Microbiology Plant

SHEEBA MADAAN

Email: ******.******@*****.**

Phone: +91-941*******

Address: B-2/1201, Nandini-1 appt., Vesu, Surat-395007, Gujarat

OBJECTIVE:

Innovation and discovery-oriented Research scientist with extensive expertise in Microbiology, Biochemistry and Pathology to acquire advanced knowledge and to explore opportunities in life science. To take up challenging careers and grow consistently along with the organization.

ACADEMIC PROFILE:

COURSE

INSTITUTION

UNIVERSITY

PERCENTAGE YEAR OF PASSING

M.sc

(Microbiology)

Department of Microbiology and Biotechnology Centre

Maharaja Sayajirao University, Vadodara

73.70%

2016

B.sc

(Microbiology)

N.V Patel College Vidhyanagar

Sardar Patel

University

85.80%

2014

Research Experience:

Junior Research Fellow (JRF)

Forest Research Institute (Deemed) to be University

Dehradun

(June 2017 to Feb 2020)

One year Research Thesis on topic entitled “Studying the ISR pathway induced by diazotrophic endophytic bacteria 2,4-DAPG overexpressing Pseudomonas sp. WS5 and wild type Streptomyces spp. in cereals” from 2015-2016 at M.S.U., Baroda under Dr. Archana Gattupali, PGPR specialist.

Scientific Publications in Peer-reviewed Journal

Patel, J. K., Madaan, S., & Archana, G. (2018). Antibiotic producing endophytic Streptomyces spp. colonize above-ground plant parts and promote shoot growth in multiple healthy and pathogen-challenged cereal crops. Microbiological Research. https://doi.org/10.1016/j.micres.2018.06.003

Pandey, S., Rishi, R.R., Jayaraj, R.S.C., Giri, K., Kumar, R., Pandey, A., Juwantha, R., Madaan, S. and Bhandari, M.S., 2019. Fusarium equiseti is associated with the wilt and dieback of Aquilaria malaccensis in Northeast India. Forest Pathology, p.e12489.

Conference Papers / Abstract Publications

1.Joshi, P., Pandey, S., Madaan, S., Juwantha, R., Kapoor, P., 2019. Effect of leaf Blight Disease on the major oil components of Java Citronella and in Vitro screening of Trichoderma isolates from pathogen.

2.Kapoor,P., Juwantha, R., Pandey, S., Joshi, P., Madaan, S., 2019. Studies on leaf spot disease on GmelinaArborea.

3.Kalpana Tyagi, H.S Ginwal, Sheeba Madaan, Amit Pandey (Oct 2018)Dual culture and nursery screening of trichoderma spp. As potential biocontrol agent against alternaria leaf spot of poplar. International Biodiversity Congress-2018, Forest Research Institute, Dehradun.

4.Ratnaboli Bose, Ekta Rai, Sabyasachi Banerjee, Pooja Joshi, Sheeba Madaan, Amit Pandey, Shailesh Pandey 2018. In vitro antagonism of Trichoderma species against Fusarium solani f.sp. dalbergiae.

5.Presented a research paper entitled “Identification of efficient Trichoderma spp. for antagonistic activities against major pathogens of Poplar” at 12th UCOST 2017-2018 on 07th-09th March 2018.

6.Poster presented at Association of Microbiologists of India (A.M.I.), 56th Annual conference 2015, December 07th - 10th, JNU, New Delhi, India.

Academic Project

Project title- Studying the ISR pathway induced by diazotrophic endophytic bacteria 2,4-DAPG overexpressing Pseudomonas sp. WS5 and wild type Streptomyces spp. in cereals.

Abstract- Pseudomonas strains producing the anti-microbial secondary metabolites like DAPG play an important role in the biocontrol of plant diseases. The present study focuses on characterization of secondary metabolites produced by Endophytic diazotrophic Pseudomonas and Streptomyces strains and their in vitro antagonistic ability against rice blast caused by Magnaporthe grisea and sheath blight caused by Rhizoctonia solani in wheat & sorghmm. Induced systemic resistance response in plants were observed by expression of certain genes involved in signalling pathway which gave long term protection ability in plants from infection.

Techniques used- qRT-PCR, HPLC, Live cell imaging, Fluorescence Microscopy.

Achievements

Qualified National level exam ICAR-NET 2017 in Agricultural Microbiology.

Qualified National level exam GATE 2016 in Life Sciences with AIR 467

Minaxi Lalit Science Award test-2013 [ State Rank- 1st]

GSBTM, GSBioN-2013 [Silver Medal]

BAT-2013 [Qualified]

IDEATE-2011 [1st Rank]

Biology Olympiad-2007 [Amongst top 10%]

Presentations and Participations

Attended conference on Climate Change at FRIDU in May 2018.

Participated in Debate on World Environment Day at FRI, Dehradun in June 2017.

Attended Symposium on Climate Change at Vigyan Dham National Science Centre, Dehardun in May 2017.

Attended XIX Common Wealth Forestry International Conference (CFC) from 3rd to 7th April, 2017 at Forest Research Institute, Dehradun, India.

Presented Model on “Fungal diversity” during open house in M.S.U- January 2016.

Participated in seminar on “Environmental Protection” held at N.V.P.A.S-2011.

Participated in Debate- 2012, 2013.

Participated in Quiz by Udaan-2013.

Presented poster on Global warming-2012 [1st rank]

Special Skills and Laboratory Techniques

Microbiology skills: aseptic and sterile techniques, optical microscopy, bacterial staining, plating methods (streak, spread, pour, replica), enumeration and identification of bacteria, media and buffer preparation, water sample analysis by membrane filtration and MPN, food microbiology, diagnostic biochemical tests for enteric bacteria, use of selective media, antibiotic assay (MIC), plaque assay, conjugation, transduction,capillary assay, biofilm assay.

Biochemistry and Cell & Molecular Biology skills: DNA extraction, RNA isolation from different plant parts, plasmid DNA preparation,, agarose gel electrophoresis, SDS-PAGE, q-RT PCR, HPLC, TLC.

Chemistry skills: solution preparation, titrations, extractions filtrations.

Computer skills: Programming (BASIC, extensive use of Word, Excel, Access and PowerPoint), LINUX Ubuntu operating system handling, Bioinformatic tools like- Blast, MEGA 7, EMBOSS, DNASP, Muscle,etc. software learning experience and statistics for data interpretation.

PROFESSIONAL SKILLS:

Good team working skills and ability to network within large organization

Able to contribute positively to the social environment in the department.

Ability to prioritize, multi task, and able to work under strict deadlines environment.

Hard working and punctuality

Organizational and time-management skills learned through working during my dissertation.

Workshop and presentations:

Certificate course in “ Process Development of Industrial Biotechnology Programme” (2013)

Attended one day work shop on advanced molecular techniques by HI media (2014)

Hobbies: Traveling, Playing Badminton, Yoga, Reading, Drawing

Personal Details:

Date of Birth: 17th January 1992

Father’s Name: Mr. Jai Pal Madaan

Mother’s Name: Mrs. Kamani Madaan

Permanent Address: -B 2/1201, Nandini-1 appt., Vesu, Surat-395007, Gujarat.

Nationality: Indian

Languages known: Hindi, English, Gujarati

Declaration:

I hereby declare that the information furnished above is true to the best of my knowledge and belief.

Sheeba Madaan

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