María Daniela Santi

P h a r m a c i s t, C h e m i c a l S c i e n c e s P h . D .

* * * * * s - P o s t d o c t o r a l r e s e a r c h e r

Age: 33 years old

Citizenship: Argentinian and Italian

Current Address: 912, Carlos María Morales Apartment 302

Montevideo, Uruguay (PC 11200)

Cel phone number: +598-********

Email: adg08y@r.postjobfree.com

ResearchGate profile: https://www.researchgate.net/profile/Daniela_Santi

ORCID:

https://orcid.org/0000-

000*-****-****

OBJECTIVE

I am a postdoctoral researcher with 10-years of experience working with medicinal plants, and with the extraction, isolation, and purification of their bioactive secondary metabolites. I performed a large number of bioactivity assays, screening natural compounds as enzymatic inhibitors. Besides, I worked with different melanoma cell lines and primary cultures of neurons and astrocytes as Alzheimer's and Parkinson’s disease models. I like teamwork, I believe that multidisciplinary work is essential, to be able to carry out deep and transcendent studies. Furthermore, I believe that each person gives a unique contribution at a human level to a nice and motivating work environment. I am looking for a position in the Benson’ Lab to continue growing as a researcher in the neurodegenerative diseases field.

KEY SKILLS

-Proven researcher experience in bioactive natural products discovery.

-Extensive knowledge and practical experience in the extraction, isolation, and purification of secondary metabolites, especially flavonoids compounds.

-Working knowledge and practical experience with chromatographic techniques (TLC, CC, HPLC), spectroscopic techniques (NMR1H and 13C 1D and 2D, IR, UV-VIS, CD), and spectrometric techniques (HMRS).

-Practical experience in natural products biological screenings on enzymes involved in disorders and pathologies (mushroom tyrosinase, murine and human melanoma tyrosinase, bovine xanthine oxidase, human

acetylcholinesterase, human GSK-3β).

-Experience in working with different cell lines (melanogenic B16F0 and HBL, and grown-factor differentiated PC12).

-Knowledge in working with Bioinformatics, as Molecular Docking on MOE software, virtual in silico screening using PharmMapper, Shafts, TargetHunter.

-Knowledge in Western blot, ELISA, Immunofluorescence and immunochemistry assays, confocal microscopy.

-Proven record of accomplishment working with multiple research groups.

-Capable of writing and reviewing research documentations such as Papers, Reviews, Book Chapters, Technical Specifications, Technical reports, and Standard operative procedures of assays and specific equipment.

-Ten years of experience in teaching courses on grade, and post grade educational levels.

-Experience in human resources formations, where I developed

management skills.

-Experience in academic management, having participated in departmental

committees of the Department of Pharmaceutical Sciences as a representative of University teachers.

-Experience in the screening of bioactive natural compounds as neuroprotectors in different models of Parkinson's and Alzheimer's diseases.

-Work experience in obtaining and maintaining primary cultures of neurons and astrocytes (from cerebellum, hippocampus, and cortex of

C57B6J mouse, 3xTG-AD mouse, and Wistar and Sprague Dawley rats, from embryos, neonates to adults).

-Excellent engagement and multidisciplinary work skills.

-Soft skills as emotional intelligence, conscious communication, and

motivational spirit in research groups.

WORK EXPERIENCE

Ph.D. researcher and Assistant professor

Pharmacognosy laboratory, Pharmaceutical Sciences Department, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba, Argentina, and Instituto Multidisciplinario de Biología Vegetal (IMBIV-CONICET). Haya de La Torre y Medina Allende. (X5016HUA). Córdoba, Argentina. Phone: +54 0351- 5353865, ext 53357.

~ 2010 – 2019

As a Ph.D. student, I performed a Doctoral research work focused on the search of flavonoids from Latin American Dalea species, as inhibitors of melanogenesis. I also supported the recruitment of new bio scientists. In addition, I was a professor in the Pharmacognosy subject of degree students.

Postdoctoral researcher (CONICET)

Instituto Multidisciplinario de Biología Vegetal (IMBIV-CONICET), and Neurochemistry department, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE). Av. Italia 3318. (12100). Montevideo, Uruguay. 2017. Phone: + 59 2487 1616.

~ 2017 – 2019

During my first postdoctoral fellowship, my responsibilities within this role included the screening of isolated flavonoids on oxidative-induced death models of Parkinson’s and Alzheimer’s diseases, in a primary cerebellar granule neuron culture (CGN), and in the grown-factor differentiated pheochromocytoma cell line (PC12d). In addition, I have developed some works in Bioinformatics, using the molecular docking tool to explain observed pharmacological activities.

Postdoctoral researcher (ANII)

Biomedical R&D CUDIM (Uruguayan center of Molecular Imaging). Av. Dr. Américo Ricaldoni 2010. Montevideo, Uruguay. Since 2019 to 2021. Phone:

+59 2480 3238.

