Scientist Assistant

Sean M. Kelly, M.S.

** ******* **. *** *****, IL 60523 630-***-**** *******@*****.***

www.linkedin.com/in/sean-m-kelly-ms

Summary of Qualifications/Expertise

Cell Biologist/Microbiologist with a broad background and scientific and technical expertise in the Ubiquitin-Proteasome System (UPS) and Virology specifically.

Experience in project design/development as well as complex problem solving/troubleshooting in industry and academic environments.

Extensive experience searching the literature and databases for relevant information, and critically evaluating and utilizing this information.

Experience communicating scientific results and concepts in formal and informal meetings and journal clubs as well as written manuscripts.

Experience training and guiding researchers and students.

Technical Skills

Cell Biology:

Cell Culture

Transfection/Transduction of mammalian cells (exogenous protein expression)

Immunoprecipitation

Pulse-chase radiolabeling

SDS-PAGE/Western Blot

RNAi gene silencing

Quantitative RT-PCR

Protein transport and localization by immunofluorescence microscopy, biochemical analyses, and subcellular fractionation

Molecular Biology & Biochemistry:

DNA cloning; amplification and mutagenesis by PCR

Recombinant protein expression and purification by ultrafiltration, dialysis, column chromatography (affinity, gel filtration, ion exchange) by AKTA FPLC from both Eukaryotic and Prokaryotic cells

Surface Plasmon Resonance (SPR) analysis

Assays of protein function (enzymatic assays)

In-vitro transcription & translation

Functional protein microarray develpoment

Amino acid analog substitution

Virology:

Propagation of virions and plaque purification

Virus purification by ultracentrifigation

Generation and selection of vaccinia virus recombinants

Quantitative intercellular membrane fusion assays and various virological assays (HA; HAI)

Computer/Database:

Standard Microsoft Office/Prism/Online Database tools

Extensive literature search and analysis experience

Sean M. Kelly, M.S.

12 Olympia Ct. Oak Brook, IL 60523 630-***-**** *******@*****.***

www.linkedin.com/in/sean-m-kelly-ms

Research and Professional Experience

Freelance Technical Consultant 08/2019-present

ThriveMD, Shaumburg, IL

Discuss science behind some therapies being offered or considered.

Senior Scientist 11/2014 through 4/2015

LifeSensors Inc., Malvern, PA

Preliminarily developed a functional protein microarray as a platform to assay small molecule inhibitors of DUB activity.

Generated ubiquitin mutants for amino acid analog substitution and post-translational labeling by click chemistry modification.

Explored in-vitro ubiquitinion of IgG molecules.

Research Specialist II 10/2010 through 7/2014

Randall Lab

Department of Microbiology

University of Chicago, Chicago, IL

Successfully carried out a screen of small molecule inhibitors of Fatty Acid Synthase to identify those with inhibitory activity to Dengue virus (DNV) infection for further SAR studies in collaboration with Infinty Pharmaceuticals.

Introduced and led a project looking at the potential roles of different ERAD-associated proteins in the replication of Hepatitis C virus (HCV); identified two cellular proteins which are likely involved in establishing HCV infection.

Managed an independent project looking at the interaction between a DNV-encoded protein with human Fatty Acid Synthase (FASN); identified a novel K63-linked ubiquitinated form of FASN which correlates with a novel enzymatic activity of the enzyme. This work waits further development for publication.

Successfully purified several different viral and cellular proteins in support of different projects in the lab, resulting in two completed manuscripts as a co-author.

Graduate Research Assistant 9/1999 through 7/2007

Musil Lab

Oregon Health and Science University, Department of Biochemistry and Molecular Biology, Portland, Oregon

Demonstrated that hyperthermic and oxidative stress affect proteasome-mediated degradation of both cytosolic and ER-localized proteins at the ubiquitination step.

Education

•Masters of Science (M.S.) 2007; Oregon Health and Science University, Portland, OR; Department of Molecular Microbiology and Immunology

• Bachelor of Science (B.S.) 1995; Benedictine University, Lisle, IL; Major: Molecular Biology; Minor: Biochemistry

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