Assistant Engineering

SHIVANGI MUKESH MODI

Boston, MA ****.***@************.*** 669-***-**** https://www.linkedin.com/in/shivangi-modi-53046a170/ EDUCATION:

Masters in Biotechnology September 2018 — August 2020 Northeastern University, Boston GPA: 3.4/4

Courses: Molecular Biology, Biostatistics, Biotech Enterprise, Neuroscience, Cell Biology, Cell Culture, Protein chemistry Bachelor of Engineering in Biotechnology July 2014 — June 2018 Thadomal Shahani Engineering College, Mumbai University CGPI: 9/10 SKILLS:

• Lab Equipment: spectrophotometer, Nano-drop, Centrifuge, Microfuge, Colorimeter, Compound Microscope, Hemocytometer, PCR, Inverted Microscope, Up-right Microscope, Octet QK 384, SEC, FPLC, Ion exchange chromatography, paper chromatography, HPLC (introduction in course work), Vi-Cell

• Lab Techniques: pipetting, Agarose Gel Electrophoresis, SDS-PAGE, PCR, iPCR, PCR Purification Bacterial Culturing, Chromatographic techniques, Immobilization, Immunoassays, Enzyme digestion, enzyme extraction, DNA/RNA extraction, plating, Plant culture techniques, Fluorescence imaging, Bleaching and freezing of c.elegance, Plasmid preps (mini prep, maxi prep, mega prep), transformation, GFP transfection, Affinity measurement using Octet, Quantification of Antibody using Octet, Multicolor Flowcytometry (demonstration), CRISPR/Cpf1 (demonstration), Handling of Mammalian/Human Cell culture and Maintenance

• Bioinformatics Tools: BLAST, FASTA, PDB, Clustal Omega, Pymol, UNIPROT, NCBI databases, SnapGene (beginner)

• Software: MS Word, Excel, PowerPoint, Octet Data Analysis version 11, FlowJo, PRISM

• Language: Java, .Net, Html, C

• Training: Autoclave, Biosafety awareness, Fundamentals of laboratory safety, GLP, Hazardous Waste Management Classroom Training, Laboratory Safety Program Orientation, Radiation Awareness Training, Regulated Medical Waste, Laser Safety

RESEARCH PROJECT: July 2017 — March 2018

Project on Assessment of effects of the Pharmacological Agents in the modulation of Regenerative abilities in Eisenia fetida

• Cultured and acclimatized Eisenia fetida on cellulose and Introduced selected Pharmacological agents into the Earthworms to modulate regeneration

• Performed Paraffin Histology to observe the repair and conclude Regeneration profile

• Extracted the proteins produced in the Earthworms using Chloroform-Methanol Extraction Technique

• Identified the proteins involved in regeneration using Protein Profiling WORK EXPERIENCE:

Momenta Pharmaceuticals/ Antibody discovery Research Co-Op Jan 2020 — Aug 2020

• Digested plasmids with restriction endonucleases and verified clones via electrophoresis

• Column purified digested plasmids prior to cloning experiments

• Performed high throughput subcloning via Gibson Assembly followed by transformation of E. coli and colony minipreps

• Picked colonies and performed plasmid mini prep to confirm the success of subcloning

• Amplified plasmids in NEB Turbo cells grown in Terrific Broth to use for purification of 1.2 mg endo-free plasmid DNA using the Sigma Maxi prep and mega prep kit

• Plasmid transfection and optimization of Expi293 (Human Embryonic kidney/HEK) and ExpiCHO cell lines

• Designed and optimized protocols to determine antibody-antigen kinetic and quantification assays on Octet QK 384

• Measured Affinity of M281 anti-FcRn antibody using HIS1K and Ni-NTA biosensors and analyzed data using data analysis software

• Performed Data analysis and optimization of Octet assays for nM and pM antibody-antigen interactions

• Observed multicolor Flow cytometry on primary cell lines and practiced data analysis on FlowJo

• Shadowed and understood the process of knockdown of DHFR gene from CHO cell lines using CRISPR/cfp1

• Maintained record of data on Electronic Lab Notebook (ELN) Neuro Lab, Northeastern University/ Research Assistant Goals: To figure out if the ectopic outgrowth is Axon or dendrite in C.elegance. July 2019 — December 2019

• Learnt recipe of NGM, seeding and pouring plates

• Performed Bleaching and freezing of C.elegance

• Screened strains under Inverted and up-right microscopes in regards with the respective fluorescence makers

• Figured Phenotype of cross between KHR00a X CG23B ️strains from the Genotype information available & performed cross Godoy’s Lab, Northeastern University/ Volunteer Research Assistant February 2019 — May 2019

• Cultured and handled 75 Acinetobacter baumannii Mutants

• Observed Electron Microscopy of Acinetobacter baumannii Mutants (RecA and Lon Protease Deficient)

• Purified Genomic DNA from individual clones via Chloroform-Phenol Proteinase-k method

• Achieved Self Ligation Using T4 Ligase and Conducted Transposon insertion mapping via iPCR

• Run Western blot to Characterize the Pillai proteins of various mutant strains

• Mentored an Undergraduate with a major in Biology to perform the protocols with the explanation of basic science Dr. Nerurkar's Modern Clinical Laboratory/ Intern June 2017 — July 2017

• Gained hands-on experience on routine biomedical, hematological, immunological and pathology investigations

• Performed technique of Electrophoresis

• Conducted ELISA with HRP conjugated antibody to detect an immobilized antigen

• Implemented the Hemagglutination test using human red blood cells to detect influenza virus PUBLICATIONS:

• Shivangi Modi, Kritika Mukherjee (2019). Project on assessment of effects of the pharmacological agents in the modulation of regenerative abilities in Eisenia Fetida. International Journal of Advance Research, Ideas and Innovations in Technology, 5(3)

EXTRA-CURRICULAR:

• Awarded First position on National level competition for Haute Couture Unmaad '15 - Cultural Festival of IIM

• Awarded Second position on National level competition for Haute Couture Unmaad '16 - Cultural Festival of IIM

• Hall of Fame Thadomal Shahani College of engineering- Apr 2018

• 4th Rank Holder in 2018 Batch of Biotechnology Engineering Thadomal Shahani College of engineering- Feb 2018

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