Dear Sir/Madam

I hope you are healthy and doing great. I am writing this letter to express my interest in the postdoctoral position in your lab. I have recently completed my PhD under the supervision of Dr Adesh K Saini, an alumnus of NIH from Dr Alan G Hinnebuschs lab. The title of my PhD dissertation is ‘Identification of residues in human peroxiredoxin I for their role in maintaining the redox potential in cells’. We used yeast as a model system for this study. Briefly, Peroxiredoxin proteins reduces oxidative/nitrosative stress and acts as chaperone. They play a dual role in neurodegenerative diseases cancer etc. i.e. they promote as well as suppress them under different conditions. By introducing mutations and protein analysis we found that they not only act as peroxidase but also play an important role in elevating the expression of other genes. We also identified a novel residue which is crucial for its chaperone activity. These findings will help in understanding their regulation and dual role in diseases. Apart from my PhD work I was also involved in a project where we developed a working model of Alzheimer’s disease development by inducing circadian rhythm disruption using constant chronic light in Wistar rats. Briefly, we found that constant light disrupts the rhythms of redox and transcriptional oscillations in the rat’s brain which leads to a dysfunction in myriad cellular pathways. During my PhD, I have practiced various biophysical, molecular and cellular techniques. The details of which are mentioned in my CV attached with the mail. If given an opportunity to work in your group I think I can make useful contributions with my technical skills in molecular biology, Yeast genetics and by putting forward new ideas. I also believe that a postdoctoral opportunity in your lab will allow me to expand my expertise and become an independent researcher. I hope to hear from you soon.

Thank you for your time and consideration.

Sincerely,

Ashu Mohammad

C/O Prof. Adesh K Saini

Email ID: adhh94@r.postjobfree.com

LinkedIn Profile- https://www.linkedin.com/in/ashu-mohammad-14637376/ Phone no: +91-701*******

Ashu Mohammad, Ph.D.

adhh94@r.postjobfree.com

+91-701*******

Research interest

Screening and targeting cellular factors which are required for redox homeostasis in a cell. Education

Doctor of Philosophy (July 2014- June 2020), molecular biology, thesis title

‘Identification of residues in human peroxiredoxin I for their role in maintaining the redox potential in cells’ in molecular biology at Shoolini University, India, in the laboratory of Prof. Adesh K. Saini.

Lovely professional university, Phagwara, Punjab

Master of Science in Biotechnology, June 2012, CGPA 7/10 Sidharth Govt. Degree College, Nadaun, Himachal Pradesh Bachelor of Science in life sciences, June 2010, 62% Publications

A. Mohammad, R.V. Saini, R. Kumar, D. Sharma, N.K. Saini, A. Gupta, P. Thakur, C.C. Winterbourn, A.K. Saini, A Curious case of Cysteines in human peroxiredoxin I. Redox Biology., Sept. 2020, DOI: https://doi.org/10.1016/j.redox.2020.101738 R. Kumar, A. Mohammad, R.V. Saini, A. Chahal, C.M. Wong, D. Sharma, S. Kaur, V. Kumar, C.C. Winterbourn, A.K. Saini, Deciphering the in vivo redox behavior of human peroxiredoxins I and II by expressing in budding yeast. Free Radical Biology and Medicine., 2019, DOI: https://doi.org/10.1016/j.freeradbiomed.2019.09.034 A. Sharma, A. Mohammad, A. K. Saini, R. Goyal, Neuroprotective effect of fluoxetine in chronic constant light induced cognitive deficits, oxidative damage and biomarkers of Alzheimer’s disease like pathology in Wistar rats. Molecular Neurobiology., (Revision Sent).

Ashu Mohammad, Adesh K. Saini (2017) Yeast as a model system to study human diseases. InMetabolic Engineering of Bioactive Compounds: Strategies and processes (ed., Vipin Chand Kalia and Adesh K. Saini) Springer Nature Singapore Pte Ltd., Singapore. DOI: 10.1007/978-***-**-****-9_10. ISBN 978-***-**-****-9. Ashu Mohammad, Priyanka Thakur, Rakesh Kumar, Sharanpreet Kaur, Reena V. Saini, Adesh K. Saini. Biological markers for the effects of yoga as a complementary and alternative medicine. Journal of Complementary and Integrative Medicine., 2019, DOI: https://doi.org/10.1515/jcim-2018-0094.

Patent

Adesh K. Saini, Christine C. Winterbourn, Vikas Kumar, Reena V. Saini, Rakesh Kumar and Ashu Mohammad. Novel microbes for assessing in vivo antioxidant status of foods and method thereof (Patent Filed under application number: 201*********). Technical Skills

Protein purification, Western blotting, Fluorescence microscopy, Gene Cloning, Site directed mutagenesis, RNA isolation, c-DNA synthesis, PCR, qRT-PCR, Protein electrophoresis (Native and SDS-PAGE), Cell culture techniques, Animal handling and behavioral assay (Rodents) and Basic Molecular Biology techniques. Previous Position

Worked as Assistant Professor at RPC degree college Behman Diwana, Bathinda, Punjab

(June 2012- January 2014).

Awards and Achievements

1. UGC-ICAR NET May’2014 qualified (Prestigious National level test) 2. GATE March’2014 qualified (Prestigious National level test) 3. Best poster award at international conference, 2017 at Shoolini University. 4. Represented shoolini university at the Gordon research conference on oxidative stress and disease, 2019 at Ventura, California, USA.

Referees

1. Dr. Adesh K. Saini, Department of Biotechnology and Central Research Cell, MMEC, Maharishi Markandeshwar University, Mullana, Ambala, Haryana, India, 133207. email- adhh94@r.postjobfree.com

2. Dr. Christine C. Winterbourn, Centre for Free Radical Research, Department of Pathology and Biomedical Science, University of Otago, Christchurch, New Zealand. adhh94@r.postjobfree.com

3. Dr. Deepak Sharma, Senior Scientist, GNRPC, Institute of Microbial Technology, Chandigarh, India. adhh94@r.postjobfree.com

Declaration

The information I have provided is true to the best of my knowledge and belief. Ashu Mohammad

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