~ 2019 – February 2021

I am currently working in the R&D Biomedical area, searching natural compounds with neuroprotective activity using a novel paradigm that involves a triple transgenic model of Alzheimer’s disease. In addition, I am participating in the characterization of that paradigm as a valid pre-clinical model for that disease.

QUALIFICATIONS

Ph.D. Chemical Sciences (Outstanding)

Pharmacognosy laboratory, Pharmaceutical sciences department, Chemical Sciences Faculty, National University of Cordoba (Argentina), and IMBIV- CONICET

~ 2012-2017

Search of bioactives compounds from Dalea species as melanogenic inhibitors. Orientations: biological drug assays, pharmacognosy studies, pharmacokinetics, statistical methods.

Degree in Pharmacy (First Class Honours)

Chemical Sciences Faculty, National University of Cordoba (Argentina)

~ 2005 - 2009

PUBLICATIONS

Scientific publications

Flavonoids from Dalea elegans: Chemical reassignment and determination of kinetics parameters related to their anti-tyrosinase activity. Mariana Andrea Peralta*, María Daniela Santi*, Alicia Mariel Agnese, María Gabriela Ortega, and José Luis Cabrera. Phytochemistry letters, 10(2014), pp 260-267. *Contributed equally to this publication. http://dx.doi.org/10.1016/j.phytol.2014.10.012

Chemical and bioactivity of flavanones obtained from roots of Dalea pazensis Rusby. María D. Santi, Mariana A. Peralta, Caterine S. Mendoza, José L. Cabrera, and María G. Ortega. Bioorganic and Medicinal Chemistry letters, 27, (2017), pp 1789-1794. http://dx.doi.org/10.1016/j.bmcl.2017.02.058

Xanthine oxidase inhibitory activity of natural and hemisynthetic flavonoids from Gardenia oudiepe (Rubiaceae) in vitro and molecular docking studies. Santi M.D., Paulino Zunini M., Vera B., Bouzidi C., Dumontet V., Abin-Carriquiry A., Grougnet R., Ortega M.G. European Journal of Medicinal chemistry, 143 (2018), pp. 577-582.

https://doi.org/10.1016/j.ejmech.2017.11.071

In vitro biological evaluation and molecular docking studies of natural and semisynthetic flavones from Gardenia oudiepe (Rubiaceae) as tyrosinase inhibitors. Santi M.D., Bouzidi C., Gorod N.S., Puiatti M., Michel S. Grougnet R., Ortega M.G. Bioorganic chemistry, 82 (2019), pp. 241-245. https://doi.org/10.1016/j.bioorg.2018.10.034

In silico and in vitro Approach for the Understanding of the Xanthine Oxidase Inhibitory Activity of Uruguayan Tannat Grape Pomace and Propolis Poliphenols. Elena Alvareda, Federico Iribarne, Victoria Espinosa, Pablo Miranda, Daniela Santi, Sara Aguilera, S. Bustos, Margot Paulino Zunini. Journal of Biophysical Chemistry, 10 (2019), pp. 1-14. https://doi.org/10.4236/jbpc.2019.101001

Melanogenic inhibitory effects of Triangularin in B16F0 melanoma cells, in vitro and molecular docking studies. María D. Santi, Mariana A. Peralta, Marcelo Puiatti c, José Luis Cabrera, and María G. Ortega. Bioorganic and Medicinal Chemistry, 27 (2019), pp. 3722–3728.

https://doi.org/10.1016/j.bmc.2019.06.041

Identification of pyrazolotriazinones as potential agents for hyperuricemia treatment by using in vitro and in silico studies. M.L. Sciu*, M.D. Santi*, C. Roca Magadán, N.E. Campillo, A. Martinez, M.G. Ortega and E.L. Moyano. *Contributed equally to this publication. SN Applied Sciences, 2 (2020) pp. 1-14. https ://doi.org/10.1007/s4245 2-020-2756-6

A new naphthalene derivative with anti-amyloidogenic activity as potential therapeutic agent for Alzheimer's disease. Suchitil Rivera- Marreroa, Alberto Bencomo-Martíneza, Erika Orta Salazarb, Marquiza Sablón- Carrazana, Laura García-Pupo, Florencia Zoppolo, Florencia Arredondo, Rosina Dapueto, María Daniela Santi, Ingrid Kreimerman, Tania Pardo, Laura Reyes, Lídice Galán, Samila León-Chaviano, Luis A. Espinosa-Rodríguez, Roberto Menéndez-Soto del Valle, Eduardo Savio, Sofía Díaz Cintra, Chryslaine Rodríguez-Tanty. Bioorganic and Medicinal Chemistry, 28 (2020) pp. 1-16. https://doi.org/10.1016/j.bmc.2020.115700

Neuroprotective effects of prenylated flavanones isolated from Dalea species, in vitro and in silico studies. Santi, M. D., Arredondo F., Carvalho D., Echeverry, C., Prunell, G., Peralta M., Cabrera J.L., Ortega M.G., Eduardo Savio, Abin-Carriquiry J.A. European Journal of Medicinal Chemistry, 206

