; Blood group typing; antigen-antibody interaction by Oucterlony method; separation of WBC from blood by density gradient centrifugation; detection of live/dead WBC using Acridine Orange/Ethidium Bromide staining and indirect fluorescent antibody test.

Stem cell biology: Preparation of feeder cell culture; Isolation of bone marrow stem cells; characterization of mesenchymal stem cell line and differentiation to osteogenic lineage.

Tissue culture: Aseptic preparation of cell culture media; Revival of preserved cells and their cryopreservation; Cell passaging; cell counting and viability; microbial quality control Software: SPSS; Python; Proficient working knowledge of Microsoft Office applications (Word, Excel, PowerPoint and Outlook)

Learn and perform other more advanced tasks, depending on ability and time available, such as animal surgical procedures

Excellent oral and written communication and interpersonal skills. Ability to adjust communication method based on customer situation

Ability to problem-solve and deal with a variety of concrete variables in situations where only limited standardization exists

Comfortable interacting with a wide variety of people with different personalities

Excellent manual dexterity

Meticulous attention to detail and record keeping in laboratory procedures Leadership and Achievements

Rank Holder in Bachelors Semester 1 to 6

Participated in International Conference on Imminent Concerns & Applications (ICONIKA ) 2020, Chascon 2019, Chascon 2017 (Presented a Poster on “LIPOSOMES – Classification and its Characteristics” under Dr. Nidhi Mittal)

Activities:

Volunteer NGO “AAVAHAN”

Awarded as best and active volunteer- 2015-current working as same under President Dr. Meera Sharma.

Member of BLOOD DONATION CAMP COMMITTEE, SD COLLEGE

Part of various science fest held in college as an anchor and extracurricular activities. References:

Dr. Seemha Rai, Chairperson

Centre for the Stm cells and Tissue

Engineering,

Panjab University

T: 098********

E: adhpjb@r.postjobfree.com

Dr. Nidhi Mahajan, Assistant

Professor

Centre for the Stem cells and Tissue

Engineering

Panjab University

T: 981-***-****

E: adhpjb@r.postjobfree.com

Dr. Anuj Gupta, Associate Professor

Centre for the Stem cells and Tissue

Engineering

Panjab University

T:836*******

E: adhpjb@r.postjobfree.com

Dr. Meera Sharma, President

NGO "AAVAHAN", GGDSD

College, Sec-32C, Chandigarh.

T: 987-***-****

Contact this candidate