(2020), pp. 1-11. https://doi.org/10.1016/j.ejmech.2020.112718

Book chapter

Dalea genus, chemistry and bioactivity studies. Peralta M.A.*, Santi M.D.*, Cabrera J.L., Ortega M.G. Studies in Natural Products Chemistry, Vol 62, Chapter 8, pp. 308-339.*Contributed equally to this publication. https://doi.org/10.1016/B978-0-444-64185-4.00008-3

Sent publications

Synergistic melanogenic inhibitory activity between flavonoids isolated from Dalea elegans and kojic acid on mushroom and murine B16F0 melanoma cells tyrosinase. Frontiers in Pharmacology-Ethnopharmacology. Manuscript ID: 599338.

In process

Review: Prenyl flavonoids as potential agents to cope with antifungal multidrug-resistance in Candida. A state-of-the-art review and future perspectives. Santi M.D, Lombardo Caramello A.E, Cabrera J.L, Ortega M., Peralta M. Drug Resistance Updates.

TEACHING EXPERIENCE: POSTGRADUATE COURSES

Natural products as modulators of proteins related to human diseases and pathogens. UNC, Córdoba, Argentina. Since 19th to 23th of February, 2018 and February, 2019. Duration: 40 hours.

Usefulness of natural products as potential medicinal and food agents. UdelaR, Montevideo, Uruguay. Since 29th of May to 01st of June, 2018 and May, 2019. Duration: 34 hours.

Bioinformatic applied to natural products. UNC, Córdoba, Argentina. Since 10th to 14th of December, 2018. Duration: 40 hours.

POSTGRADUATE FORMATION COURSES

Synthesis and mechanisms of toxicity of fungal metabolites on animal and plant cells. UNC. Since 4th to 8th of July, 2014. Duration: 20 h. Natural products as modulators of proteins related to human diseases and pathogens. UNC. Since 19th to 21th of November, 2014. Duration: 24 h.

Advanced School on Molecular and Cell Biology to Unravel the Physiology/Pathology of Diverse Biological Paradigms. Instituto de Investigaciones Biológicas Clemente Estable (IIBCE), MEC. Uruguay. Since 9th to 20th of November, 2015. Duration: 120 h.

Theoretical-practical course of processing and analysis of fluorescence images using ImageJ/FIJI. Escuela de Posgrado, Facultad de Odontología, UNC. Since 06th to 9th of August, 2018. Duration: 42 h. Theoretical-practical course of laboratory animals. Instituto Pasteur, Montevideo. Since 25th to 29th of March, 2019. Duration: 42 hours.

LENGUAGES

Spanish. Native.

English, Intermediate level. Reading, writing and speaking.

REFERENCES

Dra. Prof. María Gabriela Ortega, Pharmaceutical Sciences department, School of Chemistry sciences, National University of Cordoba (UNC), Multidisciplinary Institute of vegetal biology (IMBIV-ONICET) Córdoba, Argentina. E-mail: adg08y@r.postjobfree.com. Phone number: +54-035*-****-*** INT 53357.

Dra. Prof. Mariana Peralta, Pharmaceutical Sciences department, School of Chemistry sciences, National University of Cordoba (UNC), Multidisciplinary Institute of vegetal biology (IMBIV-ONICET), Córdoba, Argentina. E-mail: adg08y@r.postjobfree.com. Phone number: +54-035*-****-*** INT 53357.

Dr. José Luis Cabrera, Multidisciplinary Institute of vegetal biology (IMBIV- ONICET), Córdoba, Argentina. E-mail: adg08y@r.postjobfree.com. Phone number: +54-035*-******* INT 53357.

Dr. Juan Andrés Abin-Carriquiry, Neurochemistry department, Biological Research Institute “Clemente Estable” (IIBCE), Montevideo, Uruguay. E-mail: adg08y@r.postjobfree.com. Phone number: +598-****-**** INT 123.

Dra. Florencia Arredondo, Biomedical R&D researcher, Uruguayan Center of Molecular Imaging (CUDIM), Ricaldoni 2010, Montevideo, Uruguay. E-mail: adg08y@r.postjobfree.com. Phone number: +598-****-**** INT 242.

Dr. Eduardo Savio, Head of Radiopharmacy R&D, Comité Ejecutivo, Uruguayan Centre for Molecular Imaging (CUDIM). Ricaldoni 2010, Montevideo - Uruguay. E-mail: adg08y@r.postjobfree.com. Phone number:

+598-****-**** - INT. 172.

Dra. Margot Paulino-Zunini. Bioinformatics Department, Chemistry Faculty, Republic University (UdelaR), Av. Gral. Flores 2124, Montevideo, Uruguay. E- mail: adg08y@r.postjobfree.com. Phone number: +598-****-****.

OTHER LIFE-INTERESTS

Body-Mind science, Philosophy, Yoga, Meditation, Reading, Cooking, Running, Contemporary dance.